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Article: Amplification-based nucleic acid scanning techniques to assess genetic polymorphism in Candida

TitleAmplification-based nucleic acid scanning techniques to assess genetic polymorphism in Candida
Authors
Issue Date2003
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/1040841x.asp
Citation
Critical Reviews In Microbiology, 2003, v. 29 n. 1, p. 1-24 How to Cite?
AbstractOpportunistic pathogen Candida causes common fungal infections that manifest both superficially and systemically, especially in compromised patients. Although C. albicans is by far the main etiological agent of candidosis, the frequency of isolation of other non-albicans species such as C. glabrata and C. krusei is increasing at an alarming rate. Therefore, the epidemiology, pathogenicity, and diagnosis of infections due to these organisms are of great importance. Of a variety of genotyping methods utilized for strain delineation of these Candida species, amplification-based techniques such as randomly amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), restriction digestion-mediated PCR (RFLP-PCR), and single-stranded conformational polymorphism (SSCP) and microsatellite PCR (interrepeat PCR, IR-PCR) are the most popular and widely used. In the last decade or so these techniques have helped unravel the clinical epidemiological features of pathogenicity, diversity, microevolution, and natural heterozygosity in Candida species. Here we review in detail the basic principles of RAPD, the nature of the primer and factors influencing its selection, and the limitations of RAPD assays as well as analysis and interpretation of banding profiles generated using the software programs. In addition, the principles of other RAPD-based amplification techniques (AFLP, RFLP-PCR, SSCP, and IR-PCR) and their application in molecular epidemiologic studies of Candida species in particular and other fungi in general are also reviewed. It is concluded that these methods have wide applicability in genotyping fungi, although they differ greatly in their resolution and have advantages and drawbacks depending on the task in question.
Persistent Identifierhttp://hdl.handle.net/10722/67054
ISSN
2015 Impact Factor: 8.192
2015 SCImago Journal Rankings: 1.970
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorDassanayake, RSen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:51:34Z-
dc.date.available2010-09-06T05:51:34Z-
dc.date.issued2003en_HK
dc.identifier.citationCritical Reviews In Microbiology, 2003, v. 29 n. 1, p. 1-24en_HK
dc.identifier.issn1040-841Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/67054-
dc.description.abstractOpportunistic pathogen Candida causes common fungal infections that manifest both superficially and systemically, especially in compromised patients. Although C. albicans is by far the main etiological agent of candidosis, the frequency of isolation of other non-albicans species such as C. glabrata and C. krusei is increasing at an alarming rate. Therefore, the epidemiology, pathogenicity, and diagnosis of infections due to these organisms are of great importance. Of a variety of genotyping methods utilized for strain delineation of these Candida species, amplification-based techniques such as randomly amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), restriction digestion-mediated PCR (RFLP-PCR), and single-stranded conformational polymorphism (SSCP) and microsatellite PCR (interrepeat PCR, IR-PCR) are the most popular and widely used. In the last decade or so these techniques have helped unravel the clinical epidemiological features of pathogenicity, diversity, microevolution, and natural heterozygosity in Candida species. Here we review in detail the basic principles of RAPD, the nature of the primer and factors influencing its selection, and the limitations of RAPD assays as well as analysis and interpretation of banding profiles generated using the software programs. In addition, the principles of other RAPD-based amplification techniques (AFLP, RFLP-PCR, SSCP, and IR-PCR) and their application in molecular epidemiologic studies of Candida species in particular and other fungi in general are also reviewed. It is concluded that these methods have wide applicability in genotyping fungi, although they differ greatly in their resolution and have advantages and drawbacks depending on the task in question.en_HK
dc.languageengen_HK
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/1040841x.aspen_HK
dc.relation.ispartofCritical Reviews in Microbiologyen_HK
dc.rightsCritical Reviews in Microbiology. Copyright © Informa Healthcare.en_HK
dc.subject.meshCandida - classification - geneticsen_HK
dc.subject.meshDNA Primersen_HK
dc.subject.meshMicrosatellite Repeats - geneticsen_HK
dc.subject.meshNucleic Acid Amplification Techniques - methodsen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshPolymorphism, Geneticen_HK
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_HK
dc.subject.meshPolymorphism, Single-Stranded Conformationalen_HK
dc.subject.meshRandom Amplified Polymorphic DNA Technique - methodsen_HK
dc.subject.meshSpecies Specificityen_HK
dc.titleAmplification-based nucleic acid scanning techniques to assess genetic polymorphism in Candidaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1040-841X&volume=29&spage=1&epage=24&date=2003&atitle=Amplification-Based+Nucleic+Acid+Scanning+Techniques+to+Assess+Genetic+polymorphism+in+Candidaen_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1080/713610402-
dc.identifier.pmid12638716-
dc.identifier.scopuseid_2-s2.0-0037282415en_HK
dc.identifier.hkuros75790en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037282415&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue1en_HK
dc.identifier.spage1en_HK
dc.identifier.epage24en_HK
dc.identifier.isiWOS:000181429600001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridDassanayake, RS=6603321318en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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