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Article: Reverse transcriptase polymerase chain reaction (RT-PCR) detection of HLP gene expression in Candida glabrata and its possible role in in vitro haemolysin production

TitleReverse transcriptase polymerase chain reaction (RT-PCR) detection of HLP gene expression in Candida glabrata and its possible role in in vitro haemolysin production
Authors
Issue Date2004
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMIS
Citation
Apmis, 2004, v. 112 n. 4-5, p. 283-290 How to Cite?
AbstractAlthough haemolysins are known to be putative virulence factors contributing to pathogenicity in Candida species, the haemolytic activity of Candida glabrata and its genetic expression is ill understood at present. Thus, we studied a total of 34 Candida glabrata isolates for their in vitro haemolytic activity using a previously described plate assay system. The mRNA expression of HLP, a putative haemolysin gene, of these isolates was also evaluated using a semi-quantitative, non-competitive RT-PCR assay. All 34 C. glabrata isolates exhibited both partial (α) and complete (β) haemolytic activity to varying degrees. In parallel with the haemolytic activity, all isolates were also positive for HLP mRNA expression. The expression levels of HLP mRNA (as relative units) ranged from 1.01 to 1.82, with a mean value of 1.32. On regression analysis of latter values and the haemolytic activity (in terms of the dimension of the haemolytic zone in the plate assay) of the C. glabrata isolates a highly significant positive correlation was noted (r=0.759, p<0.0001). Taken together, our data illustrate not only the phenotypic characteristics of haemolysin(s) and HLP expression of a battery of C. glabrata clinical isolates, but also, for the first time, evidence for a role of HLP in haemolysis.
Persistent Identifierhttp://hdl.handle.net/10722/66643
ISSN
2015 Impact Factor: 1.933
2015 SCImago Journal Rankings: 0.855
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLuo, Gen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorCheung, BPKen_HK
dc.contributor.authorTang, Gen_HK
dc.date.accessioned2010-09-06T05:48:05Z-
dc.date.available2010-09-06T05:48:05Z-
dc.date.issued2004en_HK
dc.identifier.citationApmis, 2004, v. 112 n. 4-5, p. 283-290en_HK
dc.identifier.issn0903-4641en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66643-
dc.description.abstractAlthough haemolysins are known to be putative virulence factors contributing to pathogenicity in Candida species, the haemolytic activity of Candida glabrata and its genetic expression is ill understood at present. Thus, we studied a total of 34 Candida glabrata isolates for their in vitro haemolytic activity using a previously described plate assay system. The mRNA expression of HLP, a putative haemolysin gene, of these isolates was also evaluated using a semi-quantitative, non-competitive RT-PCR assay. All 34 C. glabrata isolates exhibited both partial (α) and complete (β) haemolytic activity to varying degrees. In parallel with the haemolytic activity, all isolates were also positive for HLP mRNA expression. The expression levels of HLP mRNA (as relative units) ranged from 1.01 to 1.82, with a mean value of 1.32. On regression analysis of latter values and the haemolytic activity (in terms of the dimension of the haemolytic zone in the plate assay) of the C. glabrata isolates a highly significant positive correlation was noted (r=0.759, p<0.0001). Taken together, our data illustrate not only the phenotypic characteristics of haemolysin(s) and HLP expression of a battery of C. glabrata clinical isolates, but also, for the first time, evidence for a role of HLP in haemolysis.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMISen_HK
dc.relation.ispartofAPMISen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCanadaen_HK
dc.subject.meshCandida glabrata - genetics - isolation & purificationen_HK
dc.subject.meshDNA Primersen_HK
dc.subject.meshFungal Proteins - geneticsen_HK
dc.subject.meshGeographyen_HK
dc.subject.meshGreat Britainen_HK
dc.subject.meshHemolysin Proteins - geneticsen_HK
dc.subject.meshHemolysisen_HK
dc.subject.meshHumansen_HK
dc.subject.meshRNA, Fungal - geneticsen_HK
dc.subject.meshRNA, Messenger - geneticsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - methodsen_HK
dc.titleReverse transcriptase polymerase chain reaction (RT-PCR) detection of HLP gene expression in Candida glabrata and its possible role in in vitro haemolysin productionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0903-4641&volume=112&spage=283&epage=290&date=2004&atitle=Reverse+transcriptase+polymerase+chain+reaction+(RT-PCR)+detection+of+HLP+gene+expression+in+Candida+glabrata+and+its+possible+role+in+in+vitro+haemolysin+productionen_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-0463.2004.apm11204-0509.xen_HK
dc.identifier.pmid15233644-
dc.identifier.scopuseid_2-s2.0-3442881981en_HK
dc.identifier.hkuros89115en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-3442881981&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume112en_HK
dc.identifier.issue4-5en_HK
dc.identifier.spage283en_HK
dc.identifier.epage290en_HK
dc.identifier.isiWOS:000222916700009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLuo, G=55112399700en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridCheung, BPK=7103294773en_HK
dc.identifier.scopusauthoridTang, G=36882453100en_HK

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