File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Lipopolysaccharide-binding protein down-regulates the expression of interleukin-6 by human gingival fibroblast

TitleLipopolysaccharide-binding protein down-regulates the expression of interleukin-6 by human gingival fibroblast
Authors
Issue Date2005
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
Citation
Journal Of Periodontal Research, 2005, v. 40 n. 5, p. 407-416 How to Cite?
AbstractBackground: Lipopolysaccharide-binding protein (LBP) participates in the interaction of lipopolysacchaide (LPS) with CD14 to modulate the expression of cytokines. Human gingival fibroblast may actively participate in LPS-induced immuno-inflammatory responses through CD14, toll-like receptor (TLR) superfamily, MD-2 and related adaptive proteins, leading to the expression of cytokines. Objectives: The present in vitro study aimed to investigate the possible effect of LBP and E. coli LPS interaction on the expression of cellular LPS receptors and IL-6 by human gingival fibroblast. Methods: The mRNA expression of CD14, LBP, TLR-2, TLR-4, MD-2 and IL-6 in human gingival fibroblast explants was detected by reverse transcription-polymerase chain reaction (RT-PCR) in the presence or absence of E. coli LPS and recombinant human LBP (rhLBP), while IL-6 peptides were analyzed by ELISA and immunohistochemistry, respectively. Results: Human gingival fibroblast could constitutively express CD14, MD-2 and IL-6 mRNAs, but not TLR-2, TLR-4 and LBP mRNAs. E. coli LPS induced the messages expression of MD-2, TLR-2 and -4. The expression of both IL-6 message and peptide was up-regulated by E. coli LPS in a dose dependent manner. Whereas rhLBP could significantly down-regulate the expression of both mRNAs and peptides of CD14 and IL-6 but not MD-2 signals in the presence or absence of E. coli LPS. The up-regulated expression of TLR-2 and -4 by E. coli LPS no longer existed in the presence of rhLBP. Conclusions: This study suggests that LBP may down-regulate the expression of IL-6 by human gingival fibroblast. Further studies are warranted to clarify the molecular mechanisms of LBP in regulation of cytokine expression by host cells and to elaborate the relevant clinical implications. © Blackwell Munksgaard 2005.
Persistent Identifierhttp://hdl.handle.net/10722/67090
ISSN
2015 Impact Factor: 2.474
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRen, Len_HK
dc.contributor.authorWai, KLen_HK
dc.contributor.authorTing, WLen_HK
dc.contributor.authorJin, Len_HK
dc.date.accessioned2010-09-06T05:51:53Z-
dc.date.available2010-09-06T05:51:53Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Periodontal Research, 2005, v. 40 n. 5, p. 407-416en_HK
dc.identifier.issn0022-3484en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67090-
dc.description.abstractBackground: Lipopolysaccharide-binding protein (LBP) participates in the interaction of lipopolysacchaide (LPS) with CD14 to modulate the expression of cytokines. Human gingival fibroblast may actively participate in LPS-induced immuno-inflammatory responses through CD14, toll-like receptor (TLR) superfamily, MD-2 and related adaptive proteins, leading to the expression of cytokines. Objectives: The present in vitro study aimed to investigate the possible effect of LBP and E. coli LPS interaction on the expression of cellular LPS receptors and IL-6 by human gingival fibroblast. Methods: The mRNA expression of CD14, LBP, TLR-2, TLR-4, MD-2 and IL-6 in human gingival fibroblast explants was detected by reverse transcription-polymerase chain reaction (RT-PCR) in the presence or absence of E. coli LPS and recombinant human LBP (rhLBP), while IL-6 peptides were analyzed by ELISA and immunohistochemistry, respectively. Results: Human gingival fibroblast could constitutively express CD14, MD-2 and IL-6 mRNAs, but not TLR-2, TLR-4 and LBP mRNAs. E. coli LPS induced the messages expression of MD-2, TLR-2 and -4. The expression of both IL-6 message and peptide was up-regulated by E. coli LPS in a dose dependent manner. Whereas rhLBP could significantly down-regulate the expression of both mRNAs and peptides of CD14 and IL-6 but not MD-2 signals in the presence or absence of E. coli LPS. The up-regulated expression of TLR-2 and -4 by E. coli LPS no longer existed in the presence of rhLBP. Conclusions: This study suggests that LBP may down-regulate the expression of IL-6 by human gingival fibroblast. Further studies are warranted to clarify the molecular mechanisms of LBP in regulation of cytokine expression by host cells and to elaborate the relevant clinical implications. © Blackwell Munksgaard 2005.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1en_HK
dc.relation.ispartofJournal of Periodontal Researchen_HK
dc.subject.meshAcute-Phase Proteins - pharmacologyen_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshAntigens, CD14 - analysis - drug effectsen_HK
dc.subject.meshAntigens, Surface - analysis - drug effectsen_HK
dc.subject.meshCarrier Proteins - analysis - drug effects - pharmacologyen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCytokines - analysis - drug effectsen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshDown-Regulationen_HK
dc.subject.meshEscherichia colien_HK
dc.subject.meshFibroblasts - cytology - drug effectsen_HK
dc.subject.meshGene Expression Regulation, Bacterialen_HK
dc.subject.meshGingiva - cytology - drug effectsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInterleukin-6 - analysisen_HK
dc.subject.meshLipopolysaccharides - pharmacologyen_HK
dc.subject.meshLymphocyte Antigen 96en_HK
dc.subject.meshMembrane Glycoproteins - analysis - drug effects - pharmacologyen_HK
dc.subject.meshReceptors, Cell Surface - analysis - drug effectsen_HK
dc.subject.meshToll-Like Receptor 2en_HK
dc.subject.meshToll-Like Receptor 4en_HK
dc.subject.meshToll-Like Receptorsen_HK
dc.titleLipopolysaccharide-binding protein down-regulates the expression of interleukin-6 by human gingival fibroblasten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3484&volume=40&spage=407&epage=416&date=2005&atitle=Lipopolysaccharide-binding+protein+down-regulates+the+expression+of+interleukin-6+by+human+gingival+fibroblasten_HK
dc.identifier.emailWai, KL:ewkleung@hkucc.hku.hken_HK
dc.identifier.emailJin, L:ljjin@hkucc.hku.hken_HK
dc.identifier.authorityWai, KL=rp00019en_HK
dc.identifier.authorityJin, L=rp00028en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-0765.2005.00822.xen_HK
dc.identifier.pmid16105094-
dc.identifier.scopuseid_2-s2.0-24344454612en_HK
dc.identifier.hkuros109978en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-24344454612&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume40en_HK
dc.identifier.issue5en_HK
dc.identifier.spage407en_HK
dc.identifier.epage416en_HK
dc.identifier.isiWOS:000231145900007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridRen, L=35216223200en_HK
dc.identifier.scopusauthoridWai, KL=25224691800en_HK
dc.identifier.scopusauthoridTing, WL=8665802400en_HK
dc.identifier.scopusauthoridJin, L=7403328850en_HK
dc.identifier.citeulike278372-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats