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Article: Coaggregation profiles of the microflora from root surface caries lesions

TitleCoaggregation profiles of the microflora from root surface caries lesions
Authors
KeywordsBacterial coaggregation
Biofilms
Dental plaque
Microflora
Issue Date2005
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2005, v. 50 n. 1, p. 23-32 How to Cite?
AbstractBacterial coaggregation reactions between different species and the auto aggregation of the same species are associated with the initiation and development of dental plaque and biofilms. As no such data is available on isolates from root caries lesions, we evaluated, by a visual, semi-quantitative scoring system and a spectrophotometric, quantitative assay, the coaggregation of 22 different wild-type microbial species comprising ten bacterial genera and a single Candida spp. The quantitative coaggregation assay we used proved to be a more sensitive method than the semi-quantitative, visual evaluation as the results yielded the percent coaggregation. Fusobacterium nucleatum, Lactobacillus acidophilus, Streptococcus bovis II/2 and Gemella morbillorum were observed having higher degrees of autoaggregation than the other examined strains. Significant levels of inter-species coaggregation was seen between: (1) Actinomyces spp. and Veillonella spp.; (2) Actinomyces israelii and Peptostreptococcus prevotii; (3) Campylobacter gracilis and Actinomyces spp.; (4) Prevotella intermedia and nine different species; and (5) Fusobacterium nucleatum and six other species. The single Candida albicans isolate did not coaggregate to a significant extent with any of the 21 bacterial isolates studied. Scanning electron microscopy observation of the coaggregation interactions between bacterial pairs having strong coaggregation reactions revealed varying adhesive patterns. Our findings on coaggregation amongst these isolates imply existence of multiple interactions between the coaggregation-inducing bacterial species in root caries. In particular, Actinomyces spp., Veillonella spp., Prevotella spp. and Fusobacterium spp. appear to play a significant role in this context. © 2004 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/66639
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorShen, Sen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorYip, HKen_HK
dc.date.accessioned2010-09-06T05:48:03Z-
dc.date.available2010-09-06T05:48:03Z-
dc.date.issued2005en_HK
dc.identifier.citationArchives Of Oral Biology, 2005, v. 50 n. 1, p. 23-32en_HK
dc.identifier.issn0003-9969en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66639-
dc.description.abstractBacterial coaggregation reactions between different species and the auto aggregation of the same species are associated with the initiation and development of dental plaque and biofilms. As no such data is available on isolates from root caries lesions, we evaluated, by a visual, semi-quantitative scoring system and a spectrophotometric, quantitative assay, the coaggregation of 22 different wild-type microbial species comprising ten bacterial genera and a single Candida spp. The quantitative coaggregation assay we used proved to be a more sensitive method than the semi-quantitative, visual evaluation as the results yielded the percent coaggregation. Fusobacterium nucleatum, Lactobacillus acidophilus, Streptococcus bovis II/2 and Gemella morbillorum were observed having higher degrees of autoaggregation than the other examined strains. Significant levels of inter-species coaggregation was seen between: (1) Actinomyces spp. and Veillonella spp.; (2) Actinomyces israelii and Peptostreptococcus prevotii; (3) Campylobacter gracilis and Actinomyces spp.; (4) Prevotella intermedia and nine different species; and (5) Fusobacterium nucleatum and six other species. The single Candida albicans isolate did not coaggregate to a significant extent with any of the 21 bacterial isolates studied. Scanning electron microscopy observation of the coaggregation interactions between bacterial pairs having strong coaggregation reactions revealed varying adhesive patterns. Our findings on coaggregation amongst these isolates imply existence of multiple interactions between the coaggregation-inducing bacterial species in root caries. In particular, Actinomyces spp., Veillonella spp., Prevotella spp. and Fusobacterium spp. appear to play a significant role in this context. © 2004 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_HK
dc.relation.ispartofArchives of Oral Biologyen_HK
dc.subjectBacterial coaggregationen_HK
dc.subjectBiofilmsen_HK
dc.subjectDental plaqueen_HK
dc.subjectMicrofloraen_HK
dc.subject.meshAgeden_HK
dc.subject.meshBacteria - isolation & purification - ultrastructureen_HK
dc.subject.meshBacterial Adhesion - physiologyen_HK
dc.subject.meshCandida albicans - isolation & purificationen_HK
dc.subject.meshColony Count, Microbial - methodsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMicroscopy, Electron, Scanning - methodsen_HK
dc.subject.meshRoot Caries - microbiologyen_HK
dc.subject.meshSpectrophotometry - methodsen_HK
dc.subject.meshStreptococcus - isolation & purification - ultrastructureen_HK
dc.titleCoaggregation profiles of the microflora from root surface caries lesionsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-9969&volume=50&spage=23&epage=32&date=2005&atitle=Coaggregation+profiles+of+the+microflora+from+root+surface+caries+lesionsen_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_HK
dc.identifier.emailYip, HK: kevin.h.k.yip@hkusua.hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authorityYip, HK=rp00027en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.archoralbio.2004.07.002en_HK
dc.identifier.pmid15598414-
dc.identifier.scopuseid_2-s2.0-10344225686en_HK
dc.identifier.hkuros98182en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-10344225686&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume50en_HK
dc.identifier.issue1en_HK
dc.identifier.spage23en_HK
dc.identifier.epage32en_HK
dc.identifier.isiWOS:000226459500004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridShen, S=7403431743en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridYip, HK=25423244900en_HK

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