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Article: Local expression of lipopolysaccharide-binding protein in human gingival tissues

TitleLocal expression of lipopolysaccharide-binding protein in human gingival tissues
Authors
Issue Date2004
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
Citation
Journal Of Periodontal Research, 2004, v. 39 n. 4, p. 242-248 How to Cite?
AbstractBackground: Lipopolysaccharide-binding protein (LBP) functions as a crucial molecule in innate host defense responses to bacterial challenge through neutralization of bacterial lipopolysaccharide (LPS) and activation of cellular responses. Objectives: This study was to investigate the expression profile and levels of LBP in gingival tissues and their associations with periodontal health and disease. Methods: Gingival biopsies were collected from 44 chronic periodontitis patients, including periodontal pocket tissues (PoTs) and the adjacent healthy gingival tissues (HT-Ps), as well as from 15 periodontally healthy subjects as controls (HT-Cs). The peptide and mRNA of LBP were detected by semi-quantitative immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR), respectively. Results: LBP peptide was detected in 90.9% of PoTs (20/22), 84.6% of HT-Ps (11/13) and all HT-Cs (7/7). The expression of LBP was mainly confined to the cytoplasm of granular and keratinized layers of gingival epithelium, spreading from the oral sulcular epithelium to oral epithelium with the expression density decreasing gradually from coronal to apical portion. LBP peptide was also found on endothelial surfaces and/or inside the lumens of blood vessels in connective tissues. The mean LBP expression levels in HT-Cs were significantly higher than those in HT-Ps and PoTs. LBP mRNA was detected in 55% of PoTs (11/20), 55% of HT-Ps (11/20) and 75% of HT-Cs (6/8). Conclusions: We for the first time found the expression of LBP peptide and mRNA in human gingival tissues. Local expression of LBP in gingival tissues might contribute to periodontal homeostasis. © Blackwell Munksgaard, 2004.
Persistent Identifierhttp://hdl.handle.net/10722/67265
ISSN
2015 Impact Factor: 2.474
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRen, Len_HK
dc.contributor.authorJin, Len_HK
dc.contributor.authorLeung, WKen_HK
dc.date.accessioned2010-09-06T05:53:22Z-
dc.date.available2010-09-06T05:53:22Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Periodontal Research, 2004, v. 39 n. 4, p. 242-248en_HK
dc.identifier.issn0022-3484en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67265-
dc.description.abstractBackground: Lipopolysaccharide-binding protein (LBP) functions as a crucial molecule in innate host defense responses to bacterial challenge through neutralization of bacterial lipopolysaccharide (LPS) and activation of cellular responses. Objectives: This study was to investigate the expression profile and levels of LBP in gingival tissues and their associations with periodontal health and disease. Methods: Gingival biopsies were collected from 44 chronic periodontitis patients, including periodontal pocket tissues (PoTs) and the adjacent healthy gingival tissues (HT-Ps), as well as from 15 periodontally healthy subjects as controls (HT-Cs). The peptide and mRNA of LBP were detected by semi-quantitative immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR), respectively. Results: LBP peptide was detected in 90.9% of PoTs (20/22), 84.6% of HT-Ps (11/13) and all HT-Cs (7/7). The expression of LBP was mainly confined to the cytoplasm of granular and keratinized layers of gingival epithelium, spreading from the oral sulcular epithelium to oral epithelium with the expression density decreasing gradually from coronal to apical portion. LBP peptide was also found on endothelial surfaces and/or inside the lumens of blood vessels in connective tissues. The mean LBP expression levels in HT-Cs were significantly higher than those in HT-Ps and PoTs. LBP mRNA was detected in 55% of PoTs (11/20), 55% of HT-Ps (11/20) and 75% of HT-Cs (6/8). Conclusions: We for the first time found the expression of LBP peptide and mRNA in human gingival tissues. Local expression of LBP in gingival tissues might contribute to periodontal homeostasis. © Blackwell Munksgaard, 2004.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1en_HK
dc.relation.ispartofJournal of Periodontal Researchen_HK
dc.subject.meshAcute-Phase Proteins - analysisen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshCarrier Proteins - analysisen_HK
dc.subject.meshChi-Square Distributionen_HK
dc.subject.meshConnective Tissue - pathologyen_HK
dc.subject.meshCytoplasm - ultrastructureen_HK
dc.subject.meshEndothelial Cells - pathologyen_HK
dc.subject.meshEndothelium, Vascular - pathologyen_HK
dc.subject.meshEpithelial Cells - pathologyen_HK
dc.subject.meshFollow-Up Studiesen_HK
dc.subject.meshGingiva - pathologyen_HK
dc.subject.meshGingival Hemorrhage - pathology - therapyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImage Processing, Computer-Assisteden_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshLipopolysaccharides - analysisen_HK
dc.subject.meshMembrane Glycoproteinsen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPeriodontal Pocket - pathology - therapyen_HK
dc.subject.meshPeriodontitis - pathology - therapyen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.titleLocal expression of lipopolysaccharide-binding protein in human gingival tissuesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3484&volume=39&spage=242&epage=248&date=2004&atitle=Local+expression+of+lipopolysaccharide-binding+protein+in+human+gingival+tissuesen_HK
dc.identifier.emailJin, L:ljjin@hkucc.hku.hken_HK
dc.identifier.emailLeung, WK:ewkleung@hkucc.hku.hken_HK
dc.identifier.authorityJin, L=rp00028en_HK
dc.identifier.authorityLeung, WK=rp00019en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-0765.2004.00732.xen_HK
dc.identifier.pmid15206917-
dc.identifier.scopuseid_2-s2.0-3242742800en_HK
dc.identifier.hkuros88872en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3242742800&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue4en_HK
dc.identifier.spage242en_HK
dc.identifier.epage248en_HK
dc.identifier.isiWOS:000222175200006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridRen, L=35216223200en_HK
dc.identifier.scopusauthoridJin, L=7403328850en_HK
dc.identifier.scopusauthoridLeung, WK=25224691800en_HK

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