Article: Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva
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- Publisher Website: 10.1042/CBI20090019
- Scopus: eid_2-s2.0-77955293656
- PMID: 20050827
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| Title | Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva |
|---|---|
| Authors | Ajonuma, LC1 Lu, Q1 Cheung, BP1 Leung, WK1 Samaranayake, LP1 Jin, L1 |
| Keywords | CFTR Gene expression Gingival epithelium Periodontal disease |
| Issue Date | 2010 |
| Publisher | Portland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm |
| Citation | Cell Biology International, 2010, v. 34 n. 2, p. 147-152 [How to Cite?] DOI: http://dx.doi.org/10.1042/CBI20090019 |
| Abstract | CFTR (cystic fibrosis transmembrane conductance regulator) is a cAMP-activated chloride channel that regulates electrolyte and water transport. The present study investigated the expression and localization of CFTR in human gingiva and explored the possible association of CFTR with periodontal conditions. CFTR expression in gingival biopsies from five periodontally healthy subjects and ten subjects with chronic periodontitis and in the RHGE (reconstituted human gingival epithelia) was detected by immunohistochemistry, whereas its expression in gingival biopsies was analysed by immunofluorescence staining. CFTR mRNA was analysed by reverse transcription-PCR. CFTR mRNA was detected in human gingival epithelia and RHGE. CFTR protein was detected in gingival biopsies from both healthy subjects and individuals with periodontitis and in RHGE. In healthy subjects, CFTR expression was mainly confined to the granular and spinous layers of epithelia and localized on the cell membrane. In patients with periodontitis, CFTR was detected in all layers of epithelia and the underlying connective tissues. The mean CFTR expression levels in periodontitis patients were significantly higher than those in healthy subjects. The present study for the first time showed the expression and localization of CFTR in human gingival epithelia. Elevated CFTR expression in periodontitis subjects implies the possible involvement of CFTR in periodontal disease pathogenesis. Further study is warranted to confirm the present findings. © The Author(s) Journal compilation © 2010 Portland Press Ltd. |
| ISSN | 1065-6995 2011 Impact Factor: 1.482 2011 SCImago Journal Rankings: 0.166 |
| DOI | http://dx.doi.org/10.1042/CBI20090019 |
| References | References in Scopus |
| Grants | Novel molecular mechanisms of innate host defense - implications in periodontal health and disease Novel molecular mechanisms of innate host defense - implications in periodontal health and disease |
| dc.contributor.author | Ajonuma, LC | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Lu, Q | ||||||
| dc.contributor.author | Cheung, BP | ||||||
| dc.contributor.author | Leung, WK | ||||||
| dc.contributor.author | Samaranayake, LP | ||||||
| dc.contributor.author | Jin, L | ||||||
| dc.date.accessioned | 2010-09-06T05:53:24Z | ||||||
| dc.date.available | 2010-09-06T05:53:24Z | ||||||
| dc.date.issued | 2010 | ||||||
| dc.description.abstract | CFTR (cystic fibrosis transmembrane conductance regulator) is a cAMP-activated chloride channel that regulates electrolyte and water transport. The present study investigated the expression and localization of CFTR in human gingiva and explored the possible association of CFTR with periodontal conditions. CFTR expression in gingival biopsies from five periodontally healthy subjects and ten subjects with chronic periodontitis and in the RHGE (reconstituted human gingival epithelia) was detected by immunohistochemistry, whereas its expression in gingival biopsies was analysed by immunofluorescence staining. CFTR mRNA was analysed by reverse transcription-PCR. CFTR mRNA was detected in human gingival epithelia and RHGE. CFTR protein was detected in gingival biopsies from both healthy subjects and individuals with periodontitis and in RHGE. In healthy subjects, CFTR expression was mainly confined to the granular and spinous layers of epithelia and localized on the cell membrane. In patients with periodontitis, CFTR was detected in all layers of epithelia and the underlying connective tissues. The mean CFTR expression levels in periodontitis patients were significantly higher than those in healthy subjects. The present study for the first time showed the expression and localization of CFTR in human gingival epithelia. Elevated CFTR expression in periodontitis subjects implies the possible involvement of CFTR in periodontal disease pathogenesis. Further study is warranted to confirm the present findings. © The Author(s) Journal compilation © 2010 Portland Press Ltd. | ||||||
| dc.description.grant | Novel molecular mechanisms of innate host defense - implications in periodontal health and disease | ||||||
| dc.description.grant | Novel molecular mechanisms of innate host defense - implications in periodontal health and disease | ||||||
| dc.description.grantcode | 28079 | ||||||
| dc.description.grantcode | 29685 | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Cell Biology International, 2010, v. 34 n. 2, p. 147-152 [How to Cite?] DOI: http://dx.doi.org/10.1042/CBI20090019 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1042/CBI20090019 | ||||||
| dc.identifier.epage | 152 | ||||||
| dc.identifier.hkuros | 168607 | ||||||
| dc.identifier.isi | WOS:000277391500002
Funding Information: This study was supported by the Hong Kong Research Grants Council [grant number HKU 7518/05M] and University of Hong Kong [grant numbers CRCG 200802159001, CRCG 200707176095]. | ||||||
| dc.identifier.issn | 1065-6995 2011 Impact Factor: 1.482 2011 SCImago Journal Rankings: 0.166 | ||||||
| dc.identifier.issue | 2 | ||||||
| dc.identifier.openurl | | ||||||
| dc.identifier.pmid | 20050827 | ||||||
| dc.identifier.scopus | eid_2-s2.0-77955293656 | ||||||
| dc.identifier.spage | 147 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/67268 | ||||||
| dc.identifier.volume | 34 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Portland Press Ltd.. The Journal's web site is located at http://www.cellbiolint.org/cbi/default.htm | ||||||
| dc.publisher.place | United Kingdom | ||||||
| dc.relation.ispartof | Cell Biology International | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Biopsy | ||||||
| dc.subject.mesh | Cystic Fibrosis Transmembrane Conductance Regulator - analysis - genetics - metabolism | ||||||
| dc.subject.mesh | Gingiva - metabolism - pathology | ||||||
| dc.subject.mesh | Immunohistochemistry | ||||||
| dc.subject.mesh | Microscopy, Fluorescence | ||||||
| dc.subject | CFTR | ||||||
| dc.subject | Gene expression | ||||||
| dc.subject | Gingival epithelium | ||||||
| dc.subject | Periodontal disease | ||||||
| dc.title | Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva | ||||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong