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Article: Effect of insulin-like growth factor 1 on PHA-stimulated cord blood mononuclear cell telomerase activity

TitleEffect of insulin-like growth factor 1 on PHA-stimulated cord blood mononuclear cell telomerase activity
Authors
KeywordsCord blood cell
IGF-1
Regulation
Replicative potential
Telomerase
Issue Date1999
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJH
Citation
British Journal Of Haematology, 1999, v. 104 n. 4, p. 785-794 How to Cite?
AbstractTelomerase may contribute to the capacity for cell replication by compensating for the loss of telomere length. Exploring the use of biological modifiers in increasing cellular replicative potential through telomerase activity may be useful for in vitro expansion of haemopoietic stem cells for transplantation or lymphoid cells for adoptive immunotherapy. In this study we showed for the first time that insulin-like growth factor 1 (IGF-1) modulated telomerase activity in human cord blood mononuclear cells (MNC) and some of the known functional determinants of telomerase activity. We found that cord blood MNC expressed constitutively a low level of telomerase activity and human telomerase reverse transcriptase (hTRT) mRNA, and a high level of human telomerase RNA component (hTR) and telomerase-associated protein-1 (TP1) mRNA. Interestingly, IGF-I alone did not increase the telomerase activity of cord blood MNC but could enhance the PHA-induced increase in telomerase activity. These alterations in telomerase activity were not completely in phase with those of proliferation response. On the other hand, IGF-I did not alter hTRT mRNA expression but enhanced the PHA- induced increase in hTRT whereas TP1 mRNA expression was stimulated by either IGF-I or PHA but showed no additive increase when stimulated by both IGF-1 and PHA. Neither IGF-1 nor PHA altered hTR expression. Finally, the temporal dynamics of hTRT mRNA expression and telomerase activity in cord blood MNC over 5 d in culture were not totally concordant, suggesting that key factors other than hTRT were involved in regulating telomerase activity in cord blood MNC. The modulatory effect of IGF-1 on telomerase activity supports its potential role in increasing replicative potential of cord blood lymphoid cells or haemopoietic stem cells.
Persistent Identifierhttp://hdl.handle.net/10722/67366
ISSN
2015 Impact Factor: 5.401
2015 SCImago Journal Rankings: 2.313
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTu, Wen_HK
dc.contributor.authorZhang, DKen_HK
dc.contributor.authorCheung, PTen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorLau, YLen_HK
dc.date.accessioned2010-09-06T05:54:29Z-
dc.date.available2010-09-06T05:54:29Z-
dc.date.issued1999en_HK
dc.identifier.citationBritish Journal Of Haematology, 1999, v. 104 n. 4, p. 785-794en_HK
dc.identifier.issn0007-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67366-
dc.description.abstractTelomerase may contribute to the capacity for cell replication by compensating for the loss of telomere length. Exploring the use of biological modifiers in increasing cellular replicative potential through telomerase activity may be useful for in vitro expansion of haemopoietic stem cells for transplantation or lymphoid cells for adoptive immunotherapy. In this study we showed for the first time that insulin-like growth factor 1 (IGF-1) modulated telomerase activity in human cord blood mononuclear cells (MNC) and some of the known functional determinants of telomerase activity. We found that cord blood MNC expressed constitutively a low level of telomerase activity and human telomerase reverse transcriptase (hTRT) mRNA, and a high level of human telomerase RNA component (hTR) and telomerase-associated protein-1 (TP1) mRNA. Interestingly, IGF-I alone did not increase the telomerase activity of cord blood MNC but could enhance the PHA-induced increase in telomerase activity. These alterations in telomerase activity were not completely in phase with those of proliferation response. On the other hand, IGF-I did not alter hTRT mRNA expression but enhanced the PHA- induced increase in hTRT whereas TP1 mRNA expression was stimulated by either IGF-I or PHA but showed no additive increase when stimulated by both IGF-1 and PHA. Neither IGF-1 nor PHA altered hTR expression. Finally, the temporal dynamics of hTRT mRNA expression and telomerase activity in cord blood MNC over 5 d in culture were not totally concordant, suggesting that key factors other than hTRT were involved in regulating telomerase activity in cord blood MNC. The modulatory effect of IGF-1 on telomerase activity supports its potential role in increasing replicative potential of cord blood lymphoid cells or haemopoietic stem cells.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJHen_HK
dc.relation.ispartofBritish Journal of Haematologyen_HK
dc.rightsBritish Journal of Haematology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subjectCord blood cellen_HK
dc.subjectIGF-1en_HK
dc.subjectRegulationen_HK
dc.subjectReplicative potentialen_HK
dc.subjectTelomeraseen_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshDNA-Binding Proteinsen_HK
dc.subject.meshFetal Blood - cytology - enzymologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInsulin-Like Growth Factor I - pharmacologyen_HK
dc.subject.meshMonocytes - cytology - enzymologyen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshRNAen_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshTelomerase - metabolismen_HK
dc.titleEffect of insulin-like growth factor 1 on PHA-stimulated cord blood mononuclear cell telomerase activityen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0007-1048&volume=104&spage=785&epage=794&date=1999&atitle=Effect+of+insulin-like+growth+factor+1+on+PHA-stimulated+cord+blood+mononuclear+cell+telomerase+activityen_HK
dc.identifier.emailTu, W:wwtu@hkucc.hku.hken_HK
dc.identifier.emailCheung, PT:ptcheung@hkucc.hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_HK
dc.identifier.authorityTu, W=rp00416en_HK
dc.identifier.authorityCheung, PT=rp00351en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10192441-
dc.identifier.scopuseid_2-s2.0-0033003249en_HK
dc.identifier.hkuros40398en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033003249&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume104en_HK
dc.identifier.issue4en_HK
dc.identifier.spage785en_HK
dc.identifier.epage794en_HK
dc.identifier.isiWOS:000079405500021-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTu, W=7006479236en_HK
dc.identifier.scopusauthoridZhang, DK=7405361705en_HK
dc.identifier.scopusauthoridCheung, PT=7202595465en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK

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