Article: Differential modulation of human β-defensins expression in human gingival epithelia by Porphyromonas gingivalis lipopolysaccharide with tetra- And penta-acylated lipid A structures
| Title | Differential modulation of human β-defensins expression in human gingival epithelia by Porphyromonas gingivalis lipopolysaccharide with tetra- And penta-acylated lipid A structures | ||||||
|---|---|---|---|---|---|---|---|
| Authors | Lu, Q1 Darveau, RP3 Samaranayake, LP1 Wang, CY2 Jin, L1 | ||||||
| Issue Date | 2009 | ||||||
| Publisher | Sage Publications Ltd.. The Journal's web site is located at http://ini.sagepub.com | ||||||
| Citation | Innate Immunity, 2009, v. 15 n. 6, p. 325-335 [How to Cite?] DOI: http://dx.doi.org/10.1177/1753425909104899 | ||||||
| Abstract | Porphyromonas gingivalis lipopolysaccharide (LPS) is a crucial virulence factor strongly involved in the development of chronic periodontitis. It displays a significant amount of lipid A structural heterogeneity, containing both tetra- (LPS 1435/1449) and penta-acylated (LPSi690) lipid A structures with opposing effects on E-selectin expression in human endothelial cells. Little is known about how these two isoforms of P. gingivalis LPS could differentially affect host innate immune responses in human gingival epithelia. The present study compares the modulatory effects of P. gingivalis LPS 1435/1449 and LPSi 690 on the expression of human β-defensins (hBDs) in the reconstituted human gingival epithelium, and examines the involvements of a panel of pattern recognition receptors in the modulatory effects concerned. It is shown that hBD-1, hBD-2 and hBD-3 mRNAs are significantly up-regulated by P. gingivalis LPS1690, but down-regulated by P. gingivalis LPS1435/1449. Toll-like receptor (TLR) 2 and CD14 mRNAs are also differentially regulated, and the modulation of hBD-2 expression may be through the co-operation of both TLR2 and TLR4. This study suggests that P. gingivalis LPS with different lipid A structures could differentially modulate host innate immune responses in human gingival epithelia, which may be a hitherto undescribed novel pathogenic mechanism of P. gingivalis in periodontal pathogenesis. © SAGE Publications 2009. | ||||||
| ISSN | 1753-4259 2011 Impact Factor: 4.0 2011 SCImago Journal Rankings: 0.251 | ||||||
| DOI | http://dx.doi.org/10.1177/1753425909104899 | ||||||
| ISI Accession Number ID | WOS:000272623500001
Funding Information: This work was supported by grants from the Hong Kong Research Grants Council (HKU 7518/05M) and The University of Hong Kong (HKU CRCG 2007-2009) to LJJ. | ||||||
| References | References in Scopus | ||||||
| Grants | Novel molecular mechanisms of innate host defense - implications in periodontal health and disease Novel molecular mechanisms of innate host defense - implications in periodontal health and disease |
| dc.contributor.author | Lu, Q | ||||||
|---|---|---|---|---|---|---|---|
| dc.contributor.author | Darveau, RP | ||||||
| dc.contributor.author | Samaranayake, LP | ||||||
| dc.contributor.author | Wang, CY | ||||||
| dc.contributor.author | Jin, L | ||||||
| dc.date.accessioned | 2010-09-06T05:47:54Z | ||||||
| dc.date.available | 2010-09-06T05:47:54Z | ||||||
| dc.date.issued | 2009 | ||||||
| dc.description.abstract | Porphyromonas gingivalis lipopolysaccharide (LPS) is a crucial virulence factor strongly involved in the development of chronic periodontitis. It displays a significant amount of lipid A structural heterogeneity, containing both tetra- (LPS 1435/1449) and penta-acylated (LPSi690) lipid A structures with opposing effects on E-selectin expression in human endothelial cells. Little is known about how these two isoforms of P. gingivalis LPS could differentially affect host innate immune responses in human gingival epithelia. The present study compares the