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Article: Effect of tensile force on expression of PTHrP and thickness of hypertrophic zone in organ-cultured mouse spheno-occipital synchondroses

TitleEffect of tensile force on expression of PTHrP and thickness of hypertrophic zone in organ-cultured mouse spheno-occipital synchondroses
Authors
Issue Date2008
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2008, v. 53 n. 7, p. 690-699 How to Cite?
AbstractObjective: Responses of spheno-occipital synchondroses to direct tensile stress have not been identified before. This study was, therefore designed to evaluate expression of PTHrP, and thickness of hypertrophic zone in spheno-occipital synchondroses in response to such stress, using mouse in vitro model. Methods: Spheno-occipital synchondroses together with adjacent structures were excised from fifty-five 2-day-old mice that were randomly assigned to 6 control and 5 experimental groups for 5 experimental periods (n = 5). In the experimental groups, tensile force of 0.2 g was applied across the synchondroses, using helical springs. In 5 control groups, the springs were made inactive. Both groups were then cultured for 6, 24, 48, 72 h and 7 days. Another control group was cultured without any springs for 7 days to compare with natural growth of the synchondroses from a group of five 9-day-old mice. Alcian blue-PAS staining was used to study growth of the synchondroses; immunohistochemical staining to identify PTHrP and type X collagen expression. The area of PTHrP expression and thickness of hypertrophic zone, demarcated by type X collagen expression, were measured. Results: Quantitative analysis showed that PTHrP expression increased significantly at hour 24 of the force application in the experimental group (p < 0.05), then reduced from hour 24 to 72 with a significant drop from hour 24 to 48 (p < 0.01); and the thickness of hypertrophic zone significantly increased at hour 48 (p < 0.01). Conclusions: Our findings suggested that the growth of spheno-occipital synchondroses could be modified by tensile stress; and a light continuous force could enhance its growth, as evidenced by an increase in PTHrP expression and thickness of hypertrophic zone. © 2008 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/67041
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRukkulchon, BKen_HK
dc.contributor.authorWong, RWKen_HK
dc.date.accessioned2010-09-06T05:51:28Z-
dc.date.available2010-09-06T05:51:28Z-
dc.date.issued2008en_HK
dc.identifier.citationArchives Of Oral Biology, 2008, v. 53 n. 7, p. 690-699en_HK
dc.identifier.issn0003-9969en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67041-
dc.description.abstractObjective: Responses of spheno-occipital synchondroses to direct tensile stress have not been identified before. This study was, therefore designed to evaluate expression of PTHrP, and thickness of hypertrophic zone in spheno-occipital synchondroses in response to such stress, using mouse in vitro model. Methods: Spheno-occipital synchondroses together with adjacent structures were excised from fifty-five 2-day-old mice that were randomly assigned to 6 control and 5 experimental groups for 5 experimental periods (n = 5). In the experimental groups, tensile force of 0.2 g was applied across the synchondroses, using helical springs. In 5 control groups, the springs were made inactive. Both groups were then cultured for 6, 24, 48, 72 h and 7 days. Another control group was cultured without any springs for 7 days to compare with natural growth of the synchondroses from a group of five 9-day-old mice. Alcian blue-PAS staining was used to study growth of the synchondroses; immunohistochemical staining to identify PTHrP and type X collagen expression. The area of PTHrP expression and thickness of hypertrophic zone, demarcated by type X collagen expression, were measured. Results: Quantitative analysis showed that PTHrP expression increased significantly at hour 24 of the force application in the experimental group (p < 0.05), then reduced from hour 24 to 72 with a significant drop from hour 24 to 48 (p < 0.01); and the thickness of hypertrophic zone significantly increased at hour 48 (p < 0.01). Conclusions: Our findings suggested that the growth of spheno-occipital synchondroses could be modified by tensile stress; and a light continuous force could enhance its growth, as evidenced by an increase in PTHrP expression and thickness of hypertrophic zone. © 2008 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_HK
dc.relation.ispartofArchives of Oral Biologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Proliferationen_HK
dc.subject.meshChondrocytes - cytology - metabolismen_HK
dc.subject.meshChondrogenesis - physiologyen_HK
dc.subject.meshGene Expressionen_HK
dc.subject.meshHypertrophy - genetics - metabolismen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred BALB Cen_HK
dc.subject.meshOccipital Bone - anatomy & histology - growth & developmenten_HK
dc.subject.meshOrgan Culture Techniquesen_HK
dc.subject.meshOsteogenesis - physiologyen_HK
dc.subject.meshParathyroid Hormone-Related Protein - genetics - metabolismen_HK
dc.subject.meshSkull Base - anatomy & histology - growth & developmenten_HK
dc.subject.meshSphenoid Bone - anatomy & histology - growth & developmenten_HK
dc.subject.meshStress, Mechanicalen_HK
dc.subject.meshTensile Strength - physiologyen_HK
dc.subject.meshUp-Regulation - physiologyen_HK
dc.titleEffect of tensile force on expression of PTHrP and thickness of hypertrophic zone in organ-cultured mouse spheno-occipital synchondrosesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-9969&volume=53&spage=690&epage=699&date=2008&atitle=Effect+of+Tensile+Force+on+Expression+of+PTHrP+and+Thickness+of+Hypertrophic+Zone+in+Organ-cultured+Mouse+Spheno-occipital+Synchondrosesen_HK
dc.identifier.emailWong, RWK:fyoung@hkucc.hku.hken_HK
dc.identifier.authorityWong, RWK=rp00038en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.archoralbio.2008.02.004en_HK
dc.identifier.pmid18343352en_HK
dc.identifier.scopuseid_2-s2.0-43049159677en_HK
dc.identifier.hkuros141981en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-43049159677&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume53en_HK
dc.identifier.issue7en_HK
dc.identifier.spage690en_HK
dc.identifier.epage699en_HK
dc.identifier.eissn1879-1506-
dc.identifier.isiWOS:000256719600012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridRukkulchon, BK=23973585900en_HK
dc.identifier.scopusauthoridWong, RWK=7402127170en_HK

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