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Article: An ultrastructural and a cytochemical study of candidal invasion of reconstituted human oral epithelium
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TitleAn ultrastructural and a cytochemical study of candidal invasion of reconstituted human oral epithelium
 
AuthorsJayatilake, JAMS2 3
Samaranayake, YH3
Samaranayake, LP1 3
 
Issue Date2005
 
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPM
 
CitationJournal Of Oral Pathology And Medicine, 2005, v. 34 n. 4, p. 240-246 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0714.2005.00307.x
 
AbstractBACKGROUND: Opportunistic yeast, Candida albicans causes superficial and systemic mycoses in compromised patients. Adhesion to host tissues, morphogenesis and extracellular phospholipases (PL) are thought to contribute to its virulence. The nature of numerous host-parasite interactions at the invasive phase of oral candidiasis is not fully understood. Hence in this study, we explore the ultrastructural features of oral candidiasis using a tissue culture model based on reconstituted human oral epithelium (RHOE). METHODS: Reconstituted human oral epithelium (Skinethic Laboratory, Nice, France) was inoculated with C. albicans SC5314 and incubated up to 48 h. The infected tissue was harvested at 12, 24 and 48 h and examined using light, scanning (SEN) and transmission electron microscopy (TEM). Localized activity of PLs of C. albicans during tissue invasion was also examined using a cytochemical method. RESULTS: Over a period of 48 h C. albicans invaded the RHOE, and histological examination revealed characteristic hallmarks of pathological tissue invasion. Hyphal penetration into the superficial epithelium, particularly at cell junctions, together with features of cellular internalization of yeasts was noted. Phospholipase activity was visible at the tips of hyphae and initial sites of bud formation. Further, SEM studies revealed cavitations on the surface epithelial cells particularly pronounced at the sites of hyphal invasion. Hyphal invasion was seen both at cell surfaces and intercellular cell junctions of the epithelium, the latter resembling thigmotropic behaviour. CONCLUSIONS: Our findings confirm that multiple cellular interactions such as internalization, thigmotropism and extracellular PLs contribute to invasive candidiasis. The RHOE model, described here, appears to be a satisfactory model for the investigation of ultrastructural and histochemical features of invasive candidiasis in humans. © Blackwell Munksgaard 2005 · All rights reserved.
 
ISSN0904-2512
2013 Impact Factor: 1.870
 
DOIhttp://dx.doi.org/10.1111/j.1600-0714.2005.00307.x
 
ISI Accession Number IDWOS:000227389600009
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorJayatilake, JAMS
 
dc.contributor.authorSamaranayake, YH
 
dc.contributor.authorSamaranayake, LP
 
dc.date.accessioned2010-09-06T05:52:22Z
 
dc.date.available2010-09-06T05:52:22Z
 
dc.date.issued2005
 
dc.description.abstractBACKGROUND: Opportunistic yeast, Candida albicans causes superficial and systemic mycoses in compromised patients. Adhesion to host tissues, morphogenesis and extracellular phospholipases (PL) are thought to contribute to its virulence. The nature of numerous host-parasite interactions at the invasive phase of oral candidiasis is not fully understood. Hence in this study, we explore the ultrastructural features of oral candidiasis using a tissue culture model based on reconstituted human oral epithelium (RHOE). METHODS: Reconstituted human oral epithelium (Skinethic Laboratory, Nice, France) was inoculated with C. albicans SC5314 and incubated up to 48 h. The infected tissue was harvested at 12, 24 and 48 h and examined using light, scanning (SEN) and transmission electron microscopy (TEM). Localized activity of PLs of C. albicans during tissue invasion was also examined using a cytochemical method. RESULTS: Over a period of 48 h C. albicans invaded the RHOE, and histological examination revealed characteristic hallmarks of pathological tissue invasion. Hyphal penetration into the superficial epithelium, particularly at cell junctions, together with features of cellular internalization of yeasts was noted. Phospholipase activity was visible at the tips of hyphae and initial sites of bud formation. Further, SEM studies revealed cavitations on the surface epithelial cells particularly pronounced at the sites of hyphal invasion. Hyphal invasion was seen both at cell surfaces and intercellular cell junctions of the epithelium, the latter resembling thigmotropic behaviour. CONCLUSIONS: Our findings confirm that multiple cellular interactions such as internalization, thigmotropism and extracellular PLs contribute to invasive candidiasis. The RHOE model, described here, appears to be a satisfactory model for the investigation of ultrastructural and histochemical features of invasive candidiasis in humans. © Blackwell Munksgaard 2005 · All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Oral Pathology And Medicine, 2005, v. 34 n. 4, p. 240-246 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0714.2005.00307.x
 
dc.identifier.citeulike115988
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-0714.2005.00307.x
 
dc.identifier.epage246
 
dc.identifier.hkuros98177
 
dc.identifier.isiWOS:000227389600009
 
dc.identifier.issn0904-2512
2013 Impact Factor: 1.870
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid15752260
 
dc.identifier.scopuseid_2-s2.0-15944387419
 
dc.identifier.spage240
 
dc.identifier.urihttp://hdl.handle.net/10722/67149
 
dc.identifier.volume34
 
dc.languageeng
 
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPM
 
dc.publisher.placeDenmark
 
dc.relation.ispartofJournal of Oral Pathology and Medicine
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshCandida - enzymology - pathogenicity - physiology
 
dc.subject.meshCandidiasis, Oral - microbiology
 
dc.subject.meshEpithelial Cells - microbiology
 
dc.subject.meshEpithelium - microbiology
 
dc.subject.meshHistocytochemistry
 
dc.subject.meshHumans
 
dc.subject.meshIntercellular Junctions - microbiology
 
dc.subject.meshLysophospholipase - analysis
 
dc.subject.meshMicroscopy, Electron, Scanning
 
dc.subject.meshMicroscopy, Electron, Transmission
 
dc.subject.meshMouth Mucosa - microbiology
 
dc.subject.meshPhospholipases A - analysis
 
dc.subject.meshTime Factors
 
dc.subject.meshTissue Culture Techniques
 
dc.subject.meshVirulence
 
dc.titleAn ultrastructural and a cytochemical study of candidal invasion of reconstituted human oral epithelium
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. University of Peradeniya
  3. Prince Philip Dental Hospital