Article: Exploring the oral bacterial flora: Current status and future directions
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- Publisher Website: 10.1111/j.1601-0825.2009.01607.x
- Scopus: eid_2-s2.0-77249086715
- PMID: 19627515
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| Title | Exploring the oral bacterial flora: Current status and future directions | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Parahitiyawa, NB3 Scully, C1 Leung, WK3 Yam, WC2 Jin, LJ3 Samaranayake, LP3 | ||||||
| Keywords | Immunology and microbiology | ||||||
| Issue Date | 2010 | ||||||
| Publisher | Wiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1 | ||||||
| Citation | Oral Diseases, 2010, v. 16 n. 2, p. 136-145 [How to Cite?] DOI: http://dx.doi.org/10.1111/j.1601-0825.2009.01607.x | ||||||
| Abstract | Objective: The oral cavity forms an indispensable part of the human microbiome, for its unique and diverse microflora distributed within various niches. While majority of these organisms exhibit commensalism, shifts in bacterial community dynamics cause pathological changes within oral cavity and distant sites. The aim of this review was to appraise the current and emerging methods of detecting bacteria of the oral cavity paying particular attention to the cultivation independent methods. Design: Literature pertaining to cultivation based and cultivation independent methods of oral bacterial identification was reviewed. Methods: The specific advantages and disadvantages of cultivation based, microscopic, immunological and metagenomic identification methods were appraised. Results: Because of their fastidious and exacting growth requirements, cultivation based studies grossly underestimate the extent of bacterial diversity in these polymicrobial infections. Culture independent methods deemed more sensitive in identifying difficult to culture and novel bacterial species. Conclusion: Apart from characterizing potentially novel bacterial species, the nucleic acid sequence data analyzed using various bioinformatics protocols have revealed that there are in excess of 700 bacterial species inhabiting the mouth. Moreover, the latest pyrosequencing based methods have further broadened the extent of bacterial diversity in oral niches. © 2009 John Wiley & Sons A/S. | ||||||
| ISSN | 1354-523X 2011 Impact Factor: 2.495 2011 SCImago Journal Rankings: 0.168 | ||||||
| DOI | http://dx.doi.org/10.1111/j.1601-0825.2009.01607.x | ||||||
| ISI Accession Number ID | WOS:000274713400003
Funding Information: The Authors wish to acknowledge the financial support to this work from the Hong Kong Research Grants Council (Grant RGC HKU 7624/06M to L.P.S. and Grant HKU7518/05M to L.J.J.) and the Strategic Research Theme on Infection and Immunity of The University of Hong Kong. | ||||||
| References | References in Scopus |
| dc.contributor.author | Parahitiyawa, NB | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Scully, C | ||||||
| dc.contributor.author | Leung, WK | ||||||
| dc.contributor.author | Yam, WC | ||||||
| dc.contributor.author | Jin, LJ | ||||||
| dc.contributor.author | Samaranayake, LP | ||||||
| dc.date.accessioned | 2010-09-06T05:49:22Z | ||||||
| dc.date.available | 2010-09-06T05:49:22Z | ||||||
| dc.date.issued | 2010 | ||||||
| dc.description.abstract | Objective: The oral cavity forms an indispensable part of the human microbiome, for its unique and diverse microflora distributed within various niches. While majority of these organisms exhibit commensalism, shifts in bacterial community dynamics cause pathological changes within oral cavity and distant sites. The aim of this review was to appraise the current and emerging methods of detecting bacteria of the oral cavity paying particular attention to the cultivation independent methods. Design: Literature pertaining to cultivation based and cultivation independent methods of oral bacterial identification was reviewed. Methods: The specific advantages and disadvantages of cultivation based, microscopic, immunological and metagenomic identification methods were appraised. Results: Because of their fastidious and exacting growth requirements, cultivation based studies grossly underestimate the extent of bacterial diversity in these polymicrobial infections. Culture independent methods deemed more sensitive in identifying difficult to culture and novel bacterial species. Conclusion: Apart from characterizing potentially novel bacterial species, the nucleic acid sequence data analyzed using various bioinformatics protocols have revealed that there are in excess of 700 bacterial species inhabiting the mouth. Moreover, the latest pyrosequencing based methods have further broadened the extent of bacterial diversity in oral niches. © 2009 John Wiley & Sons A/S. | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Oral Diseases, 2010, v. 16 n. 2, p. 136-145 [How to Cite?] DOI: http://dx.doi.org/10.1111/j.1601-0825.2009.01607.x | ||||||
| dc.identifier.citeulike | 6841665 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1111/j.1601-0825.2009.01607.x | ||||||
| dc.identifier.epage | 145 | ||||||
| dc.identifier.hkuros | 169117 | ||||||
| dc.identifier.isi | WOS:000274713400003
Funding Information: The Authors wish to acknowledge the financial support to this work from the Hong Kong Research Grants Council (Grant RGC HKU 7624/06M to L.P.S. and Grant HKU7518/05M to L.J.J.) and the Strategic Research Theme on Infection and Immunity of The University of Hong Kong. | ||||||
| dc.identifier.issn | 1354-523X 2011 Impact Factor: 2.495 2011 SCImago Journal Rankings: 0.168 | ||||||
| dc.identifier.issue | 2 | ||||||
| dc.identifier.openurl | | ||||||
| dc.identifier.pmid | 19627515 | ||||||
| dc.identifier.scopus | eid_2-s2.0-77249086715 | ||||||
| dc.identifier.spage | 136 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/66790 | ||||||
| dc.identifier.volume | 16 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Wiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1 | ||||||
| dc.publisher.place | United States | ||||||
| dc.relation.ispartof | Oral Diseases | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Bacteria - classification - growth & development | ||||||
| dc.subject.mesh | Bacteriological Techniques | ||||||
| dc.subject.mesh | Biodiversity | ||||||
| dc.subject.mesh | Humans | ||||||
| dc.subject.mesh | Metagenome | ||||||
| dc.subject.mesh | Metagenomics | ||||||
| dc.subject.mesh | Mouth - microbiology | ||||||
| dc.subject.mesh | Mouth Diseases - microbiology | ||||||
| dc.subject.mesh | Tooth Diseases - microbiology | ||||||
| dc.subject | Immunology and microbiology | ||||||
| dc.title | Exploring the oral bacterial flora: Current status and future directions | ||||||
| dc.type | Article |
Author Affiliations
- UCL
- The University of Hong Kong Li Ka Shing Faculty of Medicine
- The University of Hong Kong