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Article: Localization of p75 neurotrophin receptor in the retina of the adult sd rat: An immunocytochemical study at light and electron microscopic levels

TitleLocalization of p75 neurotrophin receptor in the retina of the adult sd rat: An immunocytochemical study at light and electron microscopic levels
Authors
Issue Date1998
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37090
Citation
Glia, 1998, v. 24 n. 2, p. 187-197 How to Cite?
AbstractThe low-affinity neurotrophin receptor p75(NTR) or p75, is a 75-kDa cell surface glycoprotein that binds all neurotrophins with similar affinity and is thought to help to ensure the specificity of each neurotrophin. In order to better understand the role of p75 and how it is involved in the neurotrophic effects in the retina, we have examined its cellular localization in the adult rat retina by immunocytochemistry at both light and electron microscopic levels. The similarity between the staining pattern of p75 and that of the distribution of Muller cell processes, as marked by antibodies against S-100 and vimentin, suggests that p75 may be on the Muller cell processes and not on the retinal ganglion cells (RGCs) as previously reported. The failure to detect p75 immunoreactivity on Fluoro-Gold retrogradely labeled RGCs in the radially sectioned retinae also indicates that it is not expressed on RGCs. The results from the light microscopic immunohistochemical studies are supported at the ultrastructural level, showing that p75-immunopositive staining is localized on Muller cell processes and not on RGC bodies. Muller cell processes not only form the inner limiting membrane but also partially wrap around the RGC bodies. Our results lead us to conclude that the previously reported immunopositive staining of p75 on RGCs might belong to the surrounding Muller cell processes. Thus, the pathway of neurotrophic effects on RGCs might be, at least partially, through a glial-neuronal pathway rather than on RGCs directly.
Persistent Identifierhttp://hdl.handle.net/10722/67380
ISSN
2015 Impact Factor: 5.997
2015 SCImago Journal Rankings: 3.296
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHu, Ben_HK
dc.contributor.authorYip, HKen_HK
dc.contributor.authorSo, KFen_HK
dc.date.accessioned2010-09-06T05:54:37Z-
dc.date.available2010-09-06T05:54:37Z-
dc.date.issued1998en_HK
dc.identifier.citationGlia, 1998, v. 24 n. 2, p. 187-197en_HK
dc.identifier.issn0894-1491en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67380-
dc.description.abstractThe low-affinity neurotrophin receptor p75(NTR) or p75, is a 75-kDa cell surface glycoprotein that binds all neurotrophins with similar affinity and is thought to help to ensure the specificity of each neurotrophin. In order to better understand the role of p75 and how it is involved in the neurotrophic effects in the retina, we have examined its cellular localization in the adult rat retina by immunocytochemistry at both light and electron microscopic levels. The similarity between the staining pattern of p75 and that of the distribution of Muller cell processes, as marked by antibodies against S-100 and vimentin, suggests that p75 may be on the Muller cell processes and not on the retinal ganglion cells (RGCs) as previously reported. The failure to detect p75 immunoreactivity on Fluoro-Gold retrogradely labeled RGCs in the radially sectioned retinae also indicates that it is not expressed on RGCs. The results from the light microscopic immunohistochemical studies are supported at the ultrastructural level, showing that p75-immunopositive staining is localized on Muller cell processes and not on RGC bodies. Muller cell processes not only form the inner limiting membrane but also partially wrap around the RGC bodies. Our results lead us to conclude that the previously reported immunopositive staining of p75 on RGCs might belong to the surrounding Muller cell processes. Thus, the pathway of neurotrophic effects on RGCs might be, at least partially, through a glial-neuronal pathway rather than on RGCs directly.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37090en_HK
dc.relation.ispartofGLIAen_HK
dc.rightsGlia. Copyright © John Wiley & Sons, Inc.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMicroscopy, Immunoelectronen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReceptor, Nerve Growth Factoren_HK
dc.subject.meshReceptors, Nerve Growth Factor - metabolismen_HK
dc.subject.meshRetina - anatomy & histology - metabolism - ultrasonographyen_HK
dc.subject.meshTissue Embeddingen_HK
dc.titleLocalization of p75 neurotrophin receptor in the retina of the adult sd rat: An immunocytochemical study at light and electron microscopic levelsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0894-1491&volume=24&spage=187&epage=197&date=1998&atitle=Localization+of+p75+neurotrophin+receptor+in+the+retina+of+the+adult+SD+rat:+An+immunocytochemical+study+at+light+and+electron+microscopic+levelsen_HK
dc.identifier.emailYip, HK:hkfyip@hku.hken_HK
dc.identifier.emailSo, KF:hrmaskf@hkucc.hku.hken_HK
dc.identifier.authorityYip, HK=rp00285en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1098-1136(199810)24:2<187::AID-GLIA4>3.0.CO;2-1en_HK
dc.identifier.pmid9728765-
dc.identifier.scopuseid_2-s2.0-0032189125en_HK
dc.identifier.hkuros36987en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032189125&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue2en_HK
dc.identifier.spage187en_HK
dc.identifier.epage197en_HK
dc.identifier.isiWOS:000075538100004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHu, B=35733928400en_HK
dc.identifier.scopusauthoridYip, HK=7101980864en_HK
dc.identifier.scopusauthoridSo, KF=34668391300en_HK

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