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Article: Toll-like receptors 2 and 4 gene polymorphisms in a Chinese population with periodontitis

TitleToll-like receptors 2 and 4 gene polymorphisms in a Chinese population with periodontitis
Authors
Issue Date2008
PublisherQuintessence Publishing Co, Inc. The Journal's web site is located at http://www.quintpub.com
Citation
Quintessence International, 2008, v. 39 n. 3, p. 217-226 How to Cite?
AbstractObjectives: Polymorphisms for tolMike receptor (TLR) 2 gene (Arg677Trp, Arg753Gln) and TLR4 gene (Asp299Gly, Thr399lle) that are associated with impaired lipopolysaccharide (LPS) signal transduction have recently been described. The present study aimed to investigate the relationship between TLRs 2 and 4 gene polymorphisms and periodontitis in a Chinese population. Method and Materials: Forty patients with generalized aggressive periodontitis, 50 patients with chronic periodontitis, and 100 periodontally healthy controls were recruited. All these subjects were of Han Chinese ethnicity. Genomic DNA was extracted from whole-blood samples. TLRs 2 and 4 genes were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with respective resfriction endonucleases. The alleles were detected by Polyacrylamide gel electrophoresis and visualized with ethidium bromide. Results: Heterozygosity for the TLR2 Arg677Trp polymorphism was found in all subjects. The TLR2 Arg753Gln mutant allele was not found in periodontitis patients, while a heterozygous frequency of 6% (6 of 100) was detected in the controls. The TLR4 Asp299Gly and Thr399lle mutant alleles were not found in any of the subjects. Conclusion: TLR2 Arg753Gln polymorphism may not be associated with aggressive or chronic periodontitis in the Chinese population. The prevalence of TLR2 Arp677Trp polymorphism seemed to be rather high, while that of TLR4 Asp299Gly and Thr399lle polymorphisms seemed to be rather low in the Chinese population, which did not permit any conclusion regarding its effects on periodontitis.
Persistent Identifierhttp://hdl.handle.net/10722/66541
ISSN
2015 Impact Factor: 0.821
2015 SCImago Journal Rankings: 0.396
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhu, Gen_HK
dc.contributor.authorLi, Cen_HK
dc.contributor.authorCao, Zen_HK
dc.contributor.authorCorbet, EFen_HK
dc.contributor.authorJin, Len_HK
dc.date.accessioned2010-09-06T05:47:14Z-
dc.date.available2010-09-06T05:47:14Z-
dc.date.issued2008en_HK
dc.identifier.citationQuintessence International, 2008, v. 39 n. 3, p. 217-226en_HK
dc.identifier.issn0033-6572en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66541-
dc.description.abstractObjectives: Polymorphisms for tolMike receptor (TLR) 2 gene (Arg677Trp, Arg753Gln) and TLR4 gene (Asp299Gly, Thr399lle) that are associated with impaired lipopolysaccharide (LPS) signal transduction have recently been described. The present study aimed to investigate the relationship between TLRs 2 and 4 gene polymorphisms and periodontitis in a Chinese population. Method and Materials: Forty patients with generalized aggressive periodontitis, 50 patients with chronic periodontitis, and 100 periodontally healthy controls were recruited. All these subjects were of Han Chinese ethnicity. Genomic DNA was extracted from whole-blood samples. TLRs 2 and 4 genes were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) with respective resfriction endonucleases. The alleles were detected by Polyacrylamide gel electrophoresis and visualized with ethidium bromide. Results: Heterozygosity for the TLR2 Arg677Trp polymorphism was found in all subjects. The TLR2 Arg753Gln mutant allele was not found in periodontitis patients, while a heterozygous frequency of 6% (6 of 100) was detected in the controls. The TLR4 Asp299Gly and Thr399lle mutant alleles were not found in any of the subjects. Conclusion: TLR2 Arg753Gln polymorphism may not be associated with aggressive or chronic periodontitis in the Chinese population. The prevalence of TLR2 Arp677Trp polymorphism seemed to be rather high, while that of TLR4 Asp299Gly and Thr399lle polymorphisms seemed to be rather low in the Chinese population, which did not permit any conclusion regarding its effects on periodontitis.en_HK
dc.languageengen_HK
dc.publisherQuintessence Publishing Co, Inc. The Journal's web site is located at http://www.quintpub.comen_HK
dc.relation.ispartofQuintessence Internationalen_HK
dc.subject.meshAllelesen_HK
dc.subject.meshCase-Control Studiesen_HK
dc.subject.meshGene Frequencyen_HK
dc.subject.meshGenetic Predisposition to Diseaseen_HK
dc.subject.meshHumansen_HK
dc.subject.meshPeriodontitis - geneticsen_HK
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_HK
dc.subject.meshPolymorphism, Single Nucleotideen_HK
dc.subject.meshToll-Like Receptor 2 - geneticsen_HK
dc.subject.meshToll-Like Receptor 4 - geneticsen_HK
dc.titleToll-like receptors 2 and 4 gene polymorphisms in a Chinese population with periodontitisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0033-6572&volume=39&spage=217&epage=226&date=2008&atitle=Toll-like+receptors+2+and+4+gene+polymorphisms+in+a+Chinese+population+with+periodontitisen_HK
dc.identifier.emailCorbet, EF:efcorbet@hku.hken_HK
dc.identifier.emailJin, L:ljjin@hkucc.hku.hken_HK
dc.identifier.authorityCorbet, EF=rp00005en_HK
dc.identifier.authorityJin, L=rp00028en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid18618036-
dc.identifier.scopuseid_2-s2.0-38949214306en_HK
dc.identifier.hkuros148590en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-38949214306&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume39en_HK
dc.identifier.issue3en_HK
dc.identifier.spage217en_HK
dc.identifier.epage226en_HK
dc.identifier.isiWOS:000254802600005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhu, G=15751936200en_HK
dc.identifier.scopusauthoridLi, C=15728225200en_HK
dc.identifier.scopusauthoridCao, Z=8642062400en_HK
dc.identifier.scopusauthoridCorbet, EF=35609873200en_HK
dc.identifier.scopusauthoridJin, L=7403328850en_HK

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