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Article: Preconditioning with Porphyromonas gingivalis lipopolysaccharide may confer cardioprotection and improve recovery of the electrically induced intracellular calcium transient during ischemia and reperfusion

TitlePreconditioning with Porphyromonas gingivalis lipopolysaccharide may confer cardioprotection and improve recovery of the electrically induced intracellular calcium transient during ischemia and reperfusion
Authors
KeywordsCardiac myocyte
Lipopolysaccharide
Myocardial reperfusion injury
Porphyromonas gingivalis
Issue Date2010
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
Citation
Journal Of Periodontal Research, 2010, v. 45 n. 1, p. 100-108 How to Cite?
Abstract
Background and Objective: Porphyromonas gingivalis lipopolysaccharide (LPS) is a ligand for cell surface toll-like receptors (TLR), TLR2 and TLR4 while stimulation of either leads to cardioprotection. We hypothesized that: (1) pretreatment with P. gingivalis LPS at appropriate concentrations would induce cardioprotection against injury induced by ischemia and reperfusion; and (2) P. gingivalis LPS pretreatment at cardioprotective concentrations may reduce Ca2+ overload, which is a precipitating cause of injury, and improve recovery of contractile function. Material and Methods: Male Sprague-Dawley rats were randomly selected to receive intraperitoneal saline or hot phenol-water-extracted P. gingivalis LPS at 0.2, 0.5, 1.0, 2.0 or 4.0 mg/kg 24 h before the experiment. The hearts were isolated and subjected to regional ischemia by coronary artery ligation followed by reperfusion. In isolated rat ventricular myocytes, the cytosolic Ca2+ level and the electrically induced intracellular calcium (E[Ca2+]i) transient, which reflects contractile function, were determined after pretreatment with a cardioprotective dose of P. gingivalis LPS. Results: Pretreatment with 0.5 mg/kg P. gingivalis LPS significantly reduced, while pretreatment with 1.0-4.0 mg/kg significantly increased infarct size. The Ca2+ overload induced by ischemia-reperfusion was attenuated in myocytes from rats pretreated with 0.5 mg/kg P. gingivalis LPS. Pretreated myocytes also showed an increased amplitude of the E[Ca2+]i transient, no prolongation of the time to reach the peak E[Ca2+]i transient and shorter 50% decay time during reperfusion. Conclusion: At a dosage of 0.5 mg/kg, P. gingivalis LPS confers cardioprotection against ischemia-reperfusion-induced injury and improved intracellular E[Ca2+]i transient recovery, hence improving myocyte contractile recovery. © 2009 John Wiley & Sons A/S.
Persistent Identifierhttp://hdl.handle.net/10722/67022
ISSN
2013 Impact Factor: 2.215
ISI Accession Number ID
Funding AgencyGrant Number
LCST (Holdings) Ltd
Funding Information:

The study was partly supported by a Cardiovascular Research Fund donated by LCST (Holdings) Ltd. We thank C. P. Mok for technical assistance, Joyce Y. Y. Yau for P. gingivalis culture, Becky B. K. Cheung for P. gingivalis LPS extraction and Trevor Lane for technical editing.

References

 

Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorFan, MHMen_HK
dc.contributor.authorWong, KLen_HK
dc.contributor.authorWu, Sen_HK
dc.contributor.authorLeung, WKen_HK
dc.contributor.authorYam, WCen_HK
dc.contributor.authorWong, TMen_HK
dc.date.accessioned2010-09-06T05:51:18Z-
dc.date.available2010-09-06T05:51:18Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Periodontal Research, 2010, v. 45 n. 1, p. 100-108en_HK
dc.identifier.issn0022-3484en_HK
dc.identifier.urihttp://hdl.handle.net/10722/67022-
dc.description.abstractBackground and Objective: Porphyromonas gingivalis lipopolysaccharide (LPS) is a ligand for cell surface toll-like receptors (TLR), TLR2 and TLR4 while stimulation of either leads to cardioprotection. We hypothesized that: (1) pretreatment with P. gingivalis LPS at appropriate concentrations would induce cardioprotection against injury induced by ischemia and reperfusion; and (2) P. gingivalis LPS pretreatment at cardioprotective concentrations may reduce Ca2+ overload, which is a precipitating cause of injury, and improve recovery of contractile function. Material and Methods: Male Sprague-Dawley rats were randomly selected to receive intraperitoneal saline or hot phenol-water-extracted P. gingivalis LPS at 0.2, 0.5, 1.0, 2.0 or 4.0 mg/kg 24 h before the experiment. The hearts were isolated and subjected to regional ischemia by coronary artery ligation followed by reperfusion. In isolated rat ventricular myocytes, the cytosolic Ca2+ level and the electrically induced intracellular calcium (E[Ca2+]i) transient, which reflects contractile function, were determined after pretreatment with a cardioprotective dose of P. gingivalis LPS. Results: Pretreatment with 0.5 mg/kg P. gingivalis LPS significantly reduced, while pretreatment with 1.0-4.0 mg/kg significantly increased infarct size. The Ca2+ overload induced by ischemia-reperfusion was attenuated in myocytes from rats pretreated with 0.5 mg/kg P. gingivalis LPS. Pretreated myocytes also showed an increased amplitude of the E[Ca2+]i transient, no prolongation of the time to reach the peak E[Ca2+]i transient and shorter 50% decay time during reperfusion. Conclusion: At a dosage of 0.5 mg/kg, P. gingivalis LPS confers cardioprotection against ischemia-reperfusion-induced injury and improved intracellular E[Ca2+]i transient recovery, hence improving myocyte contractile recovery. © 2009 John Wiley & Sons A/S.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1en_HK
dc.relation.ispartofJournal of Periodontal Researchen_HK
dc.rightsThe definitive version is available at www3.interscience.wiley.com-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCardiac myocyteen_HK
dc.subjectLipopolysaccharideen_HK
dc.subjectMyocardial reperfusion injuryen_HK
dc.subjectPorphyromonas gingivalisen_HK
dc.subject.meshCalcium Signaling - drug effects-
dc.subject.meshCardiotonic Agents - administration and dosage - therapeutic use-
dc.subject.meshIschemic Preconditioning, Myocardial - methods-
dc.subject.meshLipopolysaccharides - administration and dosage - therapeutic use-
dc.subject.meshPorphyromonas gingivalis-
dc.titlePreconditioning with Porphyromonas gingivalis lipopolysaccharide may confer cardioprotection and improve recovery of the electrically induced intracellular calcium transient during ischemia and reperfusionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3484&volume=45&issue=1&spage=100&epage=108&date=2010&atitle=Preconditioning+with+Porphyromonas+gingivalis++lipopolysaccharide+may+confer+cardioprotection+and+improve+recovery+of+the+electrically+induced+intracellular+calcium+transient+during+ischemia+and+reperfusionen_HK
dc.identifier.emailLeung, WK:ewkleung@hkucc.hku.hken_HK
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_HK
dc.identifier.authorityLeung, WK=rp00019en_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1111/j.1600-0765.2009.01206.xen_HK
dc.identifier.pmid19602110en_HK
dc.identifier.scopuseid_2-s2.0-74549115179en_HK
dc.identifier.hkuros168550en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-74549115179&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue1en_HK
dc.identifier.spage100en_HK
dc.identifier.epage108en_HK
dc.identifier.isiWOS:000273667900014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridFan, MHM=36460814500en_HK
dc.identifier.scopusauthoridWong, KL=36462221700en_HK
dc.identifier.scopusauthoridWu, S=7408443898en_HK
dc.identifier.scopusauthoridLeung, WK=25224691800en_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK
dc.identifier.scopusauthoridWong, TM=7403531434en_HK

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