Article: Preconditioning with Porphyromonas gingivalis lipopolysaccharide may confer cardioprotection and improve recovery of the electrically induced intracellular calcium transient during ischemia and reperfusion

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TitlePreconditioning with Porphyromonas gingivalis lipopolysaccharide may confer cardioprotection and improve recovery of the electrically induced intracellular calcium transient during ischemia and reperfusion
AuthorsFan, MHM1
Wong, KL1
Wu, S1
Leung, WK1 2
Yam, WC1
Wong, TM1 2
KeywordsCardiac myocyte
Lipopolysaccharide
Myocardial reperfusion injury
Porphyromonas gingivalis
Issue Date2010
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
CitationJournal Of Periodontal Research, 2010, v. 45 n. 1, p. 100-108 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0765.2009.01206.x
AbstractBackground and Objective: Porphyromonas gingivalis lipopolysaccharide (LPS) is a ligand for cell surface toll-like receptors (TLR), TLR2 and TLR4 while stimulation of either leads to cardioprotection. We hypothesized that: (1) pretreatment with P. gingivalis LPS at appropriate concentrations would induce cardioprotection against injury induced by ischemia and reperfusion; and (2) P. gingivalis LPS pretreatment at cardioprotective concentrations may reduce Ca2+ overload, which is a precipitating cause of injury, and improve recovery of contractile function. Material and Methods: Male Sprague-Dawley rats were randomly selected to receive intraperitoneal saline or hot phenol-water-extracted P. gingivalis LPS at 0.2, 0.5, 1.0, 2.0 or 4.0 mg/kg 24 h before the experiment. The hearts were isolated and subjected to regional ischemia by coronary artery ligation followed by reperfusion. In isolated rat ventricular myocytes, the cytosolic Ca2+ level and the electrically induced intracellular calcium (E[Ca2+]i) transient, which reflects contractile function, were determined after pretreatment with a cardioprotective dose of P. gingivalis LPS. Results: Pretreatment with 0.5 mg/kg P. gingivalis LPS significantly reduced, while pretreatment with 1.0-4.0 mg/kg significantly increased infarct size. The Ca2+ overload induced by ischemia-reperfusion was attenuated in myocytes from rats pretreated with 0.5 mg/kg P. gingivalis LPS. Pretreated myocytes also showed an increased amplitude of the E[Ca2+]i transient, no prolongation of the time to reach the peak E[Ca2+]i transient and shorter 50% decay time during reperfusion. Conclusion: At a dosage of 0.5 mg/kg, P. gingivalis LPS confers cardioprotection against ischemia-reperfusion-induced injury and improved intracellular E[Ca2+]i transient recovery, hence improving myocyte contractile recovery. © 2009 John Wiley & Sons A/S.
ISSN0022-3484
2011 Impact Factor: 1.686
2011 SCImago Journal Rankings: 0.120
DOIhttp://dx.doi.org/10.1111/j.1600-0765.2009.01206.x
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorFan, MHM
dc.contributor.authorWong, KL
dc.contributor.authorWu, S
dc.contributor.authorLeung, WK
dc.contributor.authorYam, WC
dc.contributor.authorWong, TM
dc.date.accessioned2010-09-06T05:51:18Z
dc.date.available2010-09-06T05:51:18Z
dc.date.issued2010
dc.description.abstractBackground and Objective: Porphyromonas gingivalis lipopolysaccharide (LPS) is a ligand for cell surface toll-like receptors (TLR), TLR2 and TLR4 while stimulation of either leads to cardioprotection. We hypothesized that: (1) pretreatment with P. gingivalis LPS at appropriate concentrations would induce cardioprotection against injury induced by ischemia and reperfusion; and (2) P. gingivalis LPS pretreatment at cardioprotective concentrations may reduce Ca2+ overload, which is a precipitating cause of injury, and improve recovery of contractile function. Material and Methods: Male Sprague-Dawley rats were randomly selected to receive intraperitoneal saline or hot phenol-water-extracted P. gingivalis LPS at 0.2, 0.5, 1.0, 2.0 or 4.0 mg/kg 24 h before the experiment. The hearts were isolated and subjected to regional ischemia by coronary artery ligation followed by reperfusion. In isolated rat ventricular myocytes, the cytosolic Ca2+ level and the electrically induced intracellular calcium (E[Ca2+]i) transient, which reflects contractile function, were determined after pretreatment with a cardioprotective dose of P. gingivalis LPS. Results: Pretreatment with 0.5 mg/kg P. gingivalis LPS significantly reduced, while pretreatment with 1.0-4.0 mg/kg significantly increased infarct size. The Ca2+ overload induced by ischemia-reperfusion was attenuated in myocytes from rats pretreated with 0.5 mg/kg P. gingivalis LPS. Pretreated myocytes also showed an increased amplitude of the E[Ca2+]i transient, no prolongation of the time to reach the peak E[Ca2+]i transient and shorter 50% decay time during reperfusion. Conclusion: At a dosage of 0.5 mg/kg, P. gingivalis LPS confers cardioprotection against ischemia-reperfusion-induced injury and improved intracellular E[Ca2+]i transient recovery, hence improving myocyte contractile recovery. © 2009 John Wiley & Sons A/S.
dc.description.naturepostprint
dc.identifier.citationJournal Of Periodontal Research, 2010, v. 45 n. 1, p. 100-108 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0765.2009.01206.x
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-0765.2009.01206.x
dc.identifier.epage108
dc.identifier.hkuros168550
dc.identifier.isiWOS:000273667900014
Funding AgencyGrant Number
LCST (Holdings) Ltd
Funding Information:

The study was partly supported by a Cardiovascular Research Fund donated by LCST (Holdings) Ltd. We thank C. P. Mok for technical assistance, Joyce Y. Y. Yau for P. gingivalis culture, Becky B. K. Cheung for P. gingivalis LPS extraction and Trevor Lane for technical editing.

dc.identifier.issn0022-3484
2011 Impact Factor: 1.686
2011 SCImago Journal Rankings: 0.120
dc.identifier.issue1
dc.identifier.openurl
dc.identifier.pmid19602110
dc.identifier.scopuseid_2-s2.0-74549115179
dc.identifier.spage100
dc.identifier.urihttp://hdl.handle.net/10722/67022
dc.identifier.volume45
dc.languageeng
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
dc.publisher.placeUnited States
dc.relation.ispartofJournal of Periodontal Research
dc.relation.referencesReferences in Scopus
dc.rightsThe definitive version is available at www3.interscience.wiley.com
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.subject.meshCalcium Signaling - drug effects
dc.subject.meshCardiotonic Agents - administration and dosage - therapeutic use
dc.subject.meshIschemic Preconditioning, Myocardial - methods
dc.subject.meshLipopolysaccharides - administration and dosage - therapeutic use
dc.subject.meshPorphyromonas gingivalis
dc.subjectCardiac myocyte
dc.subjectLipopolysaccharide
dc.subjectMyocardial reperfusion injury
dc.subjectPorphyromonas gingivalis
dc.titlePreconditioning with Porphyromonas gingivalis lipopolysaccharide may confer cardioprotection and improve recovery of the electrically induced intracellular calcium transient during ischemia and reperfusion
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong