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Article: Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva
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TitleExpression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva
 
AuthorsAjonuma, LC1
Lu, Q1
Cheung, BP1
Leung, WK1
Samaranayake, LP1
Jin, L1
 
KeywordsCFTR
Gene expression
Gingival epithelium
Periodontal disease
 
Issue Date2010
 
PublisherPortland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm
 
CitationCell Biology International, 2010, v. 34 n. 2, p. 147-152 [How to Cite?]
DOI: http://dx.doi.org/10.1042/CBI20090019
 
AbstractCFTR (cystic fibrosis transmembrane conductance regulator) is a cAMP-activated chloride channel that regulates electrolyte and water transport. The present study investigated the expression and localization of CFTR in human gingiva and explored the possible association of CFTR with periodontal conditions. CFTR expression in gingival biopsies from five periodontally healthy subjects and ten subjects with chronic periodontitis and in the RHGE (reconstituted human gingival epithelia) was detected by immunohistochemistry, whereas its expression in gingival biopsies was analysed by immunofluorescence staining. CFTR mRNA was analysed by reverse transcription-PCR. CFTR mRNA was detected in human gingival epithelia and RHGE. CFTR protein was detected in gingival biopsies from both healthy subjects and individuals with periodontitis and in RHGE. In healthy subjects, CFTR expression was mainly confined to the granular and spinous layers of epithelia and localized on the cell membrane. In patients with periodontitis, CFTR was detected in all layers of epithelia and the underlying connective tissues. The mean CFTR expression levels in periodontitis patients were significantly higher than those in healthy subjects. The present study for the first time showed the expression and localization of CFTR in human gingival epithelia. Elevated CFTR expression in periodontitis subjects implies the possible involvement of CFTR in periodontal disease pathogenesis. Further study is warranted to confirm the present findings. © The Author(s) Journal compilation © 2010 Portland Press Ltd.
 
ISSN1065-6995
2012 Impact Factor: 1.64
2012 SCImago Journal Rankings: 0.599
 
DOIhttp://dx.doi.org/10.1042/CBI20090019
 
ISI Accession Number IDWOS:000277391500002
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
University of Hong KongCRCG 200802159001
CRCG 200707176095
Funding Information:

This study was supported by the Hong Kong Research Grants Council [grant number HKU 7518/05M] and University of Hong Kong [grant numbers CRCG 200802159001, CRCG 200707176095].

 
ReferencesReferences in Scopus
 
GrantsNovel molecular mechanisms of innate host defense - implications in periodontal health and disease
 
DC FieldValue
dc.contributor.authorAjonuma, LC
 
dc.contributor.authorLu, Q
 
dc.contributor.authorCheung, BP
 
dc.contributor.authorLeung, WK
 
dc.contributor.authorSamaranayake, LP
 
dc.contributor.authorJin, L
 
dc.date.accessioned2010-09-06T05:53:24Z
 
dc.date.available2010-09-06T05:53:24Z
 
dc.date.issued2010
 
dc.description.abstractCFTR (cystic fibrosis transmembrane conductance regulator) is a cAMP-activated chloride channel that regulates electrolyte and water transport. The present study investigated the expression and localization of CFTR in human gingiva and explored the possible association of CFTR with periodontal conditions. CFTR expression in gingival biopsies from five periodontally healthy subjects and ten subjects with chronic periodontitis and in the RHGE (reconstituted human gingival epithelia) was detected by immunohistochemistry, whereas its expression in gingival biopsies was analysed by immunofluorescence staining. CFTR mRNA was analysed by reverse transcription-PCR. CFTR mRNA was detected in human gingival epithelia and RHGE. CFTR protein was detected in gingival biopsies from both healthy subjects and individuals with periodontitis and in RHGE. In healthy subjects, CFTR expression was mainly confined to the granular and spinous layers of epithelia and localized on the cell membrane. In patients with periodontitis, CFTR was detected in all layers of epithelia and the underlying connective tissues. The mean CFTR expression levels in periodontitis patients were significantly higher than those in healthy subjects. The present study for the first time showed the expression and localization of CFTR in human gingival epithelia. Elevated CFTR expression in periodontitis subjects implies the possible involvement of CFTR in periodontal disease pathogenesis. Further study is warranted to confirm the present findings. © The Author(s) Journal compilation © 2010 Portland Press Ltd.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationCell Biology International, 2010, v. 34 n. 2, p. 147-152 [How to Cite?]
DOI: http://dx.doi.org/10.1042/CBI20090019
 
dc.identifier.doihttp://dx.doi.org/10.1042/CBI20090019
 
dc.identifier.epage152
 
dc.identifier.hkuros168607
 
dc.identifier.isiWOS:000277391500002
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
University of Hong KongCRCG 200802159001
CRCG 200707176095
Funding Information:

This study was supported by the Hong Kong Research Grants Council [grant number HKU 7518/05M] and University of Hong Kong [grant numbers CRCG 200802159001, CRCG 200707176095].

 
dc.identifier.issn1065-6995
2012 Impact Factor: 1.64
2012 SCImago Journal Rankings: 0.599
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmid20050827
 
dc.identifier.scopuseid_2-s2.0-77955293656
 
dc.identifier.spage147
 
dc.identifier.urihttp://hdl.handle.net/10722/67268
 
dc.identifier.volume34
 
dc.languageeng
 
dc.publisherPortland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofCell Biology International
 
dc.relation.projectNovel molecular mechanisms of innate host defense - implications in periodontal health and disease
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshBiopsy
 
dc.subject.meshCystic Fibrosis Transmembrane Conductance Regulator - analysis - genetics - metabolism
 
dc.subject.meshGingiva - metabolism - pathology
 
dc.subject.meshImmunohistochemistry
 
dc.subject.meshMicroscopy, Fluorescence
 
dc.subjectCFTR
 
dc.subjectGene expression
 
dc.subjectGingival epithelium
 
dc.subjectPeriodontal disease
 
dc.titleExpression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong