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Article: Expression of p16 and CDk4 in oral premalignant lesions and oral squamous cell carcinomas: A semi-quantitative immunohistochemical study

TitleExpression of p16 and CDk4 in oral premalignant lesions and oral squamous cell carcinomas: A semi-quantitative immunohistochemical study
Authors
Issue Date1999
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPM
Citation
Journal Of Oral Pathology And Medicine, 1999, v. 28 n. 4, p. 158-164 How to Cite?
AbstractThe protein, p16, the product of cyclin-dependent kinase number 2 (CDKN2) gene, is one of the negative regulators of the cell cycle. CDK4, encoded by CDK4 gene, is the substrate of p16. We investigated the expression of p16 and CDK4 and their role in the progression of oral premalignant lesions (OPLs) and oral squamous cell carcinomas (OSCCs) in a Chinese cohort. A total of 74 samples were obtained from patients with hyperkeratosis (10), OPLs [30; mild (10), moderate (10) and severe (10) dysplastic lesions], OSCCs (15; 8 non-metastatic, 7 metastatic), and normal oral tissues (10), together with local lymph nodes (9) of patients with metastatic OSCCs. A labelled streptavidin biotin (LSAB) immunohistochemistry assay was performed and a semi-quantitative method was used to evaluate the staining intensity. The staining patterns of p16 and CDK4 were similar in all tissues and were located in both the nuclei and the cytoplasm. However, the antigen distribution characteristics and the degree of expression of both p16 and CDK4 varied at different developmental stages of the lesions. Hyperkeratotic and dysplastic lesions tended to display a progressively increasing degree of p16- and CDK4-positive nuclei as compared with normal tissues. Also, positive staining cytoplasm was highly evident in OSCCs with a very low nuclear staining (P<0.05). Forty-six of 56-, p16-positive cases were CDK4-positive, while only 6 were CDK4-positive but p16-negative, implying a high correlation between these parameters (r=0.813, P<0.001). This study indicates that the expression of p16 and CDK4 is intimately involved in the development of OPLs and OSCCs and the likely existence of a positive feedback loop between p16 and CDK4.
Persistent Identifierhttp://hdl.handle.net/10722/66376
ISSN
2015 Impact Factor: 1.859
2015 SCImago Journal Rankings: 0.731
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Qen_HK
dc.contributor.authorLuo, Gen_HK
dc.contributor.authorLi, Ben_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:45:51Z-
dc.date.available2010-09-06T05:45:51Z-
dc.date.issued1999en_HK
dc.identifier.citationJournal Of Oral Pathology And Medicine, 1999, v. 28 n. 4, p. 158-164en_HK
dc.identifier.issn0904-2512en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66376-
dc.description.abstractThe protein, p16, the product of cyclin-dependent kinase number 2 (CDKN2) gene, is one of the negative regulators of the cell cycle. CDK4, encoded by CDK4 gene, is the substrate of p16. We investigated the expression of p16 and CDK4 and their role in the progression of oral premalignant lesions (OPLs) and oral squamous cell carcinomas (OSCCs) in a Chinese cohort. A total of 74 samples were obtained from patients with hyperkeratosis (10), OPLs [30; mild (10), moderate (10) and severe (10) dysplastic lesions], OSCCs (15; 8 non-metastatic, 7 metastatic), and normal oral tissues (10), together with local lymph nodes (9) of patients with metastatic OSCCs. A labelled streptavidin biotin (LSAB) immunohistochemistry assay was performed and a semi-quantitative method was used to evaluate the staining intensity. The staining patterns of p16 and CDK4 were similar in all tissues and were located in both the nuclei and the cytoplasm. However, the antigen distribution characteristics and the degree of expression of both p16 and CDK4 varied at different developmental stages of the lesions. Hyperkeratotic and dysplastic lesions tended to display a progressively increasing degree of p16- and CDK4-positive nuclei as compared with normal tissues. Also, positive staining cytoplasm was highly evident in OSCCs with a very low nuclear staining (P<0.05). Forty-six of 56-, p16-positive cases were CDK4-positive, while only 6 were CDK4-positive but p16-negative, implying a high correlation between these parameters (r=0.813, P<0.001). This study indicates that the expression of p16 and CDK4 is intimately involved in the development of OPLs and OSCCs and the likely existence of a positive feedback loop between p16 and CDK4.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPMen_HK
dc.relation.ispartofJournal of Oral Pathology and Medicineen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAnalysis of Varianceen_HK
dc.subject.meshCarcinoma, Squamous Cell - chemistry - genetics - metabolismen_HK
dc.subject.meshCell Transformation, Neoplasticen_HK
dc.subject.meshChi-Square Distributionen_HK
dc.subject.meshCyclin-Dependent Kinase 4en_HK
dc.subject.meshCyclin-Dependent Kinase Inhibitor p16 - biosynthesisen_HK
dc.subject.meshCyclin-Dependent Kinases - antagonists & inhibitors - biosynthesisen_HK
dc.subject.meshDisease Progressionen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshLeukoplakia, Oral - chemistry - genetics - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshMouth Mucosa - chemistry - metabolismen_HK
dc.subject.meshMouth Neoplasms - chemistry - genetics - metabolismen_HK
dc.subject.meshParaffin Embeddingen_HK
dc.subject.meshProto-Oncogene Proteinsen_HK
dc.subject.meshRegression Analysisen_HK
dc.subject.meshStatistics, Nonparametricen_HK
dc.titleExpression of p16 and CDk4 in oral premalignant lesions and oral squamous cell carcinomas: A semi-quantitative immunohistochemical studyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0278-2391&volume=28&spage=158&epage=164&date=1999&atitle=Expression+of+p16+and+CDK4+in+oral+premalignant+lesions+and+oral+