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Article: The use of new probes and stains for improved assessment of cell viability and extracellular polymeric substances in Candida albicans biofilms

TitleThe use of new probes and stains for improved assessment of cell viability and extracellular polymeric substances in Candida albicans biofilms
Authors
KeywordsBiofilm
Candida albicans
Confocal scanning laser microscope
Differentiation
Stain
Issue Date2005
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0301-486X
Citation
Mycopathologia, 2005, v. 159 n. 3, p. 353-360 How to Cite?
AbstractPhenotypic and genotypic cell differentiation is considered an important feature that confers enhanced antifungal resistance in candidal biofilms. Particular emphasis has been placed in this context on the viability of biofilm subpopulations, and their heterogeneity with regard to the production of extracellular polymeric substances (EPS). We therefore assessed the utility of two different labeled lectins Erythrina cristagalli (ECA) and Canavalia ensiformis (ConA), for EPS visualization. To evaluate the viability of candidal biofilms, we further studied combination stains, SYTO9 and propidium iodide (PI). The latter combination has been successfully used to assess bacterial, but not fungal, viability although PI alone has been previously used to stain nuclei in fungal cells. Candida albicans biofilms were developed in a rotating disc biofilm reactor and observed in situ using confocal scanning laser microscopy (CSLM). Our data indicate that SYTO9 and PI are reliable vital stains that may be used to investigate C. albicans biofilms. When used together with ConA, the lectin ECA optimized EPS visualization and revealed differential production of this material in mature candidal biofilms. The foregoing probes and stains and the methodology described should help better characterize C. albicans biofilms in terms of cell their viability, and EPS production. © Springer 2005.
Persistent Identifierhttp://hdl.handle.net/10722/66718
ISSN
2015 Impact Factor: 1.671
2015 SCImago Journal Rankings: 0.720
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, Yen_HK
dc.contributor.authorZhang, Ten_HK
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorFang, HHPen_HK
dc.contributor.authorYip, HKen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:48:45Z-
dc.date.available2010-09-06T05:48:45Z-
dc.date.issued2005en_HK
dc.identifier.citationMycopathologia, 2005, v. 159 n. 3, p. 353-360en_HK
dc.identifier.issn0301-486Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/66718-
dc.description.abstractPhenotypic and genotypic cell differentiation is considered an important feature that confers enhanced antifungal resistance in candidal biofilms. Particular emphasis has been placed in this context on the viability of biofilm subpopulations, and their heterogeneity with regard to the production of extracellular polymeric substances (EPS). We therefore assessed the utility of two different labeled lectins Erythrina cristagalli (ECA) and Canavalia ensiformis (ConA), for EPS visualization. To evaluate the viability of candidal biofilms, we further studied combination stains, SYTO9 and propidium iodide (PI). The latter combination has been successfully used to assess bacterial, but not fungal, viability although PI alone has been previously used to stain nuclei in fungal cells. Candida albicans biofilms were developed in a rotating disc biofilm reactor and observed in situ using confocal scanning laser microscopy (CSLM). Our data indicate that SYTO9 and PI are reliable vital stains that may be used to investigate C. albicans biofilms. When used together with ConA, the lectin ECA optimized EPS visualization and revealed differential production of this material in mature candidal biofilms. The foregoing probes and stains and the methodology described should help better characterize C. albicans biofilms in terms of cell their viability, and EPS production. © Springer 2005.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0301-486Xen_HK
dc.relation.ispartofMycopathologiaen_HK
dc.subjectBiofilmen_HK
dc.subjectCandida albicansen_HK
dc.subjectConfocal scanning laser microscopeen_HK
dc.subjectDifferentiationen_HK
dc.subjectStainen_HK
dc.subject.meshBiofilms - growth & developmenten_HK
dc.subject.meshCanavaliaen_HK
dc.subject.meshCandida albicans - growth & development - physiology - ultrastructureen_HK
dc.subject.meshErythrinaen_HK
dc.subject.meshIndicators and Reagentsen_HK
dc.subject.meshLectinsen_HK
dc.subject.meshMicroscopy, Confocal - methodsen_HK
dc.subject.meshOrganic Chemicalsen_HK
dc.subject.meshPlant Lectinsen_HK
dc.subject.meshPolymers - analysisen_HK
dc.subject.meshPropidiumen_HK
dc.titleThe use of new probes and stains for improved assessment of cell viability and extracellular polymeric substances in Candida albicans biofilmsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0301-486X&volume=159&spage=353&epage=360&date=2005&atitle=The+use+of+new+probes+and+stains+for+improved+assessment+of+cell+viability+and+extracellular+polymeric+substances+in+Candida+albicans+biofilmsen_HK
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, YH: hema@hkucc.hku.hken_HK
dc.identifier.emailFang, HHP: hrechef@hkucc.hku.hken_HK
dc.identifier.emailYip, HK: kevin.h.k.yip@hkusua.hku.hken_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_HK
dc.identifier.authorityZhang, T=rp00211en_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.identifier.authorityYip, HK=rp00027en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s11046-004-6987-7en_HK
dc.identifier.pmid15883718-
dc.identifier.scopuseid_2-s2.0-19944421098en_HK
dc.identifier.hkuros98106en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-19944421098&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume159en_HK
dc.identifier.issue3en_HK
dc.identifier.spage353en_HK
dc.identifier.epage360en_HK
dc.identifier.isiWOS:000229004100005-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridJin, Y=55215762600en_HK
dc.identifier.scopusauthoridZhang, T=24470677400en_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK
dc.identifier.scopusauthoridYip, HK=25423244900en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.citeulike199804-

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