File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Exploring the oral bacterial flora: Current status and future directions

TitleExploring the oral bacterial flora: Current status and future directions
Authors
KeywordsImmunology and microbiology
Issue Date2010
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1
Citation
Oral Diseases, 2010, v. 16 n. 2, p. 136-145 How to Cite?
AbstractObjective: The oral cavity forms an indispensable part of the human microbiome, for its unique and diverse microflora distributed within various niches. While majority of these organisms exhibit commensalism, shifts in bacterial community dynamics cause pathological changes within oral cavity and distant sites. The aim of this review was to appraise the current and emerging methods of detecting bacteria of the oral cavity paying particular attention to the cultivation independent methods. Design: Literature pertaining to cultivation based and cultivation independent methods of oral bacterial identification was reviewed. Methods: The specific advantages and disadvantages of cultivation based, microscopic, immunological and metagenomic identification methods were appraised. Results: Because of their fastidious and exacting growth requirements, cultivation based studies grossly underestimate the extent of bacterial diversity in these polymicrobial infections. Culture independent methods deemed more sensitive in identifying difficult to culture and novel bacterial species. Conclusion: Apart from characterizing potentially novel bacterial species, the nucleic acid sequence data analyzed using various bioinformatics protocols have revealed that there are in excess of 700 bacterial species inhabiting the mouth. Moreover, the latest pyrosequencing based methods have further broadened the extent of bacterial diversity in oral niches. © 2009 John Wiley & Sons A/S.
Persistent Identifierhttp://hdl.handle.net/10722/66790
ISSN
2014 Impact Factor: 2.427
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilRGC HKU 7624/06M
HKU7518/05M
Strategic Research Theme on Infection and Immunity of The University of Hong Kong
Funding Information:

The Authors wish to acknowledge the financial support to this work from the Hong Kong Research Grants Council (Grant RGC HKU 7624/06M to L.P.S. and Grant HKU7518/05M to L.J.J.) and the Strategic Research Theme on Infection and Immunity of The University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorParahitiyawa, NBen_HK
dc.contributor.authorScully, Cen_HK
dc.contributor.authorLeung, WKen_HK
dc.contributor.authorYam, WCen_HK
dc.contributor.authorJin, LJen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:49:22Z-
dc.date.available2010-09-06T05:49:22Z-
dc.date.issued2010en_HK
dc.identifier.citationOral Diseases, 2010, v. 16 n. 2, p. 136-145en_HK
dc.identifier.issn1354-523Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/66790-
dc.description.abstractObjective: The oral cavity forms an indispensable part of the human microbiome, for its unique and diverse microflora distributed within various niches. While majority of these organisms exhibit commensalism, shifts in bacterial community dynamics cause pathological changes within oral cavity and distant sites. The aim of this review was to appraise the current and emerging methods of detecting bacteria of the oral cavity paying particular attention to the cultivation independent methods. Design: Literature pertaining to cultivation based and cultivation independent methods of oral bacterial identification was reviewed. Methods: The specific advantages and disadvantages of cultivation based, microscopic, immunological and metagenomic identification methods were appraised. Results: Because of their fastidious and exacting growth requirements, cultivation based studies grossly underestimate the extent of bacterial diversity in these polymicrobial infections. Culture independent methods deemed more sensitive in identifying difficult to culture and novel bacterial species. Conclusion: Apart from characterizing potentially novel bacterial species, the nucleic acid sequence data analyzed using various bioinformatics protocols have revealed that there are in excess of 700 bacterial species inhabiting the mouth. Moreover, the latest pyrosequencing based methods have further broadened the extent of bacterial diversity in oral niches. © 2009 John Wiley & Sons A/S.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1en_HK
dc.relation.ispartofOral Diseasesen_HK
dc.subjectImmunology and microbiologyen_HK
dc.subject.meshBacteria - classification - growth & developmenten_HK
dc.subject.meshBacteriological Techniquesen_HK
dc.subject.meshBiodiversityen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMetagenomeen_HK
dc.subject.meshMetagenomicsen_HK
dc.subject.meshMouth - microbiologyen_HK
dc.subject.meshMouth Diseases - microbiologyen_HK
dc.subject.meshTooth Diseases - microbiologyen_HK
dc.titleExploring the oral bacterial flora: Current status and future directionsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1354-523X&volume=16&spage=136&epage=145&date=2010&atitle=Exploring+the+oral+bacterial+flora:+current+status+and+future+directionsen_HK
dc.identifier.emailLeung, WK:ewkleung@hkucc.hku.hken_HK
dc.identifier.emailYam, WC:wcyam@hkucc.hku.hken_HK
dc.identifier.emailJin, LJ:ljjin@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authorityLeung, WK=rp00019en_HK
dc.identifier.authorityYam, WC=rp00313en_HK
dc.identifier.authorityJin, LJ=rp00028en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1601-0825.2009.01607.xen_HK
dc.identifier.pmid19627515en_HK
dc.identifier.scopuseid_2-s2.0-77249086715en_HK
dc.identifier.hkuros169117en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77249086715&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue2en_HK
dc.identifier.spage136en_HK
dc.identifier.epage145en_HK
dc.identifier.isiWOS:000274713400003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridParahitiyawa, NB=13408948800en_HK
dc.identifier.scopusauthoridScully, C=35393576100en_HK
dc.identifier.scopusauthoridLeung, WK=25224691800en_HK
dc.identifier.scopusauthoridYam, WC=7004281720en_HK
dc.identifier.scopusauthoridJin, LJ=7403328850en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.citeulike6841665-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats