File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Methylation-associated silencing of the Wnt antagonist SFRP1 gene in human ovarian cancers

TitleMethylation-associated silencing of the Wnt antagonist SFRP1 gene in human ovarian cancers
Authors
Issue Date2004
PublisherBlackwell Publishing Japan. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CAS
Citation
Cancer Science, 2004, v. 95 n. 9, p. 741-744 How to Cite?
AbstractThe SFRP1 gene on chromosome 8p11.2 encodes a Wnt signaling antagonist, and was recently demonstrated to be a new tumor suppressor that is inactivated by promoter methylation in human colon cancers. Here, we analyzed promoter methylation of the SFRP1 gene in human ovarian cancers, in which loss of heterozygosity in 8p is frequently observed and involvement of the Wnt signaling pathway has been suggested. Methylation-specific PCR (MSP) analysis showed that four of 13 ovarian cancer cell lines and two of 17 primary ovarian cancers had methylated SFRP1, while an immortalized ovarian epithelial cell line, HOSE, and seven ovarian endometrial cyst samples did not. In the four ovarian cancer cell lines with the methylation, SFRP1 was not expressed at all as determined by quantitative RT-PCR analysis. A cell line with SFRP1 methylation, MCAS, was treated with a demethylating agent, 5-aza-2′-deoxycytidine, and demethylation of the promoter and re-expression of SFRP1 were observed. These results show that SFRP1 is inactivated by promoter methylation in human ovarian cancers, as well as colon cancers.
Persistent Identifierhttp://hdl.handle.net/10722/67336
ISSN
2015 Impact Factor: 3.896
2015 SCImago Journal Rankings: 1.744
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTakada, Ten_HK
dc.contributor.authorYagi, Yen_HK
dc.contributor.authorMaekita, Ten_HK
dc.contributor.authorImura, Men_HK
dc.contributor.authorNakagawa, Sen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorMiyamoto, Ken_HK
dc.contributor.authorYoshino, Oen_HK
dc.contributor.authorYasugi, Ten_HK
dc.contributor.authorTaketani, Yen_HK
dc.contributor.authorUshijima, Ten_HK
dc.date.accessioned2010-09-06T05:54:08Z-
dc.date.available2010-09-06T05:54:08Z-
dc.date.issued2004en_HK
dc.identifier.citationCancer Science, 2004, v. 95 n. 9, p. 741-744en_HK
dc.identifier.issn1347-9032en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67336-
dc.description.abstractThe SFRP1 gene on chromosome 8p11.2 encodes a Wnt signaling antagonist, and was recently demonstrated to be a new tumor suppressor that is inactivated by promoter methylation in human colon cancers. Here, we analyzed promoter methylation of the SFRP1 gene in human ovarian cancers, in which loss of heterozygosity in 8p is frequently observed and involvement of the Wnt signaling pathway has been suggested. Methylation-specific PCR (MSP) analysis showed that four of 13 ovarian cancer cell lines and two of 17 primary ovarian cancers had methylated SFRP1, while an immortalized ovarian epithelial cell line, HOSE, and seven ovarian endometrial cyst samples did not. In the four ovarian cancer cell lines with the methylation, SFRP1 was not expressed at all as determined by quantitative RT-PCR analysis. A cell line with SFRP1 methylation, MCAS, was treated with a demethylating agent, 5-aza-2′-deoxycytidine, and demethylation of the promoter and re-expression of SFRP1 were observed. These results show that SFRP1 is inactivated by promoter methylation in human ovarian cancers, as well as colon cancers.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Japan. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CASen_HK
dc.relation.ispartofCancer Scienceen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAzacitidine - analogs & derivatives - pharmacologyen_HK
dc.subject.meshDNA Methylationen_HK
dc.subject.meshDNA Modification Methylases - antagonists & inhibitorsen_HK
dc.subject.meshEnzyme Inhibitors - pharmacologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshGene Silencingen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIntercellular Signaling Peptides and Proteins - geneticsen_HK
dc.subject.meshMembrane Proteins - geneticsen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshOvarian Neoplasms - genetics - metabolismen_HK
dc.subject.meshPromoter Regions, Genetic - geneticsen_HK
dc.subject.meshProto-Oncogene Proteins - antagonists & inhibitorsen_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshWnt Proteinsen_HK
dc.titleMethylation-associated silencing of the Wnt antagonist SFRP1 gene in human ovarian cancersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1347-9032&volume=95 no9&spage=741&epage=744&date=2004&atitle=Methylation-associated+silencing+of+the+Wnt+antagonist+SFRP1+gene+in+human+ovarian+cancersen_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1349-7006.2004.tb03255.xen_HK
dc.identifier.pmid15471560-
dc.identifier.scopuseid_2-s2.0-5044238640en_HK
dc.identifier.hkuros95037en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-5044238640&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume95en_HK
dc.identifier.issue9en_HK
dc.identifier.spage741en_HK
dc.identifier.epage744en_HK
dc.identifier.isiWOS:000224088400008-
dc.publisher.placeJapanen_HK
dc.identifier.scopusauthoridTakada, T=7202751924en_HK
dc.identifier.scopusauthoridYagi, Y=7202568347en_HK
dc.identifier.scopusauthoridMaekita, T=8433096400en_HK
dc.identifier.scopusauthoridImura, M=7004885133en_HK
dc.identifier.scopusauthoridNakagawa, S=7402703104en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridMiyamoto, K=36236877400en_HK
dc.identifier.scopusauthoridYoshino, O=35387286400en_HK
dc.identifier.scopusauthoridYasugi, T=7005542591en_HK
dc.identifier.scopusauthoridTaketani, Y=35375161400en_HK
dc.identifier.scopusauthoridUshijima, T=7102749551en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats