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Article: Expression of human oviductin in an immortalized human oviductal cell line

TitleExpression of human oviductin in an immortalized human oviductal cell line
Authors
KeywordsConfocal microscopy
Human oviductin
OE-E6/E7 cells
Oviduct-specific glycoprotein
Oviductal cells
Oviductal secretion
RT-PCR
Issue Date2005
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2005, v. 84 SUPPL. 2, p. 1095-1103 How to Cite?
AbstractObjective: To determine whether OE-E6/E7, an immortalized human oviductal epithelial cell line, expresses oviductin messenger RNA (mRNA) and its translated protein. Design: Transmission electron microscopy was employed to characterize the morphology of OE-E6/E7 cells followed by reverse-transcription polymerase chain reaction (PCR) analysis of oviductin mRNA and sequencing of the nested-PCR product. Confocal microscopy was used, using a polyclonal antibody against human oviductin and Con A as a marker for mannose residues, to reveal the colocalization of human oviduct-specific glycoprotein with the endoplasmic reticulum and Golgi compartments. Setting: University-based anatomy and cell biology department. Patient(s): Women undergoing laparoscopy for tubal ligation or hysterectomy due to uterine fibroma. Intervention(s): An immortalized OE-E6/E7 cell line was previously established using human oviductal epithelial cells. Electron microscopy, RT-PCR, sequencing, immunohistochemistry and confocal microscopy were performed. Main Outcome Measure(s): The presence of human oviductin mRNA and protein in OE-E6/E7 cells. Result(s): OE-E6/E7 cells retain morphological features characteristic of secretory cells and express human oviductin mRNA and its translated protein. Conclusion(s): OE-E6/E7 cells were characterized for the first time by electron microscopy and shown to exhibit histological features typical of secretory cells. Reverse-transcription PCR with sequencing and confocal microscopy showed, respectively, that human oviductin mRNA and protein are expressed in OE-E6/E7 cells. Our results suggest that OE-E6/E7 could be a useful tool for future studies of the function of human oviductin. ©2005 by American Society for Reproductive Medicine.
Persistent Identifierhttp://hdl.handle.net/10722/67348
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLing, Len_HK
dc.contributor.authorLee, YLen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorKan, FWKen_HK
dc.date.accessioned2010-09-06T05:54:19Z-
dc.date.available2010-09-06T05:54:19Z-
dc.date.issued2005en_HK
dc.identifier.citationFertility And Sterility, 2005, v. 84 SUPPL. 2, p. 1095-1103en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67348-
dc.description.abstractObjective: To determine whether OE-E6/E7, an immortalized human oviductal epithelial cell line, expresses oviductin messenger RNA (mRNA) and its translated protein. Design: Transmission electron microscopy was employed to characterize the morphology of OE-E6/E7 cells followed by reverse-transcription polymerase chain reaction (PCR) analysis of oviductin mRNA and sequencing of the nested-PCR product. Confocal microscopy was used, using a polyclonal antibody against human oviductin and Con A as a marker for mannose residues, to reveal the colocalization of human oviduct-specific glycoprotein with the endoplasmic reticulum and Golgi compartments. Setting: University-based anatomy and cell biology department. Patient(s): Women undergoing laparoscopy for tubal ligation or hysterectomy due to uterine fibroma. Intervention(s): An immortalized OE-E6/E7 cell line was previously established using human oviductal epithelial cells. Electron microscopy, RT-PCR, sequencing, immunohistochemistry and confocal microscopy were performed. Main Outcome Measure(s): The presence of human oviductin mRNA and protein in OE-E6/E7 cells. Result(s): OE-E6/E7 cells retain morphological features characteristic of secretory cells and express human oviductin mRNA and its translated protein. Conclusion(s): OE-E6/E7 cells were characterized for the first time by electron microscopy and shown to exhibit histological features typical of secretory cells. Reverse-transcription PCR with sequencing and confocal microscopy showed, respectively, that human oviductin mRNA and protein are expressed in OE-E6/E7 cells. Our results suggest that OE-E6/E7 could be a useful tool for future studies of the function of human oviductin. ©2005 by American Society for Reproductive Medicine.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.rightsFertility and Sterility. Copyright © Elsevier Inc.en_HK
dc.subjectConfocal microscopyen_HK
dc.subjectHuman oviductinen_HK
dc.subjectOE-E6/E7 cellsen_HK
dc.subjectOviduct-specific glycoproteinen_HK
dc.subjectOviductal cellsen_HK
dc.subjectOviductal secretionen_HK
dc.subjectRT-PCRen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshCell Line, Transformeden_HK
dc.subject.meshEpithelial Cells - metabolism - ultrastructureen_HK
dc.subject.meshFallopian Tubes - cytology - metabolism - ultrastructureen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulation - genetics - physiologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshRNA, Messenger - biosynthesis - geneticsen_HK
dc.subject.meshSerine Endopeptidases - biosynthesis - geneticsen_HK
dc.titleExpression of human oviductin in an immortalized human oviductal cell lineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0015-0282&volume=84 Supplement 2&spage=1095&epage=1103&date=2005&atitle=Expression+of+human+oviductin+in+an+immortalized+human+oviductal+cell+lineen_HK
dc.identifier.emailLee, YL:h9316321@hku.hken_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, YL=rp00308en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.fertnstert.2005.06.006en_HK
dc.identifier.pmid16209999-
dc.identifier.scopuseid_2-s2.0-25844481633en_HK
dc.identifier.hkuros109880en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-25844481633&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume84en_HK
dc.identifier.issueSUPPL. 2en_HK
dc.identifier.spage1095en_HK
dc.identifier.epage1103en_HK
dc.identifier.isiWOS:000232604700006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLing, L=36842991000en_HK
dc.identifier.scopusauthoridLee, YL=15033851800en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridKan, FWK=7004973274en_HK

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