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Article: The effect of limited exposure to antifungal agents on the germ tube formation of oral Candida albicans

TitleThe effect of limited exposure to antifungal agents on the germ tube formation of oral Candida albicans
Authors
Issue Date1998
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPM
Citation
Journal Of Oral Pathology And Medicine, 1998, v. 27 n. 5, p. 213-219 How to Cite?
AbstractCandidal adherence has been implicated as the first step in the pathogenesis of oral candidosis, and germ tube formation by Candida albicans has been attributed as a co-factor that promotes adherence. Oral candidosis is treated with polyenes and the azole group of antifungal agents. As the intraoral concentrations of antifungals fluctuate considerably due to the dynamics of the oral cavity, we investigated the effect of short exposure to sub-lethal concentrations of antifungals on the germ tube formation of Candida albicans. After determining the minimum inhibitory concentration (MIC) of the antifungal agents, ten oral isolates of Candida albicans were exposed to sub-lethal concentrations of nystatin (6xMIC), amphotericin B (8xMIC), 5-fluorocytosine (8xMIC), ketoconazole (4xMIC) and fluconazole (4xMIC), for 1 h. Following removal of the antifungal agent and subsequent incubation in a germ tube-inducing medium, the germ tube formation of these isolates was quantified. When compared with the controls, exposure to nystatin and amphotericin B almost completely inhibited germ tube formation of all the isolates (mean percentage reduction of 97.68 and 97.52%, respectively; P<0.0001), while ketoconazole suppressed this activity to a lesser degree (30.84%; P=0.0174). However, 5-fluorocytosine- and fluconazole-mediated germ tube suppression was minimal (12.63 and 15.93%, respectively; P=0.3255 and P=0.3791). In clinical terms, these findings indicate that short exposure to sub-therapeutic levels of commonly prescribed antifungals may modulate candidal germ tube formation, and thereby the clearance of the organisms from the oral cavity.
Persistent Identifierhttp://hdl.handle.net/10722/66631
ISSN
2015 Impact Factor: 1.859
2015 SCImago Journal Rankings: 0.731
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorEllepola, ANBen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:47:59Z-
dc.date.available2010-09-06T05:47:59Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Oral Pathology And Medicine, 1998, v. 27 n. 5, p. 213-219en_HK
dc.identifier.issn0904-2512en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66631-
dc.description.abstractCandidal adherence has been implicated as the first step in the pathogenesis of oral candidosis, and germ tube formation by Candida albicans has been attributed as a co-factor that promotes adherence. Oral candidosis is treated with polyenes and the azole group of antifungal agents. As the intraoral concentrations of antifungals fluctuate considerably due to the dynamics of the oral cavity, we investigated the effect of short exposure to sub-lethal concentrations of antifungals on the germ tube formation of Candida albicans. After determining the minimum inhibitory concentration (MIC) of the antifungal agents, ten oral isolates of Candida albicans were exposed to sub-lethal concentrations of nystatin (6xMIC), amphotericin B (8xMIC), 5-fluorocytosine (8xMIC), ketoconazole (4xMIC) and fluconazole (4xMIC), for 1 h. Following removal of the antifungal agent and subsequent incubation in a germ tube-inducing medium, the germ tube formation of these isolates was quantified. When compared with the controls, exposure to nystatin and amphotericin B almost completely inhibited germ tube formation of all the isolates (mean percentage reduction of 97.68 and 97.52%, respectively; P<0.0001), while ketoconazole suppressed this activity to a lesser degree (30.84%; P=0.0174). However, 5-fluorocytosine- and fluconazole-mediated germ tube suppression was minimal (12.63 and 15.93%, respectively; P=0.3255 and P=0.3791). In clinical terms, these findings indicate that short exposure to sub-therapeutic levels of commonly prescribed antifungals may modulate candidal germ tube formation, and thereby the clearance of the organisms from the oral cavity.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPMen_HK
dc.relation.ispartofJournal of Oral Pathology and Medicineen_HK
dc.subject.meshAIDS-Related Opportunistic Infections - microbiologyen_HK
dc.subject.meshAdhesivenessen_HK
dc.subject.meshAmphotericin B - administration & dosage - pharmacologyen_HK
dc.subject.meshAntifungal Agents - administration & dosage - pharmacologyen_HK
dc.subject.meshCandida albicans - cytology - drug effectsen_HK
dc.subject.meshCandidiasis, Oral - microbiologyen_HK
dc.subject.meshCulture Mediaen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshFluconazole - administration & dosage - pharmacologyen_HK
dc.subject.meshFlucytosine - administration & dosage - pharmacologyen_HK
dc.subject.meshHIV Seronegativityen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKetoconazole - administration & dosage - pharmacologyen_HK
dc.subject.meshMicroscopy, Electron, Scanningen_HK
dc.subject.meshMouth - microbiologyen_HK
dc.subject.meshNystatin - administration & dosage - pharmacologyen_HK
dc.titleThe effect of limited exposure to antifungal agents on the germ tube formation of oral Candida albicansen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0904-2512&volume=27&spage=213&epage=219&date=1998&atitle=The+effect+of+limited+exposure+to+antifungal+agents+on+the+germ+tube+formation+of+oral+Candida+albicansen_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid9682984-
dc.identifier.scopuseid_2-s2.0-0032079012en_HK
dc.identifier.hkuros31950en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032079012&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume27en_HK
dc.identifier.issue5en_HK
dc.identifier.spage213en_HK
dc.identifier.epage219en_HK
dc.identifier.isiWOS:000074237200005-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridEllepola, ANB=6604060863en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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