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Article: Phenotypes and randomly amplified polymorphic DNA profiles of Candida albicans isolates from root canal infections in a Finnish population

TitlePhenotypes and randomly amplified polymorphic DNA profiles of Candida albicans isolates from root canal infections in a Finnish population
Authors
Issue Date2001
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/OMI
Citation
Oral Microbiology And Immunology, 2001, v. 16 n. 2, p. 106-112 How to Cite?
AbstractA total of thirty-seven Candida albicans isolates from root canal infections in a Finnish population were subtyped using phenotypic and genotypic methods. A previously described biotyping method based on the presence of five different enzymes, assimilation of eleven different carbohydrates and boric acid sensitivity of the yeasts was used to determine the phenotype. Commercially available API ZYM and API 20 C test kits were used to determine the presence of enzymes and assimilation of carbohydrates. The sensitivity of the isolates to boric acid was tested by their ability to grow on yeast-nitrogen-agar with incorporated boric acid (1.8 mg · ml-1). Combination of the tests revealed a total of 14 different phenotypes. The majority of the isolates, 26 strains, were classifiable into three major phenotypes: 16 isolates (43.2%) belonged to phenotype A1R, six (16.2%) to A1S and four (10.8%) to B1S. The remaining 11 phenotypes represented only a single isolate each. The randomly amplified polymorphic DNA profiles were used to determine the genotypes. For this purpose two different primers, RSD6 and RSD12 were used to develop a combination randomly amplified polymorphic DNA profile for each isolate. Altogether 31 genotypes were noted among the 37 isolates, of which only three pairs of isolates presented with congruent phenotypic and genotypic profiles. The heterogeneity of both the phenotypic and randomly amplified polymorphic DNA profiles of C. albicans isolates from root canal infections is akin to previous reports from other oral and non-oral sources in different geographic locales.
Persistent Identifierhttp://hdl.handle.net/10722/66573
ISSN
2011 Impact Factor: 2.807
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWaltimo, TMTen_HK
dc.contributor.authorDassanayake, RSen_HK
dc.contributor.authorØrstavik, Den_HK
dc.contributor.authorHaapasalo, MPPen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:47:30Z-
dc.date.available2010-09-06T05:47:30Z-
dc.date.issued2001en_HK
dc.identifier.citationOral Microbiology And Immunology, 2001, v. 16 n. 2, p. 106-112en_HK
dc.identifier.issn0902-0055en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66573-
dc.description.abstractA total of thirty-seven Candida albicans isolates from root canal infections in a Finnish population were subtyped using phenotypic and genotypic methods. A previously described biotyping method based on the presence of five different enzymes, assimilation of eleven different carbohydrates and boric acid sensitivity of the yeasts was used to determine the phenotype. Commercially available API ZYM and API 20 C test kits were used to determine the presence of enzymes and assimilation of carbohydrates. The sensitivity of the isolates to boric acid was tested by their ability to grow on yeast-nitrogen-agar with incorporated boric acid (1.8 mg · ml-1). Combination of the tests revealed a total of 14 different phenotypes. The majority of the isolates, 26 strains, were classifiable into three major phenotypes: 16 isolates (43.2%) belonged to phenotype A1R, six (16.2%) to A1S and four (10.8%) to B1S. The remaining 11 phenotypes represented only a single isolate each. The randomly amplified polymorphic DNA profiles were used to determine the genotypes. For this purpose two different primers, RSD6 and RSD12 were used to develop a combination randomly amplified polymorphic DNA profile for each isolate. Altogether 31 genotypes were noted among the 37 isolates, of which only three pairs of isolates presented with congruent phenotypic and genotypic profiles. The heterogeneity of both the phenotypic and randomly amplified polymorphic DNA profiles of C. albicans isolates from root canal infections is akin to previous reports from other oral and non-oral sources in different geographic locales.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/OMIen_HK
dc.relation.ispartofOral Microbiology and Immunologyen_HK
dc.subject.meshBacterial Typing Techniquesen_HK
dc.subject.meshCandida albicans - classification - enzymology - geneticsen_HK
dc.subject.meshDNA Fingerprintingen_HK
dc.subject.meshFinlanden_HK
dc.subject.meshGenotypeen_HK
dc.subject.meshHumansen_HK
dc.subject.meshPeriapical Periodontitis - microbiologyen_HK
dc.subject.meshPhenotypeen_HK
dc.subject.meshRandom Amplified Polymorphic DNA Techniqueen_HK
dc.titlePhenotypes and randomly amplified polymorphic DNA profiles of Candida albicans isolates from root canal infections in a Finnish populationen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0902-0055&volume=16&spage=106&epage=112&date=2001&atitle=Phenotypes+and+randomly+amplified+polymorphic+DNA+profiles+of+Candida+albicans+isolates+from+root+canal+infections+in+a+Finnish+populationen_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1034/j.1399-302X.2001.016002106.xen_HK
dc.identifier.pmid11240864en_HK
dc.identifier.scopuseid_2-s2.0-0035134603en_HK
dc.identifier.hkuros56570en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035134603&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue2en_HK
dc.identifier.spage106en_HK
dc.identifier.epage112en_HK
dc.identifier.isiWOS:000167037100007-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridWaltimo, TMT=7004089148en_HK
dc.identifier.scopusauthoridDassanayake, RS=6603321318en_HK
dc.identifier.scopusauthoridØrstavik, D=7004417532en_HK
dc.identifier.scopusauthoridHaapasalo, MPP=7003569249en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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