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Article: Latent membrane protein-1 of Epstein-Barr virus inhibits cell growth and induces sensitivity to cisplatin in nasopharyngeal carcinoma cells
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TitleLatent membrane protein-1 of Epstein-Barr virus inhibits cell growth and induces sensitivity to cisplatin in nasopharyngeal carcinoma cells
 
AuthorsLiu, Y1
Wang, X1
Lo, AKF1
Wong, YC1
Cheung, ALM1
Tsao, SW1
 
Issue Date2002
 
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
 
CitationJournal Of Medical Virology, 2002, v. 66 n. 1, p. 63-69 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jmv.2112
 
AbstractNasopharyngeal carcinoma is closely associated with Epstein-Barr virus (EBV) and the EBV encoded latent membrane protein-1 expression (LMP1) is commonly found in the tumour cells. LMP1 has been shown to be involved in modulation of cell growth in B cells but the biological properties of LMP1 expression in nasopharyngeal carcinoma cells are less defined. In this study, a full length LMP1 gene was introduced into an EBV negative nasopharyngeal carcinoma cell line, CNE2, and five LMPl-expressing clones were isolated. Expression of LMP1 did not confer cell growth advantage in CNE2 cells; instead, it induced growth inhibition both in vitro and in vivo. In addition, the LMP1 transfected cells were more susceptible to cisplatin-induced cell death and showed 1.4-4.0-fold increased sensitivity to cisplatin compared to the vector infected control clones. The effect of LMP1 on the balance of Bcl-2 and Bax ratio may play a role in inducing susceptibility to cisplatin-induced cell death. These results demonstrated that LMP1 did not confer growth advantage in CNE2 cells, suggesting that expression of LMP1 may not be crucial in sustaining cell growth in established cell lines. Alternatively, LMP1 alone may not be sufficient to facilitate nasopharyngeal carcinoma cell growth and additional oncogenic factors may be needed along with LMP1 in modulating the malignant property of nasopharyngeal carcinoma. © 2002 Wiley-Liss, Inc.
 
ISSN0146-6615
2013 Impact Factor: 2.217
 
DOIhttp://dx.doi.org/10.1002/jmv.2112
 
ISI Accession Number IDWOS:000172560100010
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLiu, Y
 
dc.contributor.authorWang, X
 
dc.contributor.authorLo, AKF
 
dc.contributor.authorWong, YC
 
dc.contributor.authorCheung, ALM
 
dc.contributor.authorTsao, SW
 
dc.date.accessioned2010-09-06T05:54:27Z
 
dc.date.available2010-09-06T05:54:27Z
 
dc.date.issued2002
 
dc.description.abstractNasopharyngeal carcinoma is closely associated with Epstein-Barr virus (EBV) and the EBV encoded latent membrane protein-1 expression (LMP1) is commonly found in the tumour cells. LMP1 has been shown to be involved in modulation of cell growth in B cells but the biological properties of LMP1 expression in nasopharyngeal carcinoma cells are less defined. In this study, a full length LMP1 gene was introduced into an EBV negative nasopharyngeal carcinoma cell line, CNE2, and five LMPl-expressing clones were isolated. Expression of LMP1 did not confer cell growth advantage in CNE2 cells; instead, it induced growth inhibition both in vitro and in vivo. In addition, the LMP1 transfected cells were more susceptible to cisplatin-induced cell death and showed 1.4-4.0-fold increased sensitivity to cisplatin compared to the vector infected control clones. The effect of LMP1 on the balance of Bcl-2 and Bax ratio may play a role in inducing susceptibility to cisplatin-induced cell death. These results demonstrated that LMP1 did not confer growth advantage in CNE2 cells, suggesting that expression of LMP1 may not be crucial in sustaining cell growth in established cell lines. Alternatively, LMP1 alone may not be sufficient to facilitate nasopharyngeal carcinoma cell growth and additional oncogenic factors may be needed along with LMP1 in modulating the malignant property of nasopharyngeal carcinoma. © 2002 Wiley-Liss, Inc.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Medical Virology, 2002, v. 66 n. 1, p. 63-69 [How to Cite?]
DOI: http://dx.doi.org/10.1002/jmv.2112
 
dc.identifier.doihttp://dx.doi.org/10.1002/jmv.2112
 
dc.identifier.epage69
 
dc.identifier.hkuros74658
 
dc.identifier.isiWOS:000172560100010
 
dc.identifier.issn0146-6615
2013 Impact Factor: 2.217
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid11748660
 
dc.identifier.scopuseid_2-s2.0-0036186372
 
dc.identifier.spage63
 
dc.identifier.urihttp://hdl.handle.net/10722/67362
 
dc.identifier.volume66
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Medical Virology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Medical Virology. Copyright © John Wiley & Sons, Inc.
 
dc.subject.meshAntineoplastic Agents - pharmacology
 
dc.subject.meshCell Division
 
dc.subject.meshCisplatin - pharmacology
 
dc.subject.meshHerpesvirus 4, Human - metabolism
 
dc.subject.meshHumans
 
dc.subject.meshIntracellular Signaling Peptides and Proteins
 
dc.subject.meshNasopharyngeal Neoplasms - genetics - metabolism - pathology
 
dc.subject.meshNuclear Proteins
 
dc.subject.meshProteins - genetics - metabolism
 
dc.subject.meshProto-Oncogene Proteins - genetics - metabolism
 
dc.subject.meshProto-Oncogene Proteins c-bcl-2 - genetics - metabolism
 
dc.subject.meshTransfection
 
dc.subject.meshTumor Cells, Cultured
 
dc.subject.meshViral Matrix Proteins - genetics - metabolism - physiology
 
dc.subject.meshbcl-2-Associated X Protein
 
dc.titleLatent membrane protein-1 of Epstein-Barr virus inhibits cell growth and induces sensitivity to cisplatin in nasopharyngeal carcinoma cells
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong