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Article: An immunohistochemical study on neovascularization in TMJ during mandibular advancement

TitleAn immunohistochemical study on neovascularization in TMJ during mandibular advancement
下頜前導引發顳下頜關節的新生血管反應
Authors
KeywordsBite-jumping (下頜前導)
Neovascularization (新生血管)
Immunohistochemistry (免疫組化)
Issue Date2003
PublisherShanghai Di Er Yi Ke Da Xue Kou Qiang Yi Xue Yuan (上海第二醫科大口腔醫學院). The Journal's web site is located at http://shky.chinajournal.net.cn/
Citation
Shanghai Journal Of Stomatology, 2003, v. 12 n. 2, p. 115-119 How to Cite?
上海口腔醫學, 2003, v. 12 n. 2, p. 115-119 How to Cite?
AbstractOBJECTIVE: Neovascularization is proved to be closely correlated with ossification. This study was designed to determine the biochemical path through which the new bone formation is enhanced in TMJ during mandibular protrusion. METHODS: SD Rats were fitted with bite-jumping appliances to allow for an adequate advancement of the mandible. The animals were sacrificed 3, 7, 14, 21 and 30 days, respectively, after placement of the appliances. Slides with TMJ tissue were prepared for biochemical procedure. Immunohistochemical approaches were adopted to examine neovascularization in TMJ by immuno-localizing the newly formed endothelial cells. Computer-assisted image analysis system was applied to quantify the positive immunoreaction. RESULTS: Neovascularization in connective tissue close to the condyle was 200% higher in the experimental groups than that in the control, and, within the bony tissue of the condyle, neovascularization in experimental animals was found to be 103% higher than that of the control. CONCLUSION: Increased neovascularization triggered by mandibular protrusion might intensify new bone formation in condyle and glenoid fossa and finally lead to a growth adaptation of TMJ to bite-jumping therapy. 目的依據顳下頜關節(TMJ)髁突軟骨內成骨及關節凹膜內成骨的生長模式,觀察TMJ在下頜前導位時的新生血管反應,在分子生物學水平探究新生微細血管增殖在TMJ骨組織改建中的作用。方法35天雌性Sprague-Dawly大鼠隨機分成5個實驗組和5個對照組。實驗組動物戴用咬合前導功能性矯正器(BJA),造成持續的下頜前導。實驗組和對照組動物分別在第3、7、14、21、30天時被處死。取下TMJ組織,石蠟包埋,切片,用于免疫組化分析。使用單克隆抗體EN7/44對新生血管內皮細胞進行免疫染色,評價新生血管形成。采用LeicaQwin圖像輔助分析系統,對新生血管進行定量分析。結果(1)連接髁突和關節窩后面的結締纖維組織中,標志新生血管的免疫染色實驗組比對照組平均高200%(P<0.01);(2)在髁突和關節窩骨組織的新生血管免疫染色中,實驗組比對照組分別高103%和163%(P<0.01)。結論下頜前導后TMJ軟硬組織內新生血管形成增多,這可能是導致TMJ新骨增加的重要機制之一。
Persistent Identifierhttp://hdl.handle.net/10722/66801
ISSN
2015 SCImago Journal Rankings: 0.120

 

DC FieldValueLanguage
dc.contributor.authorShen, Gen_HK
dc.contributor.authorRabie, ABen_HK
dc.contributor.authorHägg, Uen_HK
dc.contributor.authorChen, RJen_HK
dc.date.accessioned2010-09-06T05:49:28Z-
dc.date.available2010-09-06T05:49:28Z-
dc.date.issued2003en_HK
dc.identifier.citationShanghai Journal Of Stomatology, 2003, v. 12 n. 2, p. 115-119en_HK
dc.identifier.citation上海口腔醫學, 2003, v. 12 n. 2, p. 115-119-
dc.identifier.issn1006-7248en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66801-
dc.description.abstractOBJECTIVE: Neovascularization is proved to be closely correlated with ossification. This study was designed to determine the biochemical path through which the new bone formation is enhanced in TMJ during mandibular protrusion. METHODS: SD Rats were fitted with bite-jumping appliances to allow for an adequate advancement of the mandible. The animals were sacrificed 3, 7, 14, 21 and 30 days, respectively, after placement of the appliances. Slides with TMJ tissue were prepared for biochemical procedure. Immunohistochemical approaches were adopted to examine neovascularization in TMJ by immuno-localizing the newly formed endothelial cells. Computer-assisted image analysis system was applied to quantify the positive immunoreaction. RESULTS: Neovascularization in connective tissue close to the condyle was 200% higher in the experimental groups than that in the control, and, within the bony tissue of the condyle, neovascularization in experimental animals was found to be 103% higher than that of the control. CONCLUSION: Increased neovascularization triggered by mandibular protrusion might intensify new bone formation in condyle and glenoid fossa and finally lead to a growth adaptation of TMJ to bite-jumping therapy. 目的依據顳下頜關節(TMJ)髁突軟骨內成骨及關節凹膜內成骨的生長模式,觀察TMJ在下頜前導位時的新生血管反應,在分子生物學水平探究新生微細血管增殖在TMJ骨組織改建中的作用。方法35天雌性Sprague-Dawly大鼠隨機分成5個實驗組和5個對照組。實驗組動物戴用咬合前導功能性矯正器(BJA),造成持續的下頜前導。實驗組和對照組動物分別在第3、7、14、21、30天時被處死。取下TMJ組織,石蠟包埋,切片,用于免疫組化分析。使用單克隆抗體EN7/44對新生血管內皮細胞進行免疫染色,評價新生血管形成。采用LeicaQwin圖像輔助分析系統,對新生血管進行定量分析。結果(1)連接髁突和關節窩后面的結締纖維組織中,標志新生血管的免疫染色實驗組比對照組平均高200%(P<0.01);(2)在髁突和關節窩骨組織的新生血管免疫染色中,實驗組比對照組分別高103%和163%(P<0.01)。結論下頜前導后TMJ軟硬組織內新生血管形成增多,這可能是導致TMJ新骨增加的重要機制之一。en_HK
dc.languagechien_HK
dc.publisherShanghai Di Er Yi Ke Da Xue Kou Qiang Yi Xue Yuan (上海第二醫科大口腔醫學院). The Journal's web site is located at http://shky.chinajournal.net.cn/en_HK
dc.relation.ispartofShanghai journal of stomatologyen_HK
dc.relation.ispartof上海口腔醫學-
dc.subjectBite-jumping (下頜前導)-
dc.subjectNeovascularization (新生血管)-
dc.subjectImmunohistochemistry (免疫組化)-
dc.subject.meshAnimalsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshMandibular Advancementen_HK
dc.subject.meshNeovascularization, Physiologicen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshTemporomandibular Joint - blood supplyen_HK
dc.titleAn immunohistochemical study on neovascularization in TMJ during mandibular advancementen_HK
dc.title下頜前導引發顳下頜關節的新生血管反應-
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1006-7248&volume=12&issue=2&spage=115&epage=119&date=2003&atitle=An+immunohistochemical+study+on+neovascularization+in+TMJ+during+mandibular+advancement.en_HK
dc.identifier.emailRabie, AB: rabie@hku.hken_HK
dc.identifier.emailHägg, U: euohagg@hkusua.hku.hken_HK
dc.identifier.authorityRabie, AB=rp00029en_HK
dc.identifier.authorityHägg, U=rp00020en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid14661516-
dc.identifier.scopuseid_2-s2.0-0642307102en_HK
dc.identifier.hkuros76687en_HK
dc.identifier.volume12en_HK
dc.identifier.issue2en_HK
dc.identifier.spage115en_HK
dc.identifier.epage119en_HK
dc.publisher.placeChina (中國)en_HK
dc.identifier.scopusauthoridShen, G=7401966958en_HK
dc.identifier.scopusauthoridRabie, AB=7007172734en_HK
dc.identifier.scopusauthoridHägg, U=7006790279en_HK
dc.identifier.scopusauthoridChen, RJ=37087050700en_HK
dc.customcontrol.immutablecsl 151218-

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