File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: An ultrastructural and a cytochemical study of candidal invasion of reconstituted human oral epithelium

TitleAn ultrastructural and a cytochemical study of candidal invasion of reconstituted human oral epithelium
Authors
Issue Date2005
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPM
Citation
Journal Of Oral Pathology And Medicine, 2005, v. 34 n. 4, p. 240-246 How to Cite?
AbstractBACKGROUND: Opportunistic yeast, Candida albicans causes superficial and systemic mycoses in compromised patients. Adhesion to host tissues, morphogenesis and extracellular phospholipases (PL) are thought to contribute to its virulence. The nature of numerous host-parasite interactions at the invasive phase of oral candidiasis is not fully understood. Hence in this study, we explore the ultrastructural features of oral candidiasis using a tissue culture model based on reconstituted human oral epithelium (RHOE). METHODS: Reconstituted human oral epithelium (Skinethic Laboratory, Nice, France) was inoculated with C. albicans SC5314 and incubated up to 48 h. The infected tissue was harvested at 12, 24 and 48 h and examined using light, scanning (SEN) and transmission electron microscopy (TEM). Localized activity of PLs of C. albicans during tissue invasion was also examined using a cytochemical method. RESULTS: Over a period of 48 h C. albicans invaded the RHOE, and histological examination revealed characteristic hallmarks of pathological tissue invasion. Hyphal penetration into the superficial epithelium, particularly at cell junctions, together with features of cellular internalization of yeasts was noted. Phospholipase activity was visible at the tips of hyphae and initial sites of bud formation. Further, SEM studies revealed cavitations on the surface epithelial cells particularly pronounced at the sites of hyphal invasion. Hyphal invasion was seen both at cell surfaces and intercellular cell junctions of the epithelium, the latter resembling thigmotropic behaviour. CONCLUSIONS: Our findings confirm that multiple cellular interactions such as internalization, thigmotropism and extracellular PLs contribute to invasive candidiasis. The RHOE model, described here, appears to be a satisfactory model for the investigation of ultrastructural and histochemical features of invasive candidiasis in humans. © Blackwell Munksgaard 2005 · All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67149
ISSN
2015 Impact Factor: 1.859
2015 SCImago Journal Rankings: 0.731
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJayatilake, JAMSen_HK
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:52:22Z-
dc.date.available2010-09-06T05:52:22Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Oral Pathology And Medicine, 2005, v. 34 n. 4, p. 240-246en_HK
dc.identifier.issn0904-2512en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67149-
dc.description.abstractBACKGROUND: Opportunistic yeast, Candida albicans causes superficial and systemic mycoses in compromised patients. Adhesion to host tissues, morphogenesis and extracellular phospholipases (PL) are thought to contribute to its virulence. The nature of numerous host-parasite interactions at the invasive phase of oral candidiasis is not fully understood. Hence in this study, we explore the ultrastructural features of oral candidiasis using a tissue culture model based on reconstituted human oral epithelium (RHOE). METHODS: Reconstituted human oral epithelium (Skinethic Laboratory, Nice, France) was inoculated with C. albicans SC5314 and incubated up to 48 h. The infected tissue was harvested at 12, 24 and 48 h and examined using light, scanning (SEN) and transmission electron microscopy (TEM). Localized activity of PLs of C. albicans during tissue invasion was also examined using a cytochemical method. RESULTS: Over a period of 48 h C. albicans invaded the RHOE, and histological examination revealed characteristic hallmarks of pathological tissue invasion. Hyphal penetration into the superficial epithelium, particularly at cell junctions, together with features of cellular internalization of yeasts was noted. Phospholipase activity was visible at the tips of hyphae and initial sites of bud formation. Further, SEM studies revealed cavitations on the surface epithelial cells particularly pronounced at the sites of hyphal invasion. Hyphal invasion was seen both at cell surfaces and intercellular cell junctions of the epithelium, the latter resembling thigmotropic behaviour. CONCLUSIONS: Our findings confirm that multiple cellular interactions such as internalization, thigmotropism and extracellular PLs contribute to invasive candidiasis. The RHOE model, described here, appears to be a satisfactory model for the investigation of ultrastructural and histochemical features of invasive candidiasis in humans. © Blackwell Munksgaard 2005 · All rights reserved.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPMen_HK
dc.relation.ispartofJournal of Oral Pathology and Medicineen_HK
dc.subject.meshCandida - enzymology - pathogenicity - physiologyen_HK
dc.subject.meshCandidiasis, Oral - microbiologyen_HK
dc.subject.meshEpithelial Cells - microbiologyen_HK
dc.subject.meshEpithelium - microbiologyen_HK
dc.subject.meshHistocytochemistryen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIntercellular Junctions - microbiologyen_HK
dc.subject.meshLysophospholipase - analysisen_HK
dc.subject.meshMicroscopy, Electron, Scanningen_HK
dc.subject.meshMicroscopy, Electron, Transmissionen_HK
dc.subject.meshMouth Mucosa - microbiologyen_HK
dc.subject.meshPhospholipases A - analysisen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshTissue Culture Techniquesen_HK
dc.subject.meshVirulenceen_HK
dc.titleAn ultrastructural and a cytochemical study of candidal invasion of reconstituted human oral epitheliumen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0904-2512&volume=34&spage=240&epage=246&date=2005&atitle=An+ultrastructural+and+a+cytochemical+study+of+candidal+invasion+of+reconstituted+human+oral+epitheliumen_HK
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-0714.2005.00307.xen_HK
dc.identifier.pmid15752260-
dc.identifier.scopuseid_2-s2.0-15944387419en_HK
dc.identifier.hkuros98177en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-15944387419&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume34en_HK
dc.identifier.issue4en_HK
dc.identifier.spage240en_HK
dc.identifier.epage246en_HK
dc.identifier.isiWOS:000227389600009-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridJayatilake, JAMS=8441671500en_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.citeulike115988-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats