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Article: Differential modulation of human β-defensins expression in human gingival epithelia by Porphyromonas gingivalis lipopolysaccharide with tetra- And penta-acylated lipid A structures
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TitleDifferential modulation of human β-defensins expression in human gingival epithelia by Porphyromonas gingivalis lipopolysaccharide with tetra- And penta-acylated lipid A structures
 
AuthorsLu, Q1
Darveau, RP3
Samaranayake, LP1
Wang, CY2
Jin, L1
 
Issue Date2009
 
PublisherSage Publications Ltd.. The Journal's web site is located at http://ini.sagepub.com
 
CitationInnate Immunity, 2009, v. 15 n. 6, p. 325-335 [How to Cite?]
DOI: http://dx.doi.org/10.1177/1753425909104899
 
AbstractPorphyromonas gingivalis lipopolysaccharide (LPS) is a crucial virulence factor strongly involved in the development of chronic periodontitis. It displays a significant amount of lipid A structural heterogeneity, containing both tetra- (LPS 1435/1449) and penta-acylated (LPSi690) lipid A structures with opposing effects on E-selectin expression in human endothelial cells. Little is known about how these two isoforms of P. gingivalis LPS could differentially affect host innate immune responses in human gingival epithelia. The present study compares the modulatory effects of P. gingivalis LPS 1435/1449 and LPSi 690 on the expression of human β-defensins (hBDs) in the reconstituted human gingival epithelium, and examines the involvements of a panel of pattern recognition receptors in the modulatory effects concerned. It is shown that hBD-1, hBD-2 and hBD-3 mRNAs are significantly up-regulated by P. gingivalis LPS1690, but down-regulated by P. gingivalis LPS1435/1449. Toll-like receptor (TLR) 2 and CD14 mRNAs are also differentially regulated, and the modulation of hBD-2 expression may be through the co-operation of both TLR2 and TLR4. This study suggests that P. gingivalis LPS with different lipid A structures could differentially modulate host innate immune responses in human gingival epithelia, which may be a hitherto undescribed novel pathogenic mechanism of P. gingivalis in periodontal pathogenesis. © SAGE Publications 2009.
 
ISSN1753-4259
2013 Impact Factor: 2.459
 
DOIhttp://dx.doi.org/10.1177/1753425909104899
 
ISI Accession Number IDWOS:000272623500001
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
The University of Hong Kong
Funding Information:

This work was supported by grants from the Hong Kong Research Grants Council (HKU 7518/05M) and The University of Hong Kong (HKU CRCG 2007-2009) to LJJ.

 
ReferencesReferences in Scopus
 
GrantsNovel molecular mechanisms of innate host defense - implications in periodontal health and disease
 
DC FieldValue
dc.contributor.authorLu, Q
 
dc.contributor.authorDarveau, RP
 
dc.contributor.authorSamaranayake, LP
 
dc.contributor.authorWang, CY
 
dc.contributor.authorJin, L
 
dc.date.accessioned2010-09-06T05:47:54Z
 
dc.date.available2010-09-06T05:47:54Z
 
dc.date.issued2009
 
dc.description.abstractPorphyromonas gingivalis lipopolysaccharide (LPS) is a crucial virulence factor strongly involved in the development of chronic periodontitis. It displays a significant amount of lipid A structural heterogeneity, containing both tetra- (LPS 1435/1449) and penta-acylated (LPSi690) lipid A structures with opposing effects on E-selectin expression in human endothelial cells. Little is known about how these two isoforms of P. gingivalis LPS could differentially affect host innate immune responses in human gingival epithelia. The present study compares the modulatory effects of P. gingivalis LPS 1435/1449 and LPSi 690 on the expression of human β-defensins (hBDs) in the reconstituted human gingival epithelium, and examines the involvements of a panel of pattern recognition receptors in the modulatory effects concerned. It is shown that hBD-1, hBD-2 and hBD-3 mRNAs are significantly up-regulated by P. gingivalis LPS1690, but down-regulated by P. gingivalis LPS1435/1449. Toll-like receptor (TLR) 2 and CD14 mRNAs are also differentially regulated, and the modulation of hBD-2 expression may be through the co-operation of both TLR2 and TLR4. This study suggests that P. gingivalis LPS with different lipid A structures could differentially modulate host innate immune responses in human gingival epithelia, which may be a hitherto undescribed novel pathogenic mechanism of P. gingivalis in periodontal pathogenesis. © SAGE Publications 2009.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationInnate Immunity, 2009, v. 15 n. 6, p. 325-335 [How to Cite?]
DOI: http://dx.doi.org/10.1177/1753425909104899
 
dc.identifier.doihttp://dx.doi.org/10.1177/1753425909104899
 
dc.identifier.eissn1753-4267
 
dc.identifier.epage335
 
dc.identifier.hkuros168449
 
dc.identifier.isiWOS:000272623500001
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
The University of Hong Kong
Funding Information:

This work was supported by grants from the Hong Kong Research Grants Council (HKU 7518/05M) and The University of Hong Kong (HKU CRCG 2007-2009) to LJJ.

 
dc.identifier.issn1753-4259
2013 Impact Factor: 2.459
 
dc.identifier.issue6
 
dc.identifier.pmid19675119
 
dc.identifier.scopuseid_2-s2.0-74549193899
 
dc.identifier.spage325
 
dc.identifier.urihttp://hdl.handle.net/10722/66619
 
dc.identifier.volume15
 
dc.languageeng
 
dc.publisherSage Publications Ltd.. The Journal's web site is located at http://ini.sagepub.com
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofInnate Immunity
 
dc.relation.projectNovel molecular mechanisms of innate host defense - implications in periodontal health and disease
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAcylation
 
dc.subject.meshAntigens, CD14 - biosynthesis - genetics
 
dc.subject.meshBacteroidaceae Infections - complications - immunology
 
dc.subject.meshChronic Periodontitis - etiology - immunology
 
dc.subject.meshEpithelium - drug effects - immunology - metabolism - pathology
 
dc.subject.meshGene Expression Regulation - drug effects - immunology
 
dc.subject.meshGingiva - pathology
 
dc.subject.meshHost-Pathogen Interactions
 
dc.subject.meshHumans
 
dc.subject.meshImmunity, Innate
 
dc.subject.meshLipid A - analogs & derivatives - pharmacology
 
dc.subject.meshPorphyromonas gingivalis - immunology - pathogenicity
 
dc.subject.meshToll-Like Receptor 2 - biosynthesis - genetics
 
dc.subject.meshToll-Like Receptor 4 - biosynthesis - genetics
 
dc.subject.meshbeta-Defensins - biosynthesis - genetics
 
dc.titleDifferential modulation of human β-defensins expression in human gingival epithelia by Porphyromonas gingivalis lipopolysaccharide with tetra- And penta-acylated lipid A structures
 
dc.typeArticle
 
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Author Affiliations
  1. Prince Philip Dental Hospital
  2. University of California, Los Angeles
  3. University of Washington Seattle