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Article: Granulocyte elastase activity and PGE2 levels in gingival crevicular fluid in relation to the presence of subgingival periodontopathogens in subjects with untreated adult periodontitis

TitleGranulocyte elastase activity and PGE2 levels in gingival crevicular fluid in relation to the presence of subgingival periodontopathogens in subjects with untreated adult periodontitis
Authors
Issue Date1999
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
Citation
Journal Of Clinical Periodontology, 1999, v. 26 n. 8, p. 531-540 How to Cite?
AbstractThis study aimed to determine the association between the levels of granulocyte elastase and prostaglandin E2 (PGE2) in GCF, and the concomitant presence of periodontopathogens in untreated adult periodontitis (AP). GCF and subgingival plaque were sampled by paper strips and paper points respectively, from various periodontal sites in 16 AP subjects. Granulocyte elastase activity in GCF was analyzed with a low molecular weight substrate specific for granulocyte elastase, pGluProVal-pNA, and the maximal rate of elastase activity (MR-EA, mAbs/min/site) was calculated. PGE2 levels in GCF were determined by radio-immunoassay. 5 species-specific DNA probes were used to detect the presence of A. actinomycetemcomitans (A.a., ATCC 43718), B. forsythus (B.f., ATCC 43037), P. gingivalis (P.g., ATCC 33277), P. intermedia (P.i., ATCC 33563), and T. denticola (T.d., ATCC 35405), with a sensitivity of 103 cells/paper point. No A.a. was detectable from all sites sampled. The predominant combination of species detected was B.f., P.g., P.i. & T.d. and it was significantly higher at periodontitis sites (68%) than at healthy (7%) or gingivitis sites (29%) (p<0.05). Overall, MR-EA values were strongly correlated with PGE2 levels (r=0.655, p<0.001), especially at these periodontitis sites co-infected by B.f., P.g., P.i. & T.d. (r=0.722, p<0.001). The periodontitis sites co-infected by the 4 species were observable from 15 subjects. These sites were sub-grouped into 8 subjects with a high MR-EA and 7 subjects with a low MR-EA. The PGE2 levels in the high MR-EA group were significantly higher than in the low MR-EA group (p<0.05). No significant differences in clinical or bacterial data were found between the two groups. While within the high MR-EA group, similar results were found between the paired periodontitis sites in each subject with highest and lowest MR-EA values. This study shows that the local host response to bacterial challenge in untreated periodontal pockets is diverse in terms of the intensity of inflammatory response measured by granulocyte elastase and PGE2 levels in GCF. A more thorough evaluation of the risk for active periodontal disease may involve the combined approaches to the test of the dynamic bacteria-host relations. © Munksgaard, 1999.
Persistent Identifierhttp://hdl.handle.net/10722/66676
ISSN
2015 Impact Factor: 3.915
2015 SCImago Journal Rankings: 1.848
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, LJen_HK
dc.contributor.authorSöder, PÖen_HK
dc.contributor.authorLeung, WKen_HK
dc.contributor.authorCorbet, EFen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorSöder, Ben_HK
dc.contributor.authorDavies, WIRen_HK
dc.date.accessioned2010-09-06T05:48:23Z-
dc.date.available2010-09-06T05:48:23Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Clinical Periodontology, 1999, v. 26 n. 8, p. 531-540en_HK
dc.identifier.issn0303-6979en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66676-
dc.description.abstractThis study aimed to determine the association between the levels of granulocyte elastase and prostaglandin E2 (PGE2) in GCF, and the concomitant presence of periodontopathogens in untreated adult periodontitis (AP). GCF and subgingival plaque were sampled by paper strips and paper points respectively, from various periodontal sites in 16 AP subjects. Granulocyte elastase activity in GCF was analyzed with a low molecular weight substrate specific for granulocyte elastase, pGluProVal-pNA, and the maximal rate of elastase activity (MR-EA, mAbs/min/site) was calculated. PGE2 levels in GCF were determined by radio-immunoassay. 