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Article: Commercial DNA Probes Reveal Colonization Patterns of Periodontopathogens in Chronic Periodontitis

TitleCommercial DNA Probes Reveal Colonization Patterns of Periodontopathogens in Chronic Periodontitis
Authors
KeywordsDental plaque - microbiology
Periodontitis - microbiology
Periodontitis - therapy
Statistical model
Issue Date2006
PublisherQuintessence Publishing Co Ltd. The Journal's web site is located at http://www.quintessencepublishing.co.uk/catalog/product_info.php?cPath=3&products_id=237
Citation
Perio: periodontal practice today, 2006, v. 3 n. 2, p. 87-96 How to Cite?
AbstractIn the present study, detection patterns of subgingival Porphyromonas gingivalis (Pg), Prevotella intermedia/nigrescens (Pi/n), Tannerella forsythia (Tf) and Treponema denticola (Td) were analysed, using commercially available species-specific DNA probes (DMDX/PathoTek, Switzerland) in 16 untreated periodontitis patients (5-7 non-adjacent sites per subject) before and one month after mechanical non-surgical periodontal therapy. The detection patterns of the pathogens at the two time points were studied using configural frequency analysis (CFA) and Stuart-Maxwell χ2 test. All subjects showed favourable clinical treatment responses. Prevalence of all microbes followed was significantly reduced after treatment. CFA identified: (i) two statistically significant configural types at both time points: co-detection of Pg, Pi/n, Tf, Td, and none of the four pathogens detectable (Holm's criteria p = 0.003); and (ii) one statistically significant configural anti-type post-treatment: reduced detection pattern of Pi/n alone (Holm's criteria p = 0.004). For many of the pre-treatment sites with Tf, Pg, Pi/n, and Td configural type, all these periodontopathogens were undetectable one month after treatment (p < 0.001, McNemar's post hoc analysis with Bonferroni adjustment). Within the limitations of this study, the results indicated a co-detection of subgingival periodontopathogens Pg, Pi/n, Tf, and Td. Analysis of the subgingival colonisation of these microbes might be a useful adjunct in the assessment of periodontal therapeutic outcomes.
Persistent Identifierhttp://hdl.handle.net/10722/66626
ISSN

 

DC FieldValueLanguage
dc.contributor.authorLeung, WKen_HK
dc.contributor.authorJin, Len_HK
dc.contributor.authorSöder, Ben_HK
dc.contributor.authorCorbet, EFen_HK
dc.date.accessioned2010-09-06T05:47:57Z-
dc.date.available2010-09-06T05:47:57Z-
dc.date.issued2006en_HK
dc.identifier.citationPerio: periodontal practice today, 2006, v. 3 n. 2, p. 87-96en_HK
dc.identifier.issn1742-3279en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66626-
dc.description.abstractIn the present study, detection patterns of subgingival Porphyromonas gingivalis (Pg), Prevotella intermedia/nigrescens (Pi/n), Tannerella forsythia (Tf) and Treponema denticola (Td) were analysed, using commercially available species-specific DNA probes (DMDX/PathoTek, Switzerland) in 16 untreated periodontitis patients (5-7 non-adjacent sites per subject) before and one month after mechanical non-surgical periodontal therapy. The detection patterns of the pathogens at the two time points were studied using configural frequency analysis (CFA) and Stuart-Maxwell χ2 test. All subjects showed favourable clinical treatment responses. Prevalence of all microbes followed was significantly reduced after treatment. CFA identified: (i) two statistically significant configural types at both time points: co-detection of Pg, Pi/n, Tf, Td, and none of the four pathogens detectable (Holm's criteria p = 0.003); and (ii) one statistically significant configural anti-type post-treatment: reduced detection pattern of Pi/n alone (Holm's criteria p = 0.004). For many of the pre-treatment sites with Tf, Pg, Pi/n, and Td configural type, all these periodontopathogens were undetectable one month after treatment (p < 0.001, McNemar's post hoc analysis with Bonferroni adjustment). Within the limitations of this study, the results indicated a co-detection of subgingival periodontopathogens Pg, Pi/n, Tf, and Td. Analysis of the subgingival colonisation of these microbes might be a useful adjunct in the assessment of periodontal therapeutic outcomes.-
dc.languageengen_HK
dc.publisherQuintessence Publishing Co Ltd. The Journal's web site is located at http://www.quintessencepublishing.co.uk/catalog/product_info.php?cPath=3&products_id=237en_HK
dc.relation.ispartofPerio: periodontal practice todayen_HK
dc.subjectDental plaque - microbiology-
dc.subjectPeriodontitis - microbiology-
dc.subjectPeriodontitis - therapy-
dc.subjectStatistical model-
dc.titleCommercial DNA Probes Reveal Colonization Patterns of Periodontopathogens in Chronic Periodontitisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1742-3279&volume=3&spage=87&epage=96&date=2006&atitle=Commercial+DNA+probes+reveal+colonization+patterns+of+periodontopathogens+in+chronic+periodontitisen_HK
dc.identifier.emailLeung, WK: ewkleung@hkucc.hku.hken_HK
dc.identifier.emailJin, L: ljjin@hkucc.hku.hken_HK
dc.identifier.emailCorbet, EF: efcorbet@hku.hken_HK
dc.identifier.authorityLeung, WK=rp00019en_HK
dc.identifier.authorityJin, LJ=rp00028en_HK
dc.identifier.authorityCorbet, EF=rp00005en_HK
dc.identifier.hkuros116421en_HK
dc.identifier.volume3-
dc.identifier.issue2-
dc.identifier.spage87-
dc.identifier.epage96-
dc.publisher.placeUnited Kingdom-

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