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Article: The influence of incubation conditions on the adherence of oral Enterobacteriaceae to HeLa cells

TitleThe influence of incubation conditions on the adherence of oral Enterobacteriaceae to HeLa cells
Authors
Sedgley, CMSamaranayake, LPDarvell, BW
KeywordsAdherence
Enterobacteriaceae
HeLa cells
Incubation conditions
Issue Date1996
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMIS
Citation
Apmis, 1996, v. 104 n. 7-8, p. 583-590 How to Cite?
DOI: http://dx.doi.org/10.1111/j.1699-0463.1996.tb04915.x
AbstractThe adherence of 12 oral isolates and 4 type strains of Enterobacteriaceae (equally representing Enterobacter cloacae, Klebsiella pneumoniae, Escherichia coli and Citrobacter freundii) to HeLa cell monolayers following five different incubation conditions (sucrose, D-mannose, serum, MEM and Candida albicans GDH 1957) was investigated. Incubation with sucrose and D-mannose resulted in the greatest and least adherence, respectively The presence of preadherent C. albicans GDH 1957 on the HeLa cells tended to enhance the adherence of certain strains of E. cloacae and C. freundii, but had no overall impact on Enterobacteriaceae adherence. While heterogeneity of behaviour existed between strains within species, E. cloacae was the most, and K. pneumoniae the least, adherent species irrespective of incubation conditions. Haemagglutination assays indicated the presence of mannose-resistant type 1 fimbriae associated with all Enterobacteriaceae. In clinical terms, the variations in adherence properties observed in vitro may contribute to an understanding of the different prevalence rates of oral Enterobacteriaceae reported in the literature.
Persistent Identifierhttp://hdl.handle.net/10722/66734
ISSN
0903-4641
2021 Impact Factor: 3.428
2020 SCImago Journal Rankings: 0.909
ISI Accession Number ID
WOS:A1996VQ87100014
References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorSedgley, CMen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorDarvell, BWen_HK
dc.date.accessioned2010-09-06T05:48:53Z-
dc.date.available2010-09-06T05:48:53Z-
dc.date.issued1996en_HK
dc.identifier.citationApmis, 1996, v. 104 n. 7-8, p. 583-590en_HK
dc.identifier.issn0903-4641en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66734-
dc.description.abstractThe adherence of 12 oral isolates and 4 type strains of Enterobacteriaceae (equally representing Enterobacter cloacae, Klebsiella pneumoniae, Escherichia coli and Citrobacter freundii) to HeLa cell monolayers following five different incubation conditions (sucrose, D-mannose, serum, MEM and Candida albicans GDH 1957) was investigated. Incubation with sucrose and D-mannose resulted in the greatest and least adherence, respectively The presence of preadherent C. albicans GDH 1957 on the HeLa cells tended to enhance the adherence of certain strains of E. cloacae and C. freundii, but had no overall impact on Enterobacteriaceae adherence. While heterogeneity of behaviour existed between strains within species, E. cloacae was the most, and K. pneumoniae the least, adherent species irrespective of incubation conditions. Haemagglutination assays indicated the presence of mannose-resistant type 1 fimbriae associated with all Enterobacteriaceae. In clinical terms, the variations in adherence properties observed in vitro may contribute to an understanding of the different prevalence rates of oral Enterobacteriaceae reported in the literature.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMISen_HK
dc.relation.ispartofAPMISen_HK
dc.subjectAdherenceen_HK
dc.subjectEnterobacteriaceaeen_HK
dc.subjectHeLa cellsen_HK
dc.subjectIncubation conditionsen_HK
dc.subject.meshBacterial Adhesionen_HK
dc.subject.meshCandida albicans - pathogenicityen_HK
dc.subject.meshCarbohydratesen_HK
dc.subject.meshCulture Mediaen_HK
dc.subject.meshEnterobacteriaceae - pathogenicityen_HK
dc.subject.meshFimbriae, Bacterial - physiologyen_HK
dc.subject.meshHeLa Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMouth Diseases - microbiologyen_HK
dc.titleThe influence of incubation conditions on the adherence of oral Enterobacteriaceae to HeLa cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0903-4641&volume=104&spage=583&epage=590&date=1996&atitle=The+influence+of+incubation+conditions+on+the+adherence+of+oral+enterobacteriaceae+to+HeLa+cellsen_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_HK
dc.identifier.emailDarvell, BW: b.w.darvell@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authorityDarvell, BW=rp00007en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1699-0463.1996.tb04915.x-
dc.identifier.pmid8920813-
dc.identifier.scopuseid_2-s2.0-0029989360en_HK
dc.identifier.hkuros23393en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029989360&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume104en_HK
dc.identifier.issue7-8en_HK
dc.identifier.spage583en_HK
dc.identifier.epage590en_HK
dc.identifier.isiWOS:A1996VQ87100014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSedgley, CM=6701569363en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridDarvell, BW=7005953926en_HK
dc.identifier.issnl0903-4641-

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