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Article: Genistein potentiates protein kinase A activity in porcine coronary artery

TitleGenistein potentiates protein kinase A activity in porcine coronary artery
Authors
KeywordscAMP
Genistein
Protein kinase A
Relaxation
Vascular smooth muscle
Issue Date2008
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-8177
Citation
Molecular And Cellular Biochemistry, 2008, v. 311 n. 1-2, p. 37-44 How to Cite?
AbstractSoy consumption is associated with a lower risk of atherosclerotic disease in the oriental population. Genistein is a soy isoflavone bearing estrogenic properties. Previous experiments in our laboratory demonstrated the potentiation of endothelium-independent relaxation of coronary artery by both estrogen and genistein. The potentiating effects of both estrogen and genistein were mediated through the cAMP-signaling pathway. We hypothesize that genistein could enhance protein kinase A (PKA) activity in porcine coronary artery smooth muscle, thereby offering a mechanism for the potentiation of vascular relaxation by genistein. In our study, a high concentration of genistein (10 -4.5 M) significantly increased PKA activity in porcine coronary artery rings. While genistein at 10 -5.5 M and forskolin at 10 -7 M had no effect on PKA activity, the combination of the two compounds at the prescribed concentrations caused a significant increase in PKA activity. The increase in PKA activity by genistein was abolished by SQ 22536 (adenylate cyclase blocker), but not by NF 449 (Gs protein blocker) or ICI 182780 (estrogen receptor antagonist). Our results suggest that the action of genistein is mediated via adenylate cyclase, but does not appear to involve Gs protein or ICI 182780-sensitive estrogen receptor. © Springer Science+Business Media, LLC. 2007.
Persistent Identifierhttp://hdl.handle.net/10722/67306
ISSN
2015 Impact Factor: 2.613
2015 SCImago Journal Rankings: 1.019
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNg, William WHen_HK
dc.contributor.authorKeung, Wen_HK
dc.contributor.authorXu, YCen_HK
dc.contributor.authorNg, KFJen_HK
dc.contributor.authorLeung, GPHen_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.contributor.authorChoy, PCen_HK
dc.contributor.authorMan, RYKen_HK
dc.date.accessioned2010-09-06T05:53:49Z-
dc.date.available2010-09-06T05:53:49Z-
dc.date.issued2008en_HK
dc.identifier.citationMolecular And Cellular Biochemistry, 2008, v. 311 n. 1-2, p. 37-44en_HK
dc.identifier.issn0300-8177en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67306-
dc.description.abstractSoy consumption is associated with a lower risk of atherosclerotic disease in the oriental population. Genistein is a soy isoflavone bearing estrogenic properties. Previous experiments in our laboratory demonstrated the potentiation of endothelium-independent relaxation of coronary artery by both estrogen and genistein. The potentiating effects of both estrogen and genistein were mediated through the cAMP-signaling pathway. We hypothesize that genistein could enhance protein kinase A (PKA) activity in porcine coronary artery smooth muscle, thereby offering a mechanism for the potentiation of vascular relaxation by genistein. In our study, a high concentration of genistein (10 -4.5 M) significantly increased PKA activity in porcine coronary artery rings. While genistein at 10 -5.5 M and forskolin at 10 -7 M had no effect on PKA activity, the combination of the two compounds at the prescribed concentrations caused a significant increase in PKA activity. The increase in PKA activity by genistein was abolished by SQ 22536 (adenylate cyclase blocker), but not by NF 449 (Gs protein blocker) or ICI 182780 (estrogen receptor antagonist). Our results suggest that the action of genistein is mediated via adenylate cyclase, but does not appear to involve Gs protein or ICI 182780-sensitive estrogen receptor. © Springer Science+Business Media, LLC. 2007.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0300-8177en_HK
dc.relation.ispartofMolecular and Cellular Biochemistryen_HK
dc.rightsThe original publication is available at www.springerlink.com-
dc.subjectcAMPen_HK
dc.subjectGenisteinen_HK
dc.subjectProtein kinase Aen_HK
dc.subjectRelaxationen_HK
dc.subjectVascular smooth muscleen_HK
dc.subject.meshAdenine - analogs and derivatives - metabolism - pharmacology-
dc.subject.meshCoronary Vessels - anatomy and histology - drug effects - metabolism-
dc.subject.meshGenistein - metabolism - pharmacology-
dc.subject.meshMuscle, Smooth, Vascular - drug effects - metabolism-
dc.subject.meshProtein Kinase Inhibitors - metabolism - pharmacology-
dc.titleGenistein potentiates protein kinase A activity in porcine coronary arteryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-8177&volume=311&issue=1-2&spage=37&epage=44&date=2008&atitle=Genistein+potentiates+protein+kinase+A+activity+in+porcine+coronary+arteryen_HK
dc.identifier.emailNg, KFJ: jkfng@hkucc.hku.hken_HK
dc.identifier.emailLeung, GPH: gphleung@hkucc.hku.hken_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.authorityNg, KFJ=rp00544en_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.identifier.authorityVanhoutte, PM=rp00238en_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11010-007-9691-3en_HK
dc.identifier.pmid18165926-
dc.identifier.scopuseid_2-s2.0-41349091455en_HK
dc.identifier.hkuros168516en_HK
dc.identifier.hkuros143132-
dc.identifier.hkuros214410-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-41349091455&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume311en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage37en_HK
dc.identifier.epage44en_HK
dc.identifier.isiWOS:000254204500005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNg, William WH=7401613562en_HK
dc.identifier.scopusauthoridKeung, W=19337708900en_HK
dc.identifier.scopusauthoridXu, YC=35106482400en_HK
dc.identifier.scopusauthoridNg, KFJ=13608809400en_HK
dc.identifier.scopusauthoridLeung, GPH=35963668200en_HK
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_HK
dc.identifier.scopusauthoridChoy, PC=7006633002en_HK
dc.identifier.scopusauthoridMan, RYK=7004986435en_HK

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