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Article: Chromosome length polymorphism in clinical isolates of Candida parapsilosis

TitleChromosome length polymorphism in clinical isolates of Candida parapsilosis
Authors
Issue Date1998
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMIS
Citation
APMIS, 1998, v. 106 n. 10, p. 941-946 How to Cite?
AbstractChromosome length polymorphism among 24 clinical isolates of Candida parapsilosis obtained from several human sources was analysed using pulsed- field gel electrophoresis. The isolates, from both superficial and deep infections, comprised a miscellaneous collection from the oral cavity, blood cultures, ear infections, wound secrete, a venous catheter and peritoneal dialysis fluid. Contour-clamped homogenous field electrophoresis using a hexagonal electrode was used for pulsed field gel electrophoresis. The chromosome numbers varied from seven to nine and their sizes ranged from 0.75 to 2.6 Mb. According to the electrophoretic karyotype patterns the 24 isolates could be divided into 9 profiles. However, the majority (18 isolates) fell into 3 groups comprising 7, 8 and 9 chromosomes, containing 5, 11, and 2 isolates, respectively. The remaining six isolates, all of which were from either an oral or another superficial Site of isolation, could be categorized into a further six groups. These data confirm previous observations on the genomic heterogeneity of clinical isolates of C. parapsilosis, and illustrate the possible commonality in strains from related clinical habitats.
Persistent Identifierhttp://hdl.handle.net/10722/67113
ISSN
2015 Impact Factor: 1.933
2015 SCImago Journal Rankings: 0.855
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFernando, PHPen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:52:04Z-
dc.date.available2010-09-06T05:52:04Z-
dc.date.issued1998en_HK
dc.identifier.citationAPMIS, 1998, v. 106 n. 10, p. 941-946en_HK
dc.identifier.issn0903-4641en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67113-
dc.description.abstractChromosome length polymorphism among 24 clinical isolates of Candida parapsilosis obtained from several human sources was analysed using pulsed- field gel electrophoresis. The isolates, from both superficial and deep infections, comprised a miscellaneous collection from the oral cavity, blood cultures, ear infections, wound secrete, a venous catheter and peritoneal dialysis fluid. Contour-clamped homogenous field electrophoresis using a hexagonal electrode was used for pulsed field gel electrophoresis. The chromosome numbers varied from seven to nine and their sizes ranged from 0.75 to 2.6 Mb. According to the electrophoretic karyotype patterns the 24 isolates could be divided into 9 profiles. However, the majority (18 isolates) fell into 3 groups comprising 7, 8 and 9 chromosomes, containing 5, 11, and 2 isolates, respectively. The remaining six isolates, all of which were from either an oral or another superficial Site of isolation, could be categorized into a further six groups. These data confirm previous observations on the genomic heterogeneity of clinical isolates of C. parapsilosis, and illustrate the possible commonality in strains from related clinical habitats.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMISen_HK
dc.relation.ispartofAPMISen_HK
dc.subject.meshCandida - genetics - isolation & purificationen_HK
dc.subject.meshCandidiasis - microbiologyen_HK
dc.subject.meshCandidiasis, Cutaneous - microbiologyen_HK
dc.subject.meshCandidiasis, Oral - microbiologyen_HK
dc.subject.meshChromosomes, Fungal - chemistryen_HK
dc.subject.meshDNA, Fungal - analysisen_HK
dc.subject.meshGenotypeen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKaryotypingen_HK
dc.subject.meshOtitis - microbiologyen_HK
dc.subject.meshPolymorphism, Genetic - geneticsen_HK
dc.titleChromosome length polymorphism in clinical isolates of Candida parapsilosisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0903-4641&volume=106&spage=941&epage=946&date=1998&atitle=Chromosome+length+polymorphism+in+clinical+isolates+of+Candida+parapsilosisen_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1699-0463.1998.tb00243.x-
dc.identifier.pmid9833695-
dc.identifier.scopuseid_2-s2.0-0031730707en_HK
dc.identifier.hkuros38958en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031730707&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume106en_HK
dc.identifier.issue10en_HK
dc.identifier.spage941en_HK
dc.identifier.epage946en_HK
dc.identifier.isiWOS:000076948600004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridFernando, PHP=7006430039en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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