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Article: Granulocyte elastase activity in static and flow gingival crevicular fluid

TitleGranulocyte elastase activity in static and flow gingival crevicular fluid
Authors
Issue Date2003
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1
Citation
Journal Of Periodontal Research, 2003, v. 38 n. 3, p. 303-310 How to Cite?
AbstractObjectives: This study aimed to evaluate the volume of gingival crevicular fluid (GCF) and granulocyte elastase activity in static GCF (sGCF) and flow GCF (fGCF) from subjects with various periodontal conditions. Methods: Eleven periodontally healthy, 10 gingivitis and 12 periodontitis subjects were recruited and the sites investigated consisted of healthy sites from healthy subjects (HH); healthy (HG) and gingivitis sites (GG) from gingivitis subjects; and healthy (HP), gingivitis (GP) and periodontitis sites (PP) from periodontitis subjects. fGCF samples were collected either 1 min or 5 min following sGCF collection by paper strip technique. GCF volume was determined by Periotron® 6000 and granulocyte elastase activity was assayed with a specific substrate [L-pyroglutamyl-L-prolyl-L-valine-p-nitroanilide(pGluProVal- pNA)]. Results: At baseline, no significant differences existed in clinical and GCF parameters between the two matched sites for subsequent collection of fGCF samples either 1 min or 5 min after sGCF sampling in all subjects. The flow exudate in HG and HP sites quickly replenished to sGCF levels, while a delayed replenishment was found in HH sites, despite the similar sGCF volumes of these sites. The GCF volume and elastase levels in the fGCF at 1 min were higher in GP sites than in GG sites (P < 0.05). Overall, depletion of elastase levels in the fGCF at 1 min was observed in all subjects, whereas elastase levels in the fGCF at 5 min had replenished to sGCF levels in HP, GP, PP sites and GG sites, but had remained at a lower level in HH and HG sites. An overall positive correlation was found between sGCF and fGCF for GCF volume and elastase activity (P < 0.001); however, this correlation varied with GCF parameters and with site conditions of the subjects concerned. Conclusions: This study shows that patterns of dynamic changes in GCF flow and elastase activity varied under different periodontal conditions. Assessment of both sGCF and fGCF may allow better insight into the dynamic change of the target components in GCF.
Persistent Identifierhttp://hdl.handle.net/10722/66716
ISSN
2015 Impact Factor: 2.474
2015 SCImago Journal Rankings: 0.932
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, Len_HK
dc.contributor.authorYu, Cen_HK
dc.contributor.authorCorbet, EFen_HK
dc.date.accessioned2010-09-06T05:48:43Z-
dc.date.available2010-09-06T05:48:43Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Periodontal Research, 2003, v. 38 n. 3, p. 303-310en_HK
dc.identifier.issn0022-3484en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66716-
dc.description.abstractObjectives: This study aimed to evaluate the volume of gingival crevicular fluid (GCF) and granulocyte elastase activity in static GCF (sGCF) and flow GCF (fGCF) from subjects with various periodontal conditions. Methods: Eleven periodontally healthy, 10 gingivitis and 12 periodontitis subjects were recruited and the sites investigated consisted of healthy sites from healthy subjects (HH); healthy (HG) and gingivitis sites (GG) from gingivitis subjects; and healthy (HP), gingivitis (GP) and periodontitis sites (PP) from periodontitis subjects. fGCF samples were collected either 1 min or 5 min following sGCF collection by paper strip technique. GCF volume was determined by Periotron® 6000 and granulocyte elastase activity was assayed with a specific substrate [L-pyroglutamyl-L-prolyl-L-valine-p-nitroanilide(pGluProVal- pNA)]. Results: At baseline, no significant differences existed in clinical and GCF parameters between the two matched sites for subsequent collection of fGCF samples either 1 min or 5 min after sGCF sampling in all subjects. The flow exudate in HG and HP sites quickly replenished to sGCF levels, while a delayed replenishment was found in HH sites, despite the similar sGCF volumes of these sites. The GCF volume and elastase levels in the fGCF at 1 min were higher in GP sites than in GG sites (P < 0.05). Overall, depletion of elastase levels in the fGCF at 1 min was observed in all subjects, whereas elastase levels in the fGCF at 5 min had replenished to sGCF levels in HP, GP, PP sites and GG sites, but had remained at a lower level in HH and HG sites. An overall positive correlation was found between sGCF and fGCF for GCF volume and elastase activity (P < 0.001); however, this correlation varied with GCF parameters and with site conditions of the subjects concerned. Conclusions: This study shows that patterns of dynamic changes in GCF flow and elastase activity varied under different periodontal conditions. Assessment of both sGCF and fGCF may allow better insight into the dynamic change of the target components in GCF.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0022-3484&site=1en_HK
dc.relation.ispartofJournal of Periodontal Researchen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAlveolar Bone Loss - enzymologyen_HK
dc.subject.meshAnalysis of Varianceen_HK
dc.subject.meshCase-Control Studiesen_HK
dc.subject.meshChronic Diseaseen_HK
dc.subject.meshDental Plaque Indexen_HK
dc.subject.meshGingival Crevicular Fluid - enzymology - secretionen_HK
dc.subject.meshGingivitis - enzymologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLeukocyte Elastase - analysisen_HK
dc.subject.meshLinear Modelsen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPeriodontal Attachment Loss - enzymologyen_HK
dc.subject.meshPeriodontal Indexen_HK
dc.subject.meshPeriodontal Pocket - enzymologyen_HK
dc.subject.meshPeriodontitis - enzymologyen_HK
dc.subject.meshPeriodontium - enzymologyen_HK
dc.subject.meshSecretory Rateen_HK
dc.subject.meshTime Factorsen_HK
dc.titleGranulocyte elastase activity in static and flow gingival crevicular fluiden_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-3484&volume=38&spage=303&epage=310&date=2003&atitle=Granulocyte+elastase+activity+in+static+and+flow+gingival+crevicular+fluiden_HK
dc.identifier.emailJin, L:ljjin@hkucc.hku.hken_HK
dc.identifier.emailCorbet, EF:efcorbet@hku.hken_HK
dc.identifier.authorityJin, L=rp00028en_HK
dc.identifier.authorityCorbet, EF=rp00005en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid12753369-
dc.identifier.scopuseid_2-s2.0-0042760456en_HK
dc.identifier.hkuros80314en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0042760456&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume38en_HK
dc.identifier.issue3en_HK
dc.identifier.spage303en_HK
dc.identifier.epage310en_HK
dc.identifier.isiWOS:000182984800012-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridJin, L=7403328850en_HK
dc.identifier.scopusauthoridYu, C=7404977564en_HK
dc.identifier.scopusauthoridCorbet, EF=35609873200en_HK

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