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Article: Human periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitro

TitleHuman periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitro
Authors
KeywordsHuman periodontal ligament cell
Mechanical force
Osteoblast
Issue Date2006
PublisherFrontiers in Bioscience. The Journal's web site is located at http://www.frontbiosci.org/
Citation
Frontiers In Bioscience, 2006, v. 11 n. 1 P.447-888, p. 776-781 How to Cite?
AbstractMechanical strain applied to bone leads to bone remodeling. In the oral cavity, it is unclear how such mechanical force applied to move teeth orthodontically induces alveolar bone remodeling. It is known that osteoclasts are the only cells that are responsible for bone resorption, while the formation and activity of osteoclasts are regulated by osteoblasts. So it is believed that osteoblasts play an important role not only in bone formation but in bone remodeling as well. Therefore, the purpose of this study was to examine the effect of mechanical force on human periodontal ligament (PDL) cells and whether they express osteoblastic characters in vitro. Methods: Human PDL cells cultured in vitro were loaded with intermittently stretching force for 24 hours. The expression of alkaline phosphatase (ALP), osteocalcin (OCN) and osteoprotegerin (OPG) were detected at mRNA and protein levels at 0, 2nd, 4th, 6th, 12th, 24th hours after intermittent force loading. Results: Without any stimulation, ALP and OPG mRNA expressions were detected in human PDL cells by in-situ hybridization, but not that of OCN mRNA. ALP mRNA signal was up-regulated and that of OPG was down-regulated by mechanical force within 24 hours. OCN mRNA expression was induced by mechanical force in the late phase of the 24-hours loading cycle. The changes in secreted proteins showed similar results with those seen at the mRNA level. Conclusion: Human PDL cells express osteoblastic phenotypes under intermittent force loading and play a role in alveolar bone remodeling.
Persistent Identifierhttp://hdl.handle.net/10722/66910
ISSN
2015 Impact Factor: 2.484
2015 SCImago Journal Rankings: 1.583
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, YQen_HK
dc.contributor.authorLi, XTen_HK
dc.contributor.authorRabie, ABMen_HK
dc.contributor.authorFu, MKen_HK
dc.contributor.authorZhang, Den_HK
dc.date.accessioned2010-09-06T05:50:22Z-
dc.date.available2010-09-06T05:50:22Z-
dc.date.issued2006en_HK
dc.identifier.citationFrontiers In Bioscience, 2006, v. 11 n. 1 P.447-888, p. 776-781en_HK
dc.identifier.issn1093-9946en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66910-
dc.description.abstractMechanical strain applied to bone leads to bone remodeling. In the oral cavity, it is unclear how such mechanical force applied to move teeth orthodontically induces alveolar bone remodeling. It is known that osteoclasts are the only cells that are responsible for bone resorption, while the formation and activity of osteoclasts are regulated by osteoblasts. So it is believed that osteoblasts play an important role not only in bone formation but in bone remodeling as well. Therefore, the purpose of this study was to examine the effect of mechanical force on human periodontal ligament (PDL) cells and whether they express osteoblastic characters in vitro. Methods: Human PDL cells cultured in vitro were loaded with intermittently stretching force for 24 hours. The expression of alkaline phosphatase (ALP), osteocalcin (OCN) and osteoprotegerin (OPG) were detected at mRNA and protein levels at 0, 2nd, 4th, 6th, 12th, 24th hours after intermittent force loading. Results: Without any stimulation, ALP and OPG mRNA expressions were detected in human PDL cells by in-situ hybridization, but not that of OCN mRNA. ALP mRNA signal was up-regulated and that of OPG was down-regulated by mechanical force within 24 hours. OCN mRNA expression was induced by mechanical force in the late phase of the 24-hours loading cycle. The changes in secreted proteins showed similar results with those seen at the mRNA level. Conclusion: Human PDL cells express osteoblastic phenotypes under intermittent force loading and play a role in alveolar bone remodeling.en_HK
dc.languageengen_HK
dc.publisherFrontiers in Bioscience. The Journal's web site is located at http://www.frontbiosci.org/en_HK
dc.relation.ispartofFrontiers in Bioscienceen_HK
dc.subjectHuman periodontal ligament cellen_HK
dc.subjectMechanical forceen_HK
dc.subjectOsteoblasten_HK
dc.subject.meshAlkaline Phosphatase - metabolismen_HK
dc.subject.meshBone Remodelingen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshDown-Regulationen_HK
dc.subject.meshFibroblasts - metabolismen_HK
dc.subject.meshGlycoproteins - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshModels, Statisticalen_HK
dc.subject.meshOsteoblasts - cytology - metabolismen_HK
dc.subject.meshOsteocalcin - metabolismen_HK
dc.subject.meshOsteoclasts - metabolismen_HK
dc.subject.meshOsteoprotegerinen_HK
dc.subject.meshPeriodontal Ligament - cytologyen_HK
dc.subject.meshPhenotypeen_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshReceptors, Cytoplasmic and Nuclear - metabolismen_HK
dc.subject.meshReceptors, Tumor Necrosis Factor - metabolismen_HK
dc.subject.meshStress, Mechanicalen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshUp-Regulationen_HK
dc.titleHuman periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitroen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1093-9946&volume=11&spage=776&epage=781&date=2006&atitle=Human+periodontal+ligament+cells+express+osteoblastic+phenotypes+under+intermittent+force+loading+in+vitroen_HK
dc.identifier.emailYang, YQ: yangyanq@hkucc.hku.hken_HK
dc.identifier.emailRabie, ABM: rabie@hku.hken_HK
dc.identifier.authorityYang, YQ=rp00045en_HK
dc.identifier.authorityRabie, ABM=rp00029en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.2741/1835en_HK
dc.identifier.pmid16146769-
dc.identifier.scopuseid_2-s2.0-32944465522en_HK
dc.identifier.hkuros110879en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-32944465522&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue1 P.447-888en_HK
dc.identifier.spage776en_HK
dc.identifier.epage781en_HK
dc.identifier.eissn1093-4715-
dc.identifier.isiWOS:000232528000061-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYang, YQ=36623085300en_HK
dc.identifier.scopusauthoridLi, XT=7501695661en_HK
dc.identifier.scopusauthoridRabie, ABM=7007172734en_HK
dc.identifier.scopusauthoridFu, MK=7202031177en_HK
dc.identifier.scopusauthoridZhang, D=7405356257en_HK

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