5 species-specific DNA probes were used to detect the presence of A. actinomycetemcomitans (A.a., ATCC 43718), B. forsythus (B.f., ATCC 43037), P. gingivalis (P.g., ATCC 33277), P. intermedia (P.i., ATCC 33563), and T. denticola (T.d., ATCC 35405), with a sensitivity of 103 cells/paper point. No A.a. was detectable from all sites sampled. The predominant combination of species detected was B.f., P.g., P.i. & T.d. and it was significantly higher at periodontitis sites (68%) than at healthy (7%) or gingivitis sites (29%) (p<0.05). Overall, MR-EA values were strongly correlated with PGE2 levels (r=0.655, p<0.001), especially at these periodontitis sites co-infected by B.f., P.g., P.i. & T.d. (r=0.722, p<0.001). The periodontitis sites co-infected by the 4 species were observable from 15 subjects. These sites were sub-grouped into 8 subjects with a high MR-EA and 7 subjects with a low MR-EA. The PGE2 levels in the high MR-EA group were significantly higher than in the low MR-EA group (p<0.05). No significant differences in clinical or bacterial data were found between the two groups. While within the high MR-EA group, similar results were found between the paired periodontitis sites in each subject with highest and lowest MR-EA values. This study shows that the local host response to bacterial challenge in untreated periodontal pockets is diverse in terms of the intensity of inflammatory response measured by granulocyte elastase and PGE2 levels in GCF. A more thorough evaluation of the risk for active periodontal disease may involve the combined approaches to the test of the dynamic bacteria-host relations. © Munksgaard, 1999.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPEen_HK
dc.relation.ispartofJournal of Clinical Periodontologyen_HK
dc.subject.meshActinobacillus actinomycetemcomitans - isolation & purificationen_HK
dc.subject.meshAdulten_HK
dc.subject.meshBacteroides - isolation & purificationen_HK
dc.subject.meshDNA, Bacterial - analysisen_HK
dc.subject.meshDinoprostone - analysis - metabolismen_HK
dc.subject.meshDisease Progressionen_HK
dc.subject.meshEcologyen_HK
dc.subject.meshGingival Crevicular Fluid - immunology - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLeukocyte Elastase - analysis - metabolismen_HK
dc.subject.meshLongitudinal Studiesen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPeriodontal Indexen_HK
dc.subject.meshPeriodontitis - enzymology - immunology - microbiologyen_HK
dc.subject.meshPorphyromonas gingivalis - isolation & purificationen_HK
dc.subject.meshPrevotella intermedia - isolation & purificationen_HK
dc.subject.meshStatistics, Nonparametricen_HK
dc.subject.meshTreponema - isolation & purificationen_HK
dc.titleGranulocyte elastase activity and PGE2 levels in gingival crevicular fluid in relation to the presence of subgingival periodontopathogens in subjects with untreated adult periodontitisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0303-6979&volume=26&issue=8&spage=531&epage=540&date=1999&atitle=Granulocyte+elastase+activity+and+PGE2+levels+in+gingival+crevicular+fluid+in+relation+to+the+presence+of+subgingival+periodontopathogens+in+subjects+with+untreated+adult+periodontitisen_HK
dc.identifier.emailJin, LJ:ljjin@hkucc.hku.hken_HK
dc.identifier.emailLeung, WK:ewkleung@hkucc.hku.hken_HK
dc.identifier.emailCorbet, EF:efcorbet@hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authorityJin, LJ=rp00028en_HK
dc.identifier.authorityLeung, WK=rp00019en_HK
dc.identifier.authorityCorbet, EF=rp00005en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10450814-
dc.identifier.scopuseid_2-s2.0-0033174439en_HK
dc.identifier.hkuros41757en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033174439&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue8en_HK
dc.identifier.spage531en_HK
dc.identifier.epage540en_HK
dc.identifier.isiWOS:000081598900007-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridJin, LJ=7403328850en_HK
dc.identifier.scopusauthoridSöder, PÖ=7006570984en_HK
dc.identifier.scopusauthoridLeung, WK=25224691800en_HK
dc.identifier.scopusauthoridCorbet, EF=35609873200en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridSöder, B=6701795462en_HK
dc.identifier.scopusauthoridDavies, WIR=7202338137en_HK

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