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Article: Molecular evaluation of residual endodontic microorganisms after instrumentation, irrigation and medication with either calcium hydroxide or Septomixine

TitleMolecular evaluation of residual endodontic microorganisms after instrumentation, irrigation and medication with either calcium hydroxide or Septomixine
Authors
KeywordsActinomycetes
Bacteria
Calcium hydroxide
Endodontic therapy
Framycetin sulfate
Oligonucleotide-DNA hybridization
Polymerase chain reaction
Issue Date2004
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1
Citation
Oral Diseases, 2004, v. 10 n. 6, p. 389-397 How to Cite?
AbstractBACKGROUND AND OBJECTIVE: The correct choice of antimicrobial agents as inter-appointment medicaments is as important as the instrumentation and irrigation to remove pathogens from infected root canals. Calcium hydroxide [Ca(OH) 2] and framycetin sulfate (Septomixine) are common endodontic medicaments. Therefore, we evaluated the efficacy of either calcium hydroxide or Septomixine in eliminating residual intra-canal bacteria, particularly Actinomyces spp., during inter-appointment interval in endodontic therapy using molecular methods. METHODS: A total of 31 single-rooted teeth with primary root canal infections were studied immediately after opening the canals and subsequently after instrumentation, irrigation with sterile saline and 1-week medication with either Ca(OH) 2 (n = 25) or Septomixine (n = 6). Whole bacterial genomic DNA was isolated directly from samples and PCR with universal primers performed to detect total intra-canal bacteria. The variable regions of 16S rDNA of bacteria were amplified and labeled with digoxigenin for further hybridization to detect Actinomyces spp. A total of seven oligonucleotide probes specific for A. bovis, A. gerencseriae, A. israelii, A. meyeri, catalase-negative A. naeslundii (genospecies 1 and 2), catalase-positive A. naeslundii genospecies 2 and A. odontolyticus were used to detect Actinomyces spp. in 22 of 31 medicated root canals [Ca(OH) 2: n = 17; Septomixine: n = 5]. RESULTS: The PCR results showed that 25 of 31 examined canals were positively detected with residual microorganisms after instrumentation, irrigation with sterile saline and I -week medication with either Ca(OH) 2 (n = 20) or Septomixine (n = 5). Thus, only six canals [Ca(OH) 2: n = 5, Septomixine: n = 1] were aseptic after treatment. Hybridization results showed higher detection frequency of both A. odontolyticus and A. gerencseriae after treatment. Significant correlation was found between exposed pulp before treatment and positive detection of Actinomyces spp., particularly A. odontolyticus on the second visit (P < 0.05). CONCLUSION: The conventional, 1-week medication of either Ca(OH) 2 or Septomixine in endodontic therapy may not effectively inhibit residual bacterial growth in all root canals during inter-appointment intervals. Further investigations using, for instance quantitative real-time PCR analyses, are required to substantiate the present findings.
Persistent Identifierhttp://hdl.handle.net/10722/67124
ISSN
2015 Impact Factor: 2.0
2015 SCImago Journal Rankings: 0.828
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTang, Gen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorYip, HKen_HK
dc.date.accessioned2010-09-06T05:52:10Z-
dc.date.available2010-09-06T05:52:10Z-
dc.date.issued2004en_HK
dc.identifier.citationOral Diseases, 2004, v. 10 n. 6, p. 389-397en_HK
dc.identifier.issn1354-523Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/67124-
dc.description.abstractBACKGROUND AND OBJECTIVE: The correct choice of antimicrobial agents as inter-appointment medicaments is as important as the instrumentation and irrigation to remove pathogens from infected root canals. Calcium hydroxide [Ca(OH) 2] and framycetin sulfate (Septomixine) are common endodontic medicaments. Therefore, we evaluated the efficacy of either calcium hydroxide or Septomixine in eliminating residual intra-canal bacteria, particularly Actinomyces spp., during inter-appointment interval in endodontic therapy using molecular methods. METHODS: A total of 31 single-rooted teeth with primary root canal infections were studied immediately after opening the canals and subsequently after instrumentation, irrigation with sterile saline and 1-week medication with either Ca(OH) 2 (n = 25) or Septomixine (n = 6). Whole bacterial genomic DNA was isolated directly from samples and PCR with universal primers performed to detect total intra-canal bacteria. The variable regions of 16S rDNA of bacteria were amplified and labeled with digoxigenin for further hybridization to detect Actinomyces spp. A total of seven oligonucleotide probes specific for A. bovis, A. gerencseriae, A. israelii, A. meyeri, catalase-negative A. naeslundii (genospecies 1 and 2), catalase-positive A. naeslundii genospecies 2 and A. odontolyticus were used to detect Actinomyces spp. in 22 of 31 medicated root canals [Ca(OH) 2: n = 17; Septomixine: n = 5]. RESULTS: The PCR results showed that 25 of 31 examined canals were positively detected with residual microorganisms after instrumentation, irrigation with sterile saline and I -week medication with either Ca(OH) 2 (n = 20) or Septomixine (n = 5). Thus, only six canals [Ca(OH) 2: n = 5, Septomixine: n = 1] were aseptic after treatment. Hybridization results showed higher detection frequency of both A. odontolyticus and A. gerencseriae after treatment. Significant correlation was found between exposed pulp before treatment and positive detection of Actinomyces spp., particularly A. odontolyticus on the second visit (P < 0.05). CONCLUSION: The conventional, 1-week medication of either Ca(OH) 2 or Septomixine in endodontic therapy may not effectively inhibit residual bacterial growth in all root canals during inter-appointment intervals. Further investigations using, for instance quantitative real-time PCR analyses, are required to substantiate the present findings.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1en_HK
dc.relation.ispartofOral Diseasesen_HK
dc.subjectActinomycetesen_HK
dc.subjectBacteriaen_HK
dc.subjectCalcium hydroxideen_HK
dc.subjectEndodontic therapyen_HK
dc.subjectFramycetin sulfateen_HK
dc.subjectOligonucleotide-DNA hybridizationen_HK
dc.subjectPolymerase chain reactionen_HK
dc.subject.meshActinomycosis - drug therapy - microbiologyen_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAnti-Bacterial Agents - therapeutic useen_HK
dc.subject.meshCalcium Hydroxide - therapeutic useen_HK
dc.subject.meshDental Pulp Cavity - drug effects - microbiologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFramycetin - therapeutic useen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshMolecular Diagnostic Techniques - methodsen_HK
dc.subject.meshOdds Ratioen_HK
dc.subject.meshOligonucleotide Probes - diagnostic useen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.titleMolecular evaluation of residual endodontic microorganisms after instrumentation, irrigation and medication with either calcium hydroxide or Septomixineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1354-523X&volume=10&spage=389&epage=397&date=2004&atitle=Molecular+evaluation+of+residual+endodontic+microorganisms+after+instrumentation,+irrigation+and+medication+with+either+calcium+hydroxide+or+Septomixineen_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_HK
dc.identifier.emailYip, HK: kevin.h.k.yip@hkusua.hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authorityYip, HK=rp00027en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1601-0825.2004.01015.xen_HK
dc.identifier.pmid15533217-
dc.identifier.scopuseid_2-s2.0-9444248150en_HK
dc.identifier.hkuros96482en_HK
dc.identifier.hkuros114040-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-9444248150&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume10en_HK
dc.identifier.issue6en_HK
dc.identifier.spage389en_HK
dc.identifier.epage397en_HK
dc.identifier.isiWOS:000225014700014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTang, G=36882453100en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridYip, HK=25423244900en_HK
dc.identifier.citeulike20066-

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