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Article: Highly restricted expression of Cre recombinase in cerebellar Purkinje cells

TitleHighly restricted expression of Cre recombinase in cerebellar Purkinje cells
Authors
KeywordsBAC
Cerebellum
Cre recombinase
L7
Pcp2
Purkinje cells
Transgenic mouse
Issue Date2004
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/68503812
Citation
Genesis, 2004, v. 40 n. 1, p. 45-51 How to Cite?
AbstractThe Purkinje neuron, one of the most fascinating components of the cerebellar cortex, is involved in motor learning, motor coordination, and cognitive function. Purkinje cell protein 2 (Pcp2/L7) expression is highly restricted to Purkinje and retinal bipolar cells, where it has been exploited to enable highly specific, Cre recombinase-mediated, site-specific recombination. Previous studies showed that mice carrying a Cre transgene produced by insertion of Cre cDNA into a small 2.88-kb Pcp2 DNA fragment expressed Cre in Purkinje cells; however, some Cre activity was also observed outside the target tissues. Here, we used Red-mediated recombineering to insert Cre cDNA into a 173-kb BAC carrying the entire intact Pcp2 gene, and characterize the resultant BAC/Cre transgenic mice for Cre expression. We show that BAC/Cre transgenic mice have exclusive Cre expression in Purkinje and bipolar cells and nowhere else. These mice will facilitate Purkinje cell and retinal bipolar cell-specific genetic manipulation. © 2004 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/67378
ISSN
2015 Impact Factor: 2.165
2015 SCImago Journal Rankings: 1.324
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, XMen_HK
dc.contributor.authorNg, AHLen_HK
dc.contributor.authorTanner, JAen_HK
dc.contributor.authorWu, WTen_HK
dc.contributor.authorCopeland, NGen_HK
dc.contributor.authorJenkins, NAen_HK
dc.contributor.authorHuang, JDen_HK
dc.date.accessioned2010-09-06T05:54:36Z-
dc.date.available2010-09-06T05:54:36Z-
dc.date.issued2004en_HK
dc.identifier.citationGenesis, 2004, v. 40 n. 1, p. 45-51en_HK
dc.identifier.issn1526-954Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/67378-
dc.description.abstractThe Purkinje neuron, one of the most fascinating components of the cerebellar cortex, is involved in motor learning, motor coordination, and cognitive function. Purkinje cell protein 2 (Pcp2/L7) expression is highly restricted to Purkinje and retinal bipolar cells, where it has been exploited to enable highly specific, Cre recombinase-mediated, site-specific recombination. Previous studies showed that mice carrying a Cre transgene produced by insertion of Cre cDNA into a small 2.88-kb Pcp2 DNA fragment expressed Cre in Purkinje cells; however, some Cre activity was also observed outside the target tissues. Here, we used Red-mediated recombineering to insert Cre cDNA into a 173-kb BAC carrying the entire intact Pcp2 gene, and characterize the resultant BAC/Cre transgenic mice for Cre expression. We show that BAC/Cre transgenic mice have exclusive Cre expression in Purkinje and bipolar cells and nowhere else. These mice will facilitate Purkinje cell and retinal bipolar cell-specific genetic manipulation. © 2004 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/68503812en_HK
dc.relation.ispartofGenesisen_HK
dc.subjectBACen_HK
dc.subjectCerebellumen_HK
dc.subjectCre recombinaseen_HK
dc.subjectL7en_HK
dc.subjectPcp2en_HK
dc.subjectPurkinje cellsen_HK
dc.subjectTransgenic mouseen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCerebellum - enzymologyen_HK
dc.subject.meshChromosomes, Artificial, Bacterialen_HK
dc.subject.meshDNA Primersen_HK
dc.subject.meshDNA, Complementary - geneticsen_HK
dc.subject.meshGene Expression Regulation, Enzymologicen_HK
dc.subject.meshIntegrases - geneticsen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Transgenicen_HK
dc.subject.meshMotor Activityen_HK
dc.subject.meshPurkinje Cells - enzymologyen_HK
dc.titleHighly restricted expression of Cre recombinase in cerebellar Purkinje cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailTanner, JA:jatanner@hku.hken_HK
dc.identifier.emailWu, WT:wtwu@hkucc.hku.hken_HK
dc.identifier.emailHuang, JD:jdhuang@hkucc.hku.hken_HK
dc.identifier.authorityTanner, JA=rp00495en_HK
dc.identifier.authorityWu, WT=rp00419en_HK
dc.identifier.authorityHuang, JD=rp00451en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/gene.20062en_HK
dc.identifier.pmid15354293en_HK
dc.identifier.scopuseid_2-s2.0-10644262893en_HK
dc.identifier.hkuros94998en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-10644262893&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue1en_HK
dc.identifier.spage45en_HK
dc.identifier.epage51en_HK
dc.identifier.isiWOS:000224257500007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhang, XM=37092286900en_HK
dc.identifier.scopusauthoridNg, AHL=12445077000en_HK
dc.identifier.scopusauthoridTanner, JA=35513993000en_HK
dc.identifier.scopusauthoridWu, WT=7407081122en_HK
dc.identifier.scopusauthoridCopeland, NG=35374759300en_HK
dc.identifier.scopusauthoridJenkins, NA=35379887700en_HK
dc.identifier.scopusauthoridHuang, JD=8108660600en_HK

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