modulatory effects of P. gingivalis LPS 1435/1449 and LPSi 690 on the expression of human β-defensins (hBDs) in the reconstituted human gingival epithelium, and examines the involvements of a panel of pattern recognition receptors in the modulatory effects concerned. It is shown that hBD-1, hBD-2 and hBD-3 mRNAs are significantly up-regulated by P. gingivalis LPS1690, but down-regulated by P. gingivalis LPS1435/1449. Toll-like receptor (TLR) 2 and CD14 mRNAs are also differentially regulated, and the modulation of hBD-2 expression may be through the co-operation of both TLR2 and TLR4. This study suggests that P. gingivalis LPS with different lipid A structures could differentially modulate host innate immune responses in human gingival epithelia, which may be a hitherto undescribed novel pathogenic mechanism of P. gingivalis in periodontal pathogenesis. © SAGE Publications 2009. | ||||||
| dc.description.grant | Novel molecular mechanisms of innate host defense - implications in periodontal health and disease | ||||||
| dc.description.grant | Novel molecular mechanisms of innate host defense - implications in periodontal health and disease | ||||||
| dc.description.grantcode | 28079 | ||||||
| dc.description.grantcode | 29685 | ||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||
| dc.identifier.citation | Innate Immunity, 2009, v. 15 n. 6, p. 325-335 [How to Cite?] DOI: http://dx.doi.org/10.1177/1753425909104899 | ||||||
| dc.identifier.doi | http://dx.doi.org/10.1177/1753425909104899 | ||||||
| dc.identifier.epage | 335 | ||||||
| dc.identifier.hkuros | 168449 | ||||||
| dc.identifier.isi | WOS:000272623500001
Funding Information: This work was supported by grants from the Hong Kong Research Grants Council (HKU 7518/05M) and The University of Hong Kong (HKU CRCG 2007-2009) to LJJ. | ||||||
| dc.identifier.issn | 1753-4259 2011 Impact Factor: 4.0 2011 SCImago Journal Rankings: 0.251 | ||||||
| dc.identifier.issue | 6 | ||||||
| dc.identifier.pmid | 19675119 | ||||||
| dc.identifier.scopus | eid_2-s2.0-74549193899 | ||||||
| dc.identifier.spage | 325 | ||||||
| dc.identifier.uri | http://hdl.handle.net/10722/66619 | ||||||
| dc.identifier.volume | 15 | ||||||
| dc.language | eng | ||||||
| dc.publisher | Sage Publications Ltd.. The Journal's web site is located at http://ini.sagepub.com | ||||||
| dc.publisher.place | United Kingdom | ||||||
| dc.relation.ispartof | Innate Immunity | ||||||
| dc.relation.references | References in Scopus | ||||||
| dc.subject.mesh | Acylation | ||||||
| dc.subject.mesh | Antigens, CD14 - biosynthesis - genetics | ||||||
| dc.subject.mesh | Bacteroidaceae Infections - complications - immunology | ||||||
| dc.subject.mesh | Chronic Periodontitis - etiology - immunology | ||||||
| dc.subject.mesh | Epithelium - drug effects - immunology - metabolism - pathology | ||||||
| dc.subject.mesh | Gene Expression Regulation - drug effects - immunology | ||||||
| dc.subject.mesh | Gingiva - pathology | ||||||
| dc.subject.mesh | Host-Pathogen Interactions | ||||||
| dc.subject.mesh | Humans | ||||||
| dc.subject.mesh | Immunity, Innate | ||||||
| dc.subject.mesh | Lipid A - analogs & derivatives - pharmacology | ||||||
| dc.subject.mesh | Porphyromonas gingivalis - immunology - pathogenicity | ||||||
| dc.subject.mesh | Toll-Like Receptor 2 - biosynthesis - genetics | ||||||
| dc.subject.mesh | Toll-Like Receptor 4 - biosynthesis - genetics | ||||||
| dc.subject.mesh | beta-Defensins - biosynthesis - genetics | ||||||
| dc.title | Differential modulation of human β-defensins expression in human gingival epithelia by Porphyromonas gingivalis lipopolysaccharide with tetra- And penta-acylated lipid A structures | ||||||
| dc.type | Article |
Author Affiliations
- Prince Philip Dental Hospital
- University of California, Los Angeles
- University of Washington Seattle