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Article: Relationship of changes in interleukin-8 levels and granulocyte elastase activity in gingival crevicular fluid to subgingival periodontopathogens following non-surgical periodontal therapy in subjects with chronic periodontitis

TitleRelationship of changes in interleukin-8 levels and granulocyte elastase activity in gingival crevicular fluid to subgingival periodontopathogens following non-surgical periodontal therapy in subjects with chronic periodontitis
Authors
KeywordsChronic periodontitis
Gingival crevicular fluid
Granulocyte elastase
Interleukin-8
Periodontopathogen
Issue Date2002
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
Citation
Journal Of Clinical Periodontology, 2002, v. 29 n. 7, p. 604-614 How to Cite?
AbstractObjectives: To determine the effect of scaling and root planing (SRP) on the interrelations of subgingival periodontopathogens and both interleukin-8 (IL-8) and granulocyte elastase activity in gingival crevicular fluid (GCF), and to assess their relations to the short-term treatment response in management of chronic periodontitis. Material and methods: GCF and subgingival plaque were collected from 16 subjects with untreated chronic periodontitis at baseline and 4 weeks after SRP. IL-8 levels were determined by ELISA. Granulocyte elastase activity was analyzed with a specific substrate, pGluProVal-pNA, and the maximal rate of elastase activity (MR-EA) was calculated. 5 DNA-probes were used to detect the presence of A. actinomycetemcomitans (A. a.), B. forsythus (B.f.), P. gingivalis (P.g.), P. intermedia (P.i.), and T. denticola (T.d.), with a sensitivity = 103 cells/paper point. Results: IL-8 and MR-EA levels in GCF decreased significantly after SRP (p < 0.001) with a corresponding reduction of total count of the species. Of the sites with probing depth (PD) ≥ 5.0 mm and co-infection by B.f., P.g., P.i. & T.d. at baseline, the sites without persistent co-infection of these species after SRP exhibited a significant reduction of IL-8 levels (p < 0.02), MR-EA levels (p < 0.02) and PD (p < 0.01). No such change was found in the sites where such a co-infection persisted. Moreover, reduction of IL-8 levels in those pocket sites was accompanied by a concomitant reduction of MR-EA (p < 0.02) and PD (p < 0.01), while no significant change in MR-EA levels and PD was noted in those pocket sites that exhibited an increase of IL-8 levels after SRP. At baseline, the former group of sites showed significantly higher IL-8 levels than the latter group of sites (p < 0.02). Conclusions: IL-8-related granulocyte elastase activity was related to the change in infection patterns of the target periodontopathogens following scaling and root planing. Varying initial IL-8 levels in GCF and a corresponding shifting change of granulocyte elastase activity in GCF may characterize the different short-term treatment responses. © Blackwell Munksgaard, 2002.
Persistent Identifierhttp://hdl.handle.net/10722/67046
ISSN
2015 Impact Factor: 3.915
2015 SCImago Journal Rankings: 1.848
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, LJen_HK
dc.contributor.authorLeung, WKen_HK
dc.contributor.authorCorbet, EFen_HK
dc.contributor.authorSöder, Ben_HK
dc.date.accessioned2010-09-06T05:51:30Z-
dc.date.available2010-09-06T05:51:30Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Clinical Periodontology, 2002, v. 29 n. 7, p. 604-614en_HK
dc.identifier.issn0303-6979en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67046-
dc.description.abstractObjectives: To determine the effect of scaling and root planing (SRP) on the interrelations of subgingival periodontopathogens and both interleukin-8 (IL-8) and granulocyte elastase activity in gingival crevicular fluid (GCF), and to assess their relations to the short-term treatment response in management of chronic periodontitis. Material and methods: GCF and subgingival plaque were collected from 16 subjects with untreated chronic periodontitis at baseline and 4 weeks after SRP. IL-8 levels were determined by ELISA. Granulocyte elastase activity was analyzed with a specific substrate, pGluProVal-pNA, and the maximal rate of elastase activity (MR-EA) was calculated. 5 DNA-probes were used to detect the presence of A. actinomycetemcomitans (A. a.), B. forsythus (B.f.), P. gingivalis (P.g.), P. intermedia (P.i.), and T. denticola (T.d.), with a sensitivity = 103 cells/paper point. Results: IL-8 and MR-EA levels in GCF decreased significantly after SRP (p < 0.001) with a corresponding reduction of total count of the species. Of the sites with probing depth (PD) ≥ 5.0 mm and co-infection by B.f., P.g., P.i. & T.d. at baseline, the sites without persistent co-infection of these species after SRP exhibited a significant reduction of IL-8 levels (p < 0.02), MR-EA levels (p < 0.02) and PD (p < 0.01). No such change was found in the sites where such a co-infection persisted. Moreover, reduction of IL-8 levels in those pocket sites was accompanied by a concomitant reduction of MR-EA (p < 0.02) and PD (p < 0.01), while no significant change in MR-EA levels and PD was noted in those pocket sites that exhibited an increase of IL-8 levels after SRP. At baseline, the former group of sites showed significantly higher IL-8 levels than the latter group of sites (p < 0.02). Conclusions: IL-8-related granulocyte elastase activity was related to the change in infection patterns of the target periodontopathogens following scaling and root planing. Varying initial IL-8 levels in GCF and a corresponding shifting change of granulocyte elastase activity in GCF may characterize the different short-term treatment responses. © Blackwell Munksgaard, 2002.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPEen_HK
dc.relation.ispartofJournal of Clinical Periodontologyen_HK
dc.rightsThe definitive version is available at www.blackwell-synergy.com-
dc.subjectChronic periodontitisen_HK
dc.subjectGingival crevicular fluiden_HK
dc.subjectGranulocyte elastaseen_HK
dc.subjectInterleukin-8en_HK
dc.subjectPeriodontopathogenen_HK
dc.subject.meshGingival Crevicular Fluid - chemistry-
dc.subject.meshGram-Negative Bacteria - growth and development-
dc.subject.meshInterleukin-8 - analysis-
dc.subject.meshLeukocyte Elastase - analysis-
dc.subject.meshPeriodontitis - microbiology - therapy-
dc.titleRelationship of changes in interleukin-8 levels and granulocyte elastase activity in gingival crevicular fluid to subgingival periodontopathogens following non-surgical periodontal therapy in subjects with chronic periodontitisen_HK
dc.typeArticleen_HK
dc.identifier.emailJin, LJ:ljjin@hkucc.hku.hken_HK
dc.identifier.emailLeung, WK:ewkleung@hkucc.hku.hken_HK
dc.identifier.emailCorbet, EF:efcorbet@hku.hken_HK
dc.identifier.authorityJin, LJ=rp00028en_HK
dc.identifier.authorityLeung, WK=rp00019en_HK
dc.identifier.authorityCorbet, EF=rp00005en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1034/j.1600-051X.2002.290704.xen_HK
dc.identifier.pmid12354085-
dc.identifier.scopuseid_2-s2.0-0036636007en_HK
dc.identifier.hkuros80319en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036636007&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue7en_HK
dc.identifier.spage604en_HK
dc.identifier.epage614en_HK
dc.identifier.isiWOS:000178240800004-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridJin, LJ=7403328850en_HK
dc.identifier.scopusauthoridLeung, WK=25224691800en_HK
dc.identifier.scopusauthoridCorbet, EF=35609873200en_HK
dc.identifier.scopusauthoridSöder, B=6701795462en_HK

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