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Article: Endothelium-derived hyperpolarizing factor mediated relaxations in pig coronary arteries do not involve Gi/o proteins

TitleEndothelium-derived hyperpolarizing factor mediated relaxations in pig coronary arteries do not involve Gi/o proteins
Authors
KeywordsEndothelium-derived hyperpolarizing factor
Gi/o protein
Pertussis toxin
Pig coronary artery
Issue Date2008
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/aps/index.html
Citation
Acta Pharmacologica Sinica, 2008, v. 29 n. 12, p. 1419-1424 How to Cite?
AbstractAim: Endothelium-dependent relaxations to certain neurohumoral substances are mediated by pertussis toxin-sensitive Gi/o protein. Our experiments were designed to determine the role, if any, of pertussis toxin-sensitive G-proteins in relaxations attributed to endothelium-derived hyperpolarizing factor (EDHF). Methods: Pig coronary arterial rings with endothelia were suspended in organ chambers flled with Krebs-Ringer bicarbonate solution maintained at 37 °C and continuously aerated with 95%O2 and 5% CO2. Isometric tension was measured during contractions to prostaglandin F2α in the presence of indomethacin and NΩnitro-L-arginine methyl ester (L-NAME). Results: Thrombin, the thrombin receptor-activating peptide SFLLRN, bradykinin, substance P, and calcimycin produced dose-dependent relaxations. These relaxations were not inhibited by prior incubation with pertussis toxin, but were abolished upon the addition of charybdotoxin plus apamin. Relaxations to the α2-adrenergic agonist UK14304 and those to serotonin were abolished in the presence of indomethacin and L-NAME. Conclusion: Unlike nitric oxide-mediated relaxations, EDHF-mediated relaxations of pig coronary arteries do not involve pertussis toxin-sensitive pathways and are Gi/o protein independent.
Persistent Identifierhttp://hdl.handle.net/10722/67291
ISSN
2014 Impact Factor: 2.912
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong21374077
Funding Information:

This study is supported in part by a CRCG grant from the University of Hong Kong (No 21374077) and in part by departmental funds.

References

 

DC FieldValueLanguage
dc.contributor.authorNg, KFJen_HK
dc.contributor.authorLeung, SWSen_HK
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.date.accessioned2010-09-06T05:53:41Z-
dc.date.available2010-09-06T05:53:41Z-
dc.date.issued2008en_HK
dc.identifier.citationActa Pharmacologica Sinica, 2008, v. 29 n. 12, p. 1419-1424en_HK
dc.identifier.issn1671-4083en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67291-
dc.description.abstractAim: Endothelium-dependent relaxations to certain neurohumoral substances are mediated by pertussis toxin-sensitive Gi/o protein. Our experiments were designed to determine the role, if any, of pertussis toxin-sensitive G-proteins in relaxations attributed to endothelium-derived hyperpolarizing factor (EDHF). Methods: Pig coronary arterial rings with endothelia were suspended in organ chambers flled with Krebs-Ringer bicarbonate solution maintained at 37 °C and continuously aerated with 95%O2 and 5% CO2. Isometric tension was measured during contractions to prostaglandin F2α in the presence of indomethacin and NΩnitro-L-arginine methyl ester (L-NAME). Results: Thrombin, the thrombin receptor-activating peptide SFLLRN, bradykinin, substance P, and calcimycin produced dose-dependent relaxations. These relaxations were not inhibited by prior incubation with pertussis toxin, but were abolished upon the addition of charybdotoxin plus apamin. Relaxations to the α2-adrenergic agonist UK14304 and those to serotonin were abolished in the presence of indomethacin and L-NAME. Conclusion: Unlike nitric oxide-mediated relaxations, EDHF-mediated relaxations of pig coronary arteries do not involve pertussis toxin-sensitive pathways and are Gi/o protein independent.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/aps/index.htmlen_HK
dc.relation.ispartofActa Pharmacologica Sinicaen_HK
dc.subjectEndothelium-derived hyperpolarizing factoren_HK
dc.subjectGi/o proteinen_HK
dc.subjectPertussis toxinen_HK
dc.subjectPig coronary arteryen_HK
dc.subject.meshBiological Factors - pharmacology-
dc.subject.meshCoronary Vessels - drug effects - physiology-
dc.subject.meshEndothelium-Dependent Relaxing Factors - pharmacology-
dc.subject.meshGTP-Binding Protein alpha Subunits, Gi-Go - metabolism-
dc.subject.meshMuscle Relaxation - drug effects-
dc.titleEndothelium-derived hyperpolarizing factor mediated relaxations in pig coronary arteries do not involve Gi/o proteinsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1671-4083&volume=29&spage=1419&epage=1424&date=2008&atitle=Endothelium-derived+hyperpolarizing+factor+mediated+relaxations+in+pig+coronary+arteries+do+not+involve+Gi/o+proteinsen_HK
dc.identifier.emailNg, KFJ: jkfng@hkucc.hku.hken_HK
dc.identifier.emailLeung, SWS: swsleung@hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.authorityNg, KFJ=rp00544en_HK
dc.identifier.authorityLeung, SWS=rp00235en_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.identifier.authorityVanhoutte, PM=rp00238en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1111/j.1745-7254.2008.00905.xen_HK
dc.identifier.pmid19026160en_HK
dc.identifier.scopuseid_2-s2.0-64549097688en_HK
dc.identifier.hkuros168517en_HK
dc.identifier.hkuros156180-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-64549097688&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue12en_HK
dc.identifier.spage1419en_HK
dc.identifier.epage1424en_HK
dc.identifier.isiWOS:000261710300003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNg, KFJ=13608809400en_HK
dc.identifier.scopusauthoridLeung, SWS=24540419500en_HK
dc.identifier.scopusauthoridMan, RYK=7004986435en_HK
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_HK
dc.identifier.citeulike3804483-

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