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Article: Differential phospholipase gene expression by Candida albicans in artificial media and cultured human oral epithelium

TitleDifferential phospholipase gene expression by Candida albicans in artificial media and cultured human oral epithelium
Authors
Issue Date2006
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMIS
Citation
Apmis, 2006, v. 114 n. 12, p. 857-866 How to Cite?
AbstractPhospholipases B1, B2, C and D of Candida albicans play a significant role in the host invasive process. Hence we evaluated the in vitro expression of PLB1, PLB2, PLC1 and PLD1 in phospholipase-positive (PL +) and -deficient (PL -) C. albicans isolates in egg yolk agar (EYA), yeast peptone dextrose broth (YPD), and in a model of oral candidiasis based on reconstituted human oral epithelium (RHOE). The growth of Candida was then determined in YPD and its cellular invasion was investigated using the RHOE model. The PL + group demonstrated PLB1, PLB2, PLC1 and PLD1 expression in both EYA and YPD, in contrast to the PL - group, which expressed only PLB2 and PLD1. Although PL + isolates grew profusely in the RHOE model, they expressed only PLB2, PLC1 and PLD1, and not PLB1. Gene expression investigations could not be carried out with PL - isolates due to their inability to grow in the RHOE model. Significant growth differences in YPD medium were also observed within the PL + and PL - groups. Taken together, these findings indicate that phospholipase gene expression in C. albicans is differentially affected by their growth milieu, and this in turn may modulate the disease outcomes in vivo. Copyright © Apmis 2006.
Persistent Identifierhttp://hdl.handle.net/10722/66550
ISSN
2015 Impact Factor: 1.933
2015 SCImago Journal Rankings: 0.855
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorDassanayake, RSen_HK
dc.contributor.authorCheung, BPKen_HK
dc.contributor.authorJayatilake, JAMSen_HK
dc.contributor.authorYeung, KWSen_HK
dc.contributor.authorYau, JYYen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:47:18Z-
dc.date.available2010-09-06T05:47:18Z-
dc.date.issued2006en_HK
dc.identifier.citationApmis, 2006, v. 114 n. 12, p. 857-866en_HK
dc.identifier.issn0903-4641en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66550-
dc.description.abstractPhospholipases B1, B2, C and D of Candida albicans play a significant role in the host invasive process. Hence we evaluated the in vitro expression of PLB1, PLB2, PLC1 and PLD1 in phospholipase-positive (PL +) and -deficient (PL -) C. albicans isolates in egg yolk agar (EYA), yeast peptone dextrose broth (YPD), and in a model of oral candidiasis based on reconstituted human oral epithelium (RHOE). The growth of Candida was then determined in YPD and its cellular invasion was investigated using the RHOE model. The PL + group demonstrated PLB1, PLB2, PLC1 and PLD1 expression in both EYA and YPD, in contrast to the PL - group, which expressed only PLB2 and PLD1. Although PL + isolates grew profusely in the RHOE model, they expressed only PLB2, PLC1 and PLD1, and not PLB1. Gene expression investigations could not be carried out with PL - isolates due to their inability to grow in the RHOE model. Significant growth differences in YPD medium were also observed within the PL + and PL - groups. Taken together, these findings indicate that phospholipase gene expression in C. albicans is differentially affected by their growth milieu, and this in turn may modulate the disease outcomes in vivo. Copyright © Apmis 2006.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMISen_HK
dc.relation.ispartofAPMISen_HK
dc.subject.meshCandida albicans - enzymology - genetics - growth & development - isolation & purificationen_HK
dc.subject.meshCandidiasis, Oral - microbiologyen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshGene Expression Regulation, Enzymologicen_HK
dc.subject.meshGene Expression Regulation, Fungalen_HK
dc.subject.meshHistocytochemistryen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIsoenzymes - biosynthesis - geneticsen_HK
dc.subject.meshKeratinocytesen_HK
dc.subject.meshPhospholipases - biosynthesis - geneticsen_HK
dc.subject.meshRNA, Fungal - chemistry - geneticsen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.titleDifferential phospholipase gene expression by Candida albicans in artificial media and cultured human oral epitheliumen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0903-4641&volume=114&spage=857&epage=66&date=2006&atitle=Differential+phospholipase+gene+expression+by+Candida+albicans+in+artificial+media+and+cultured+human+oral+epitheliumen_HK
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-0463.2006.apm_479.xen_HK
dc.identifier.pmid17207086-
dc.identifier.scopuseid_2-s2.0-33845720523en_HK
dc.identifier.hkuros125350en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33845720523&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume114en_HK
dc.identifier.issue12en_HK
dc.identifier.spage857en_HK
dc.identifier.epage866en_HK
dc.identifier.isiWOS:000242902800005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridDassanayake, RS=6603321318en_HK
dc.identifier.scopusauthoridCheung, BPK=7103294773en_HK
dc.identifier.scopusauthoridJayatilake, JAMS=8441671500en_HK
dc.identifier.scopusauthoridYeung, KWS=13304249300en_HK
dc.identifier.scopusauthoridYau, JYY=7102167568en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.citeulike999723-

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