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Article: Candida albicans biofilm formation is associated with increased anti-oxidative capacities

TitleCandida albicans biofilm formation is associated with increased anti-oxidative capacities
Authors
Keywords2-DE
Anti-oxidant activity
Biofilm
Biomarker
Candida
Issue Date2008
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
Citation
Proteomics, 2008, v. 8 n. 14, p. 2936-2947 How to Cite?
AbstractCandida albicans is a common, opportunistic, human fungal pathogen that causes a variety of mucosal and systemic afflictions. It exists in nature both in the biofilm or the sessile phase, as well as in the free-floating or the planktonic phase. Candida biofilms, in particular, display unique characteristics that confer survival advantages over their planktonic counterparts, such as their recalcitrance to common antifungals. The mechanisms underlying Candida biofilm formation and their attributes are poorly understood. In this study, we used a 2-DE-based approach to characterize the protein markers that are differentially expressed in Candida biofilms in comparison to their planktonic counterparts. Using tandem mass spectrometric analysis, we have identified a significant number of proteins including alkyl hydroperoxide reductase, thioredoxin peroxidase, and thioredoxin involved in oxidative stress defenses that are upregulated in the biofilm phase. These proteomic findings were further confirmed by real-time PCR and lucigenin-based chemiluminescence assays. In addition, we demonstrate that a drug target for the new antifungal agent echinocandin, is abundantly expressed and significantly upregulated in Candida biofilms. Taken together, these data imply that the biofilm mode, Candida, compared with their planktonic counterparts, exhibits traits that can sustain oxidative stress (anti-oxidants), and thereby exert resistance to commonly used antifungals. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.
Persistent Identifierhttp://hdl.handle.net/10722/67145
ISSN
2015 Impact Factor: 4.079
2015 SCImago Journal Rankings: 1.476
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSeneviratne, CJen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorJin, Len_HK
dc.contributor.authorAbiko, Yen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:52:20Z-
dc.date.available2010-09-06T05:52:20Z-
dc.date.issued2008en_HK
dc.identifier.citationProteomics, 2008, v. 8 n. 14, p. 2936-2947en_HK
dc.identifier.issn1615-9853en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67145-
dc.description.abstractCandida albicans is a common, opportunistic, human fungal pathogen that causes a variety of mucosal and systemic afflictions. It exists in nature both in the biofilm or the sessile phase, as well as in the free-floating or the planktonic phase. Candida biofilms, in particular, display unique characteristics that confer survival advantages over their planktonic counterparts, such as their recalcitrance to common antifungals. The mechanisms underlying Candida biofilm formation and their attributes are poorly understood. In this study, we used a 2-DE-based approach to characterize the protein markers that are differentially expressed in Candida biofilms in comparison to their planktonic counterparts. Using tandem mass spectrometric analysis, we have identified a significant number of proteins including alkyl hydroperoxide reductase, thioredoxin peroxidase, and thioredoxin involved in oxidative stress defenses that are upregulated in the biofilm phase. These proteomic findings were further confirmed by real-time PCR and lucigenin-based chemiluminescence assays. In addition, we demonstrate that a drug target for the new antifungal agent echinocandin, is abundantly expressed and significantly upregulated in Candida biofilms. Taken together, these data imply that the biofilm mode, Candida, compared with their planktonic counterparts, exhibits traits that can sustain oxidative stress (anti-oxidants), and thereby exert resistance to commonly used antifungals. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomicsen_HK
dc.relation.ispartofProteomicsen_HK
dc.subject2-DEen_HK
dc.subjectAnti-oxidant activityen_HK
dc.subjectBiofilmen_HK
dc.subjectBiomarkeren_HK
dc.subjectCandidaen_HK
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAntioxidants - physiology-
dc.subject.meshBiofilms-
dc.subject.meshCandida albicans - genetics - pathogenicity - ultrastructure-
dc.subject.meshDrug Resistance, Fungal - genetics-
dc.titleCandida albicans biofilm formation is associated with increased anti-oxidative capacitiesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1615-9853&volume=8&issue=14&spage=2936&epage=2947&date=2008&atitle=Candida+albicans+biofilm+formation+is+associated+with+increased+anti-oxidative+capacitiesen_HK
dc.identifier.emailSeneviratne, CJ: jaya@hku.hken_HK
dc.identifier.emailWang, Y: yuwanghk@hku.hken_HK
dc.identifier.emailJin, L: ljjin@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_HK
dc.identifier.authoritySeneviratne, CJ=rp01372en_HK
dc.identifier.authorityWang, Y=rp00239en_HK
dc.identifier.authorityJin, L=rp00028en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pmic.200701097en_HK
dc.identifier.pmid18655069en_HK
dc.identifier.scopuseid_2-s2.0-48949100522en_HK
dc.identifier.hkuros142472en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-48949100522&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume8en_HK
dc.identifier.issue14en_HK
dc.identifier.spage2936en_HK
dc.identifier.epage2947en_HK
dc.identifier.eissn1615-9861-
dc.identifier.isiWOS:000258117400017-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridSeneviratne, CJ=6701897753en_HK
dc.identifier.scopusauthoridWang, Y=34973733700en_HK
dc.identifier.scopusauthoridJin, L=7403328850en_HK
dc.identifier.scopusauthoridAbiko, Y=23491243700en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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