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Article: The potential use of desmopressin to correct hypothermia-induced impairment of primary haemostasis-An in vitro study using PFA-100®

TitleThe potential use of desmopressin to correct hypothermia-induced impairment of primary haemostasis-An in vitro study using PFA-100®
Authors
KeywordsDesmopressin
Hypothermia
PFA-100® platelet function
Primary haemostasis
Issue Date2008
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/resuscitation
Citation
Resuscitation, 2008, v. 76 n. 1, p. 129-133 How to Cite?
AbstractObjective: Mild hypothermia (32-35 °C) impairs primary haemostasis and coagulation. Correction of these haemostatic impairments by rewarming alone may not be possible or desirable, particularly in major trauma, neuroanaesthesia and in critically ill patients. Pharmacological treatment of these impairments, if available, may be a useful alternative. Desmopressin has been used to treat various congenital and acquired platelet disorders, but its effects on hypothermia-induced impairment of primary haemostasis is not known. This study aims to investigate the in vitro effects of desmopressin on hypothermia-induced impairment of primary haemostasis using PFA-100® platelet function analyzer. Methods: Whole blood was collected from 20 healthy volunteers, divided into 2.7 ml aliquots and some incubated at 32 °C, and others at 37 °C as control. Three log doses of desmopressin (0.01, 0.1 or 1 nM) were added to aliquots at 32 °C, and saline was added to controls at both 32 and 37 °C, all in 0.1 ml volume. After incubating for 30 min, closure times (CT) was measured by PFA-100® using both collagen/epinephrine (adrenaline) (Col/EPI) and collagen/adenosine-5′-diphosphate (Col/ADP) cartridges. Results: CT was prolonged by 30.9% (Col/EPI) and 18.8% (Col/ADP) at 32 °C, respectively, compared to 37 °C (P < 0.001). All the three doses of desmopressin significantly, but incompletely corrected CT prolongation due to hypothermia (P < 0.002). Conclusion: Desmopressin partially reverses hypothermia-induced impairment of primary haemostasis in vitro, and may be potentially useful in improving haemostasis in hypothermic patients with bleeding where immediate rewarming is difficult or undesirable. © 2007 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67318
ISSN
2015 Impact Factor: 5.414
2015 SCImago Journal Rankings: 3.231
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYing, CLAen_HK
dc.contributor.authorTsang, SFen_HK
dc.contributor.authorNg, KFJen_HK
dc.date.accessioned2010-09-06T05:53:55Z-
dc.date.available2010-09-06T05:53:55Z-
dc.date.issued2008en_HK
dc.identifier.citationResuscitation, 2008, v. 76 n. 1, p. 129-133en_HK
dc.identifier.issn0300-9572en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67318-
dc.description.abstractObjective: Mild hypothermia (32-35 °C) impairs primary haemostasis and coagulation. Correction of these haemostatic impairments by rewarming alone may not be possible or desirable, particularly in major trauma, neuroanaesthesia and in critically ill patients. Pharmacological treatment of these impairments, if available, may be a useful alternative. Desmopressin has been used to treat various congenital and acquired platelet disorders, but its effects on hypothermia-induced impairment of primary haemostasis is not known. This study aims to investigate the in vitro effects of desmopressin on hypothermia-induced impairment of primary haemostasis using PFA-100® platelet function analyzer. Methods: Whole blood was collected from 20 healthy volunteers, divided into 2.7 ml aliquots and some incubated at 32 °C, and others at 37 °C as control. Three log doses of desmopressin (0.01, 0.1 or 1 nM) were added to aliquots at 32 °C, and saline was added to controls at both 32 and 37 °C, all in 0.1 ml volume. After incubating for 30 min, closure times (CT) was measured by PFA-100® using both collagen/epinephrine (adrenaline) (Col/EPI) and collagen/adenosine-5′-diphosphate (Col/ADP) cartridges. Results: CT was prolonged by 30.9% (Col/EPI) and 18.8% (Col/ADP) at 32 °C, respectively, compared to 37 °C (P < 0.001). All the three doses of desmopressin significantly, but incompletely corrected CT prolongation due to hypothermia (P < 0.002). Conclusion: Desmopressin partially reverses hypothermia-induced impairment of primary haemostasis in vitro, and may be potentially useful in improving haemostasis in hypothermic patients with bleeding where immediate rewarming is difficult or undesirable. © 2007 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/resuscitationen_HK
dc.relation.ispartofResuscitationen_HK
dc.subjectDesmopressinen_HK
dc.subjectHypothermiaen_HK
dc.subjectPFA-100® platelet functionen_HK
dc.subjectPrimary haemostasisen_HK
dc.subject.meshDeamino Arginine Vasopressin - pharmacology-
dc.subject.meshHemostasis - drug effects-
dc.subject.meshHemostatics - pharmacology-
dc.subject.meshHypothermia, Induced - adverse effects-
dc.subject.meshPlatelet Function Tests - instrumentation-
dc.titleThe potential use of desmopressin to correct hypothermia-induced impairment of primary haemostasis-An in vitro study using PFA-100®en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-9572&volume=76&issue=1&spage=129&epage=133&date=2008&atitle=The+potential+use+of+desmopressin+to+correct+hypothermia-induced+impairment+of+primary+haemostasis:+an+in+vitro+study+using+PFA-100en_HK
dc.identifier.emailNg, KFJ:jkfng@hkucc.hku.hken_HK
dc.identifier.authorityNg, KFJ=rp00544en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.resuscitation.2007.07.009en_HK
dc.identifier.pmid17714852-
dc.identifier.scopuseid_2-s2.0-37049030370en_HK
dc.identifier.hkuros137273en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-37049030370&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume76en_HK
dc.identifier.issue1en_HK
dc.identifier.spage129en_HK
dc.identifier.epage133en_HK
dc.identifier.eissn1873-1570-
dc.identifier.isiWOS:000253204500022-
dc.publisher.placeIrelanden_HK

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