Article: Latent membrane protein-1 of Epstein-Barr virus inhibits cell growth and induces sensitivity to cisplatin in nasopharyngeal carcinoma cells

File Download Links for fulltext
(May Require Subscription)
Supplementary

  • Basic View
  • Metadata View
  • XML View
TitleLatent membrane protein-1 of Epstein-Barr virus inhibits cell growth and induces sensitivity to cisplatin in nasopharyngeal carcinoma cells
AuthorsLiu, Y1
Wang, X1
Lo, AKF1
Wong, YC1
Cheung, ALM1
Tsao, SW1
Issue Date2002
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
CitationJournal Of Medical Virology, 2002, v. 66 n. 1, p. 63-69 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jmv.2112
AbstractNasopharyngeal carcinoma is closely associated with Epstein-Barr virus (EBV) and the EBV encoded latent membrane protein-1 expression (LMP1) is commonly found in the tumour cells. LMP1 has been shown to be involved in modulation of cell growth in B cells but the biological properties of LMP1 expression in nasopharyngeal carcinoma cells are less defined. In this study, a full length LMP1 gene was introduced into an EBV negative nasopharyngeal carcinoma cell line, CNE2, and five LMPl-expressing clones were isolated. Expression of LMP1 did not confer cell growth advantage in CNE2 cells; instead, it induced growth inhibition both in vitro and in vivo. In addition, the LMP1 transfected cells were more susceptible to cisplatin-induced cell death and showed 1.4-4.0-fold increased sensitivity to cisplatin compared to the vector infected control clones. The effect of LMP1 on the balance of Bcl-2 and Bax ratio may play a role in inducing susceptibility to cisplatin-induced cell death. These results demonstrated that LMP1 did not confer growth advantage in CNE2 cells, suggesting that expression of LMP1 may not be crucial in sustaining cell growth in established cell lines. Alternatively, LMP1 alone may not be sufficient to facilitate nasopharyngeal carcinoma cell growth and additional oncogenic factors may be needed along with LMP1 in modulating the malignant property of nasopharyngeal carcinoma. © 2002 Wiley-Liss, Inc.
ISSN0146-6615
2011 Impact Factor: 2.82
2011 SCImago Journal Rankings: 0.267
DOIhttp://dx.doi.org/10.1002/jmv.2112
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLiu, Y
dc.contributor.authorWang, X
dc.contributor.authorLo, AKF
dc.contributor.authorWong, YC
dc.contributor.authorCheung, ALM
dc.contributor.authorTsao, SW
dc.date.accessioned2010-09-06T05:54:27Z
dc.date.available2010-09-06T05:54:27Z
dc.date.issued2002
dc.description.abstractNasopharyngeal carcinoma is closely associated with Epstein-Barr virus (EBV) and the EBV encoded latent membrane protein-1 expression (LMP1) is commonly found in the tumour cells. LMP1 has been shown to be involved in modulation of cell growth in B cells but the biological properties of LMP1 expression in nasopharyngeal carcinoma cells are less defined. In this study, a full length LMP1 gene was introduced into an EBV negative nasopharyngeal carcinoma cell line, CNE2, and five LMPl-expressing clones were isolated. Expression of LMP1 did not confer cell growth advantage in CNE2 cells; instead, it induced growth inhibition both in vitro and in vivo. In addition, the LMP1 transfected cells were more susceptible to cisplatin-induced cell death and showed 1.4-4.0-fold increased sensitivity to cisplatin compared to the vector infected control clones. The effect of LMP1 on the balance of Bcl-2 and Bax ratio may play a role in inducing susceptibility to cisplatin-induced cell death. These results demonstrated that LMP1 did not confer growth advantage in CNE2 cells, suggesting that expression of LMP1 may not be crucial in sustaining cell growth in established cell lines. Alternatively, LMP1 alone may not be sufficient to facilitate nasopharyngeal carcinoma cell growth and additional oncogenic factors may be needed along with LMP1 in modulating the malignant property of nasopharyngeal carcinoma. © 2002 Wiley-Liss, Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Medical Virology, 2002, v. 66 n. 1, p. 63-69 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jmv.2112
dc.identifier.doihttp://dx.doi.org/10.1002/jmv.2112
dc.identifier.epage69
dc.identifier.hkuros74658
dc.identifier.isiWOS:000172560100010
dc.identifier.issn0146-6615
2011 Impact Factor: 2.82
2011 SCImago Journal Rankings: 0.267
dc.identifier.issue1
dc.identifier.openurl
dc.identifier.pmid11748660
dc.identifier.scopuseid_2-s2.0-0036186372
dc.identifier.spage63
dc.identifier.urihttp://hdl.handle.net/10722/67362
dc.identifier.volume66
dc.languageeng
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Medical Virology
dc.relation.referencesReferences in Scopus
dc.rightsJournal of Medical Virology. Copyright © John Wiley & Sons, Inc.
dc.subject.meshAntineoplastic Agents - pharmacology
dc.subject.meshCell Division
dc.subject.meshCisplatin - pharmacology
dc.subject.meshHerpesvirus 4, Human - metabolism
dc.subject.meshHumans
dc.subject.meshIntracellular Signaling Peptides and Proteins
dc.subject.meshNasopharyngeal Neoplasms - genetics - metabolism - pathology
dc.subject.meshNuclear Proteins
dc.subject.meshProteins - genetics - metabolism
dc.subject.meshProto-Oncogene Proteins - genetics - metabolism
dc.subject.meshProto-Oncogene Proteins c-bcl-2 - genetics - metabolism
dc.subject.meshTransfection
dc.subject.meshTumor Cells, Cultured
dc.subject.meshViral Matrix Proteins - genetics - metabolism - physiology
dc.subject.meshbcl-2-Associated X Protein
dc.titleLatent membrane protein-1 of Epstein-Barr virus inhibits cell growth and induces sensitivity to cisplatin in nasopharyngeal carcinoma cells
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong