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Article: Biofilm formation of Candida albicans is variably affected by saliva and dietary sugars

TitleBiofilm formation of Candida albicans is variably affected by saliva and dietary sugars
Authors
KeywordsAdhesion
ATP bioluminescence
Biofilm
Candida albicans
XTT reduction assay
Issue Date2004
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2004, v. 49 n. 10, p. 789-798 How to Cite?
AbstractThe pathogenesis of both superficial and systemic candidiasis is closely dictated by properties of the yeast biofilms. Despite extensive investigations on bacterial biofilms, the characteristics of candidal biofilms, and various factors affecting this process remain to be determined. Therefore we examined the effect of human whole saliva and dietary sugars, glucose and galactose on the adhesion and biofilm formation of Candida albicans. Biofilms of C. albicans isolate 192887g were developed on polystyrene, flat-bottomed 96-well microtiter plates and monitored using ATP bioluminescence and tetrazolium (XTT) reduction assays as well as the conventional colony forming unit (CFU) evaluation. Our data showed that both the ATP and the XTT assays strongly correlated with the CFU assay (ATP versus CFU: r = 0.994, P = 0.006; XTT versus CFU: r = 0.985, P = 0.015). Compared with a glucose - supplemented (100mM) medium, galactose containing (500mM) medium generated consistently lower levels of both candidal adhesion and biofilm formation (all P < 0.05), but a higher pace of biofilm development over time (96h). Whist the presence of an immobilised saliva coating had little effect on either the candidal adhesion or biofilm formation, the addition of saliva to the incubation medium quantitatively affected biofilm formation especially on day 3 and 4, without any significant effect on yeast adhesion. To conclude, biofilm formation of C. albicans within the oral milieu appears to be modulated to varying extents by dietary and salivary factors and, further investigations are required to elucidate these complex interactions. © 2004 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/66849
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, Yen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorSamaranayake, Yen_HK
dc.contributor.authorYip, HKen_HK
dc.date.accessioned2010-09-06T05:49:52Z-
dc.date.available2010-09-06T05:49:52Z-
dc.date.issued2004en_HK
dc.identifier.citationArchives Of Oral Biology, 2004, v. 49 n. 10, p. 789-798en_HK
dc.identifier.issn0003-9969en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66849-
dc.description.abstractThe pathogenesis of both superficial and systemic candidiasis is closely dictated by properties of the yeast biofilms. Despite extensive investigations on bacterial biofilms, the characteristics of candidal biofilms, and various factors affecting this process remain to be determined. Therefore we examined the effect of human whole saliva and dietary sugars, glucose and galactose on the adhesion and biofilm formation of Candida albicans. Biofilms of C. albicans isolate 192887g were developed on polystyrene, flat-bottomed 96-well microtiter plates and monitored using ATP bioluminescence and tetrazolium (XTT) reduction assays as well as the conventional colony forming unit (CFU) evaluation. Our data showed that both the ATP and the XTT assays strongly correlated with the CFU assay (ATP versus CFU: r = 0.994, P = 0.006; XTT versus CFU: r = 0.985, P = 0.015). Compared with a glucose - supplemented (100mM) medium, galactose containing (500mM) medium generated consistently lower levels of both candidal adhesion and biofilm formation (all P < 0.05), but a higher pace of biofilm development over time (96h). Whist the presence of an immobilised saliva coating had little effect on either the candidal adhesion or biofilm formation, the addition of saliva to the incubation medium quantitatively affected biofilm formation especially on day 3 and 4, without any significant effect on yeast adhesion. To conclude, biofilm formation of C. albicans within the oral milieu appears to be modulated to varying extents by dietary and salivary factors and, further investigations are required to elucidate these complex interactions. © 2004 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_HK
dc.relation.ispartofArchives of Oral Biologyen_HK
dc.subjectAdhesionen_HK
dc.subjectATP bioluminescenceen_HK
dc.subjectBiofilmen_HK
dc.subjectCandida albicansen_HK
dc.subjectXTT reduction assayen_HK
dc.subject.meshAdenosine Triphosphate - analysisen_HK
dc.subject.meshBiofilms - growth & developmenten_HK
dc.subject.meshCandida albicans - physiologyen_HK
dc.subject.meshCandidiasis, Oral - physiopathologyen_HK
dc.subject.meshColony Count, Microbial - methodsen_HK
dc.subject.meshCulture Mediaen_HK
dc.subject.meshDietary Carbohydrates - administration & dosageen_HK
dc.subject.meshGalactose - administration & dosage - metabolismen_HK
dc.subject.meshGlucose - administration & dosage - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMouth - microbiologyen_HK
dc.subject.meshSaliva - metabolismen_HK
dc.titleBiofilm formation of Candida albicans is variably affected by saliva and dietary sugarsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-9969&volume=49&spage=789&epage=798&date=2004&atitle=Biofilm+formation+of+Candida+albicans+is+variably+affected+by+saliva+and+dietary+sugarsen_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_HK
dc.identifier.emailSamaranayake, Y: hema@hkucc.hku.hken_HK
dc.identifier.emailYip, HK: kevin.h.k.yip@hkusua.hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authoritySamaranayake, Y=rp00025en_HK
dc.identifier.authorityYip, HK=rp00027en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.archoralbio.2004.04.011en_HK
dc.identifier.pmid15308423-
dc.identifier.scopuseid_2-s2.0-4143050182en_HK
dc.identifier.hkuros89701en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-4143050182&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume49en_HK
dc.identifier.issue10en_HK
dc.identifier.spage789en_HK
dc.identifier.epage798en_HK
dc.identifier.isiWOS:000223519700004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridJin, Y=55215762600en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridSamaranayake, Y=6602677237en_HK
dc.identifier.scopusauthoridYip, HK=25423244900en_HK

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