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Article: The effect of brief exposure to sub-therapeutic concentrations of chlorhexidine gluconate on the germ tube formation of oral Candida albicans and its relationship to post-antifungal effect

TitleThe effect of brief exposure to sub-therapeutic concentrations of chlorhexidine gluconate on the germ tube formation of oral Candida albicans and its relationship to post-antifungal effect
Authors
Issue Date2000
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1
Citation
Oral Diseases, 2000, v. 6 n. 3, p. 166-171 How to Cite?
AbstractOBJECTIVES: Adherence of Candida albicans has been implicated as the first step in the pathogenesis of oral candidosis, and germ tube formation a contributory attribute. Recently, these organisms have also been implicated in persistent apical periodontitis. Chlorhexidine gluconate is by far the commonest antiseptic mouth wash prescribed in dentistry. As the intraoral concentrations of this antiseptic fluctuate considerably due to the dynamics of the oral cavity, the main objective of this study was to investigate the effect of brief exposure to three different sub-therapeutic concentrations of chlorhexidine gluconate (0.005%, 0.0025% and 0.00125%) on the germ tube formation of C. albicans. These findings were then correlated with the chlorhexidine-induced post-antifungal effect (PAFE) values we obtained in a study using the identical organisms and experimental conditions. DESIGN: Ten oral isolates of C. albicans were exposed to three different concentrations of chlorhexidine gluconate for 30 min, the antiseptic removed, and the germ tube formation of these isolates quantified following subsequent incubation in a germ tube inducing medium. The PAFE was evaluated by turbidometric measurement of growth. RESULTS: When compared with the controls, exposure to 0.005%, 0.0025% and 0.00125% chlorhexidine gluconate suppressed the ability to form germ tubes by 81.23% (P < 0.01), 42.74% (P < 0.01) and 9.13% (P > 0.05), respectively, while eliciting a mean PAFE of 9.91 h, 1.65 h and 0.67 h respectively. On regression analysis a significant positive correlation was observed between these two parameters (P < 0.0001: r = 0.7325). CONCLUSIONS: Taken together, these findings imply that short exposure to sub-therapeutic levels of chlorhexidine gluconate may modulate candidal germ tube formation as well as its growth, thereby suppressing its pathogencity in vivo.
Persistent Identifierhttp://hdl.handle.net/10722/66576
ISSN
2015 Impact Factor: 2.0
2015 SCImago Journal Rankings: 0.828
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorEllepola, ANBen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:47:31Z-
dc.date.available2010-09-06T05:47:31Z-
dc.date.issued2000en_HK
dc.identifier.citationOral Diseases, 2000, v. 6 n. 3, p. 166-171en_HK
dc.identifier.issn1354-523Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/66576-
dc.description.abstractOBJECTIVES: Adherence of Candida albicans has been implicated as the first step in the pathogenesis of oral candidosis, and germ tube formation a contributory attribute. Recently, these organisms have also been implicated in persistent apical periodontitis. Chlorhexidine gluconate is by far the commonest antiseptic mouth wash prescribed in dentistry. As the intraoral concentrations of this antiseptic fluctuate considerably due to the dynamics of the oral cavity, the main objective of this study was to investigate the effect of brief exposure to three different sub-therapeutic concentrations of chlorhexidine gluconate (0.005%, 0.0025% and 0.00125%) on the germ tube formation of C. albicans. These findings were then correlated with the chlorhexidine-induced post-antifungal effect (PAFE) values we obtained in a study using the identical organisms and experimental conditions. DESIGN: Ten oral isolates of C. albicans were exposed to three different concentrations of chlorhexidine gluconate for 30 min, the antiseptic removed, and the germ tube formation of these isolates quantified following subsequent incubation in a germ tube inducing medium. The PAFE was evaluated by turbidometric measurement of growth. RESULTS: When compared with the controls, exposure to 0.005%, 0.0025% and 0.00125% chlorhexidine gluconate suppressed the ability to form germ tubes by 81.23% (P < 0.01), 42.74% (P < 0.01) and 9.13% (P > 0.05), respectively, while eliciting a mean PAFE of 9.91 h, 1.65 h and 0.67 h respectively. On regression analysis a significant positive correlation was observed between these two parameters (P < 0.0001: r = 0.7325). CONCLUSIONS: Taken together, these findings imply that short exposure to sub-therapeutic levels of chlorhexidine gluconate may modulate candidal germ tube formation as well as its growth, thereby suppressing its pathogencity in vivo.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=1354-523X&site=1en_HK
dc.relation.ispartofOral Diseasesen_HK
dc.subject.meshAnalysis of Varianceen_HK
dc.subject.meshAnti-Infective Agents, Local - administration & dosage - pharmacologyen_HK
dc.subject.meshAntifungal Agents - pharmacologyen_HK
dc.subject.meshCandida albicans - drug effects - growth & development - pathogenicityen_HK
dc.subject.meshChlorhexidine - administration & dosage - analogs & derivatives - pharmacologyen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMouth - microbiologyen_HK
dc.subject.meshNephelometry and Turbidimetryen_HK
dc.subject.meshRegression Analysisen_HK
dc.titleThe effect of brief exposure to sub-therapeutic concentrations of chlorhexidine gluconate on the germ tube formation of oral Candida albicans and its relationship to post-antifungal effecten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1354-523X&volume=6&spage=166&epage=171&date=2000&atitle=The+effect+of+brief+exposure+to+sub-therapeutic+concentrations+of+chlorhexidine+gluconate+on+the+germ+tube+formation+of+oral+Candida+albicans+and+its+relationship+to+post-antifungal+effecten_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10822360-
dc.identifier.scopuseid_2-s2.0-0034040506en_HK
dc.identifier.hkuros48773en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034040506&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue3en_HK
dc.identifier.spage166en_HK
dc.identifier.epage171en_HK
dc.identifier.isiWOS:000087404100006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridEllepola, ANB=6604060863en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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