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Article: Preconditioning with Porphyromonas gingivalis lipopolysaccharide may confer cardioprotection and improve recovery of the electrically induced intracellular calcium transient during ischemia and reperfusion
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TitlePreconditioning with Porphyromonas gingivalis lipopolysaccharide may confer cardioprotection and improve recovery of the electrically induced intracellular calcium transient during ischemia and reperfusion
 
AuthorsFan, MHM1
Wong, KL1
Wu, S1
Leung, WK1 2
Yam, WC1
Wong, TM1 2
 
KeywordsCardiac myocyte
Lipopolysaccharide
Myocardial reperfusion injury
Porphyromonas gingivalis
 
Issue Date2010
 
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
 
CitationJournal Of Periodontal Research, 2010, v. 45 n. 1, p. 100-108 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0765.2009.01206.x
 
AbstractBackground and Objective: Porphyromonas gingivalis lipopolysaccharide (LPS) is a ligand for cell surface toll-like receptors (TLR), TLR2 and TLR4 while stimulation of either leads to cardioprotection. We hypothesized that: (1) pretreatment with P. gingivalis LPS at appropriate concentrations would induce cardioprotection against injury induced by ischemia and reperfusion; and (2) P. gingivalis LPS pretreatment at cardioprotective concentrations may reduce Ca2+ overload, which is a precipitating cause of injury, and improve recovery of contractile function. Material and Methods: Male Sprague-Dawley rats were randomly selected to receive intraperitoneal saline or hot phenol-water-extracted P. gingivalis LPS at 0.2, 0.5, 1.0, 2.0 or 4.0 mg/kg 24 h before the experiment. The hearts were isolated and subjected to regional ischemia by coronary artery ligation followed by reperfusion. In isolated rat ventricular myocytes, the cytosolic Ca2+ level and the electrically induced intracellular calcium (E[Ca2+]i) transient, which reflects contractile function, were determined after pretreatment with a cardioprotective dose of P. gingivalis LPS. Results: Pretreatment with 0.5 mg/kg P. gingivalis LPS significantly reduced, while pretreatment with 1.0-4.0 mg/kg significantly increased infarct size. The Ca2+ overload induced by ischemia-reperfusion was attenuated in myocytes from rats pretreated with 0.5 mg/kg P. gingivalis LPS. Pretreated myocytes also showed an increased amplitude of the E[Ca2+]i transient, no prolongation of the time to reach the peak E[Ca2+]i transient and shorter 50% decay time during reperfusion. Conclusion: At a dosage of 0.5 mg/kg, P. gingivalis LPS confers cardioprotection against ischemia-reperfusion-induced injury and improved intracellular E[Ca2+]i transient recovery, hence improving myocyte contractile recovery. © 2009 John Wiley & Sons A/S.
 
ISSN0022-3484
2013 Impact Factor: 2.215
 
DOIhttp://dx.doi.org/10.1111/j.1600-0765.2009.01206.x
 
ISI Accession Number IDWOS:000273667900014
Funding AgencyGrant Number
LCST (Holdings) Ltd
Funding Information:

The study was partly supported by a Cardiovascular Research Fund donated by LCST (Holdings) Ltd. We thank C. P. Mok for technical assistance, Joyce Y. Y. Yau for P. gingivalis culture, Becky B. K. Cheung for P. gingivalis LPS extraction and Trevor Lane for technical editing.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorFan, MHM
 
dc.contributor.authorWong, KL
 
dc.contributor.authorWu, S
 
dc.contributor.authorLeung, WK
 
dc.contributor.authorYam, WC
 
dc.contributor.authorWong, TM
 
dc.date.accessioned2010-09-06T05:51:18Z
 
dc.date.available2010-09-06T05:51:18Z
 
dc.date.issued2010
 
dc.description.abstractBackground and Objective: Porphyromonas gingivalis lipopolysaccharide (LPS) is a ligand for cell surface toll-like receptors (TLR), TLR2 and TLR4 while stimulation of either leads to cardioprotection. We hypothesized that: (1) pretreatment with P. gingivalis LPS at appropriate concentrations would induce cardioprotection against injury induced by ischemia and reperfusion; and (2) P. gingivalis LPS pretreatment at cardioprotective concentrations may reduce Ca2+ overload, which is a precipitating cause of injury, and improve recovery of contractile function. Material and Methods: Male Sprague-Dawley rats were randomly selected to receive intraperitoneal saline or hot phenol-water-extracted P. gingivalis LPS at 0.2, 0.5, 1.0, 2.0 or 4.0 mg/kg 24 h before the experiment. The hearts were isolated and subjected to regional ischemia by coronary artery ligation followed by reperfusion. In isolated rat ventricular myocytes, the cytosolic Ca2+ level and the electrically induced intracellular calcium (E[Ca2+]i) transient, which reflects contractile function, were determined after pretreatment with a cardioprotective dose of P. gingivalis LPS. Results: Pretreatment with 0.5 mg/kg P. gingivalis LPS significantly reduced, while pretreatment with 1.0-4.0 mg/kg significantly increased infarct size. The Ca2+ overload induced by ischemia-reperfusion was attenuated in myocytes from rats pretreated with 0.5 mg/kg P. gingivalis LPS. Pretreated myocytes also showed an increased amplitude of the E[Ca2+]i transient, no prolongation of the time to reach the peak E[Ca2+]i transient and shorter 50% decay time during reperfusion. Conclusion: At a dosage of 0.5 mg/kg, P. gingivalis LPS confers cardioprotection against ischemia-reperfusion-induced injury and improved intracellular E[Ca2+]i transient recovery, hence improving myocyte contractile recovery. © 2009 John Wiley & Sons A/S.
 
dc.description.naturepostprint
 
dc.identifier.citationJournal Of Periodontal Research, 2010, v. 45 n. 1, p. 100-108 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-0765.2009.01206.x
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-0765.2009.01206.x
 
dc.identifier.epage108
 
dc.identifier.hkuros168550
 
dc.identifier.isiWOS:000273667900014
Funding AgencyGrant Number
LCST (Holdings) Ltd
Funding Information:

The study was partly supported by a Cardiovascular Research Fund donated by LCST (Holdings) Ltd. We thank C. P. Mok for technical assistance, Joyce Y. Y. Yau for P. gingivalis culture, Becky B. K. Cheung for P. gingivalis LPS extraction and Trevor Lane for technical editing.

 
dc.identifier.issn0022-3484
2013 Impact Factor: 2.215
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid19602110
 
dc.identifier.scopuseid_2-s2.0-74549115179
 
dc.identifier.spage100
 
dc.identifier.urihttp://hdl.handle.net/10722/67022
 
dc.identifier.volume45
 
dc.languageeng
 
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Periodontal Research
 
dc.relation.referencesReferences in Scopus
 
dc.rightsThe definitive version is available at www3.interscience.wiley.com
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshCalcium Signaling - drug effects
 
dc.subject.meshCardiotonic Agents - administration and dosage - therapeutic use
 
dc.subject.meshIschemic Preconditioning, Myocardial - methods
 
dc.subject.meshLipopolysaccharides - administration and dosage - therapeutic use
 
dc.subject.meshPorphyromonas gingivalis
 
dc.subjectCardiac myocyte
 
dc.subjectLipopolysaccharide
 
dc.subjectMyocardial reperfusion injury
 
dc.subjectPorphyromonas gingivalis
 
dc.titlePreconditioning with Porphyromonas gingivalis lipopolysaccharide may confer cardioprotection and improve recovery of the electrically induced intracellular calcium transient during ischemia and reperfusion
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong