File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Effect of quercetin on preosteoblasts and bone defects

TitleEffect of quercetin on preosteoblasts and bone defects
Authors
KeywordsBone repair
Flavonol
Phytoestrogen
Quercetin
Issue Date2008
PublisherBentham Open. The Journal's web site is located at http://www.bentham.org/open/toorthj/
Citation
The Open Orthopaedics Journal, 2008, v. 2, p. 27-32 How to Cite?
AbstractQuercetin is a flavonol, also a phytoestrogen, available commonly in onion and apple. Our laboratory investigated its effect on MC3T3-E1 cells' alkaline phosphatase activity in vitro and compared the amount of new bone produced by quercetin in collagen matrix to that produced by bone grafts and collagen matrix in vivo. Four bone defects, 5mm by 10mm were created in the parietal bone of 2 New Zealand White rabbits. In the experimental animal, 2 defects were grafted with quercetin solution mixed with collagen matrix. In the control animal, 2 defects were grafted with collagen matrix alone. Animals were killed on day 14 and the defects were dissected and prepared for histological qualitative assessment. Results showed that 10muM of quercetin increased alkaline phosphatase activity of MC3T3-E1 cells at 72 hours in vitro by 32%. In the experimental animal, there was new bone growing inside the bone defects. In conclusion, specific concentration of quercetin increased alkaline phosphatase activity of MC3T3-E1 cells in vitro and quercetin in collagen matrix has the effect of forming new bone across bone defects in vivo.
Persistent Identifierhttp://hdl.handle.net/10722/67249
ISSN
PubMed Central ID

 

DC FieldValueLanguage
dc.contributor.authorWong, RWKen_HK
dc.contributor.authorRabie, ABMen_HK
dc.date.accessioned2010-09-06T05:53:14Z-
dc.date.available2010-09-06T05:53:14Z-
dc.date.issued2008en_HK
dc.identifier.citationThe Open Orthopaedics Journal, 2008, v. 2, p. 27-32en_HK
dc.identifier.issn1874-3250en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67249-
dc.description.abstractQuercetin is a flavonol, also a phytoestrogen, available commonly in onion and apple. Our laboratory investigated its effect on MC3T3-E1 cells' alkaline phosphatase activity in vitro and compared the amount of new bone produced by quercetin in collagen matrix to that produced by bone grafts and collagen matrix in vivo. Four bone defects, 5mm by 10mm were created in the parietal bone of 2 New Zealand White rabbits. In the experimental animal, 2 defects were grafted with quercetin solution mixed with collagen matrix. In the control animal, 2 defects were grafted with collagen matrix alone. Animals were killed on day 14 and the defects were dissected and prepared for histological qualitative assessment. Results showed that 10muM of quercetin increased alkaline phosphatase activity of MC3T3-E1 cells at 72 hours in vitro by 32%. In the experimental animal, there was new bone growing inside the bone defects. In conclusion, specific concentration of quercetin increased alkaline phosphatase activity of MC3T3-E1 cells in vitro and quercetin in collagen matrix has the effect of forming new bone across bone defects in vivo.-
dc.languageengen_HK
dc.publisherBentham Open. The Journal's web site is located at http://www.bentham.org/open/toorthj/en_HK
dc.relation.ispartofThe Open Orthopaedics Journalen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectBone repair-
dc.subjectFlavonol-
dc.subjectPhytoestrogen-
dc.subjectQuercetin-
dc.titleEffect of quercetin on preosteoblasts and bone defectsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1874-3250&volume= 2&spage=27&epage=32&date=2008&atitle=Effect+of+Quercetin+on+Preosteoblasts+and+Bone+Defectsen_HK
dc.identifier.emailWong, RWK: fyoung@hku.hken_HK
dc.identifier.emailRabie, ABM: rabie@hku.hken_HK
dc.identifier.authorityWong, RWK=rp00038en_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.2174/1874325000802010027-
dc.identifier.pmid19461927-
dc.identifier.pmcidPMC2685048-
dc.identifier.hkuros140829en_HK
dc.identifier.volume2-
dc.identifier.spage27-
dc.identifier.epage32-
dc.publisher.placeNetherlands-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats