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Article: Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva

TitleExpression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva
Authors
KeywordsCFTR
Gene expression
Gingival epithelium
Periodontal disease
Issue Date2010
PublisherPortland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm
Citation
Cell Biology International, 2010, v. 34 n. 2, p. 147-152 How to Cite?
AbstractCFTR (cystic fibrosis transmembrane conductance regulator) is a cAMP-activated chloride channel that regulates electrolyte and water transport. The present study investigated the expression and localization of CFTR in human gingiva and explored the possible association of CFTR with periodontal conditions. CFTR expression in gingival biopsies from five periodontally healthy subjects and ten subjects with chronic periodontitis and in the RHGE (reconstituted human gingival epithelia) was detected by immunohistochemistry, whereas its expression in gingival biopsies was analysed by immunofluorescence staining. CFTR mRNA was analysed by reverse transcription-PCR. CFTR mRNA was detected in human gingival epithelia and RHGE. CFTR protein was detected in gingival biopsies from both healthy subjects and individuals with periodontitis and in RHGE. In healthy subjects, CFTR expression was mainly confined to the granular and spinous layers of epithelia and localized on the cell membrane. In patients with periodontitis, CFTR was detected in all layers of epithelia and the underlying connective tissues. The mean CFTR expression levels in periodontitis patients were significantly higher than those in healthy subjects. The present study for the first time showed the expression and localization of CFTR in human gingival epithelia. Elevated CFTR expression in periodontitis subjects implies the possible involvement of CFTR in periodontal disease pathogenesis. Further study is warranted to confirm the present findings. © The Author(s) Journal compilation © 2010 Portland Press Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/67268
ISSN
2015 Impact Factor: 1.663
2015 SCImago Journal Rankings: 0.705
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
University of Hong KongCRCG 200802159001
CRCG 200707176095
Funding Information:

This study was supported by the Hong Kong Research Grants Council [grant number HKU 7518/05M] and University of Hong Kong [grant numbers CRCG 200802159001, CRCG 200707176095].

References
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DC FieldValueLanguage
dc.contributor.authorAjonuma, LCen_HK
dc.contributor.authorLu, Qen_HK
dc.contributor.authorCheung, BPen_HK
dc.contributor.authorLeung, WKen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorJin, Len_HK
dc.date.accessioned2010-09-06T05:53:24Z-
dc.date.available2010-09-06T05:53:24Z-
dc.date.issued2010en_HK
dc.identifier.citationCell Biology International, 2010, v. 34 n. 2, p. 147-152en_HK
dc.identifier.issn1065-6995en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67268-
dc.description.abstractCFTR (cystic fibrosis transmembrane conductance regulator) is a cAMP-activated chloride channel that regulates electrolyte and water transport. The present study investigated the expression and localization of CFTR in human gingiva and explored the possible association of CFTR with periodontal conditions. CFTR expression in gingival biopsies from five periodontally healthy subjects and ten subjects with chronic periodontitis and in the RHGE (reconstituted human gingival epithelia) was detected by immunohistochemistry, whereas its expression in gingival biopsies was analysed by immunofluorescence staining. CFTR mRNA was analysed by reverse transcription-PCR. CFTR mRNA was detected in human gingival epithelia and RHGE. CFTR protein was detected in gingival biopsies from both healthy subjects and individuals with periodontitis and in RHGE. In healthy subjects, CFTR expression was mainly confined to the granular and spinous layers of epithelia and localized on the cell membrane. In patients with periodontitis, CFTR was detected in all layers of epithelia and the underlying connective tissues. The mean CFTR expression levels in periodontitis patients were significantly higher than those in healthy subjects. The present study for the first time showed the expression and localization of CFTR in human gingival epithelia. Elevated CFTR expression in periodontitis subjects implies the possible involvement of CFTR in periodontal disease pathogenesis. Further study is warranted to confirm the present findings. © The Author(s) Journal compilation © 2010 Portland Press Ltd.en_HK
dc.languageengen_HK
dc.publisherPortland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htmen_HK
dc.relation.ispartofCell Biology Internationalen_HK
dc.subjectCFTRen_HK
dc.subjectGene expressionen_HK
dc.subjectGingival epitheliumen_HK
dc.subjectPeriodontal diseaseen_HK
dc.subject.meshBiopsy-
dc.subject.meshCystic Fibrosis Transmembrane Conductance Regulator - analysis - genetics - metabolism-
dc.subject.meshGingiva - metabolism - pathology-
dc.subject.meshImmunohistochemistry-
dc.subject.meshMicroscopy, Fluorescence-
dc.titleExpression and localization of cystic fibrosis transmembrane conductance regulator in human gingivaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1065-6995&volume=34&issue=2&spage=147&epage=152&date=2010&atitle=Expression+and+localization+of+cystic+fibrosis+transmembrane+conductance+regulator+in+human+gingivaen_HK
dc.identifier.emailAjonuma, LC: louisca@hkucc.hku.hken_HK
dc.identifier.emailLeung, WK: ewkleung@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_HK
dc.identifier.emailJin, L: ljjin@hkucc.hku.hken_HK
dc.identifier.authorityAjonuma, LC=rp00051en_HK
dc.identifier.authorityLeung, WK=rp00019en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authorityJin, L=rp00028en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1042/CBI20090019en_HK
dc.identifier.pmid20050827-
dc.identifier.scopuseid_2-s2.0-77955293656en_HK
dc.identifier.hkuros168607en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955293656&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume34en_HK
dc.identifier.issue2en_HK
dc.identifier.spage147en_HK
dc.identifier.epage152en_HK
dc.identifier.isiWOS:000277391500002-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectNovel molecular mechanisms of innate host defense - implications in periodontal health and disease-
dc.identifier.scopusauthoridAjonuma, LC=6602292557en_HK
dc.identifier.scopusauthoridLu, Q=36128876000en_HK
dc.identifier.scopusauthoridCheung, BP=7103294773en_HK
dc.identifier.scopusauthoridLeung, WK=25224691800en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridJin, L=7403328850en_HK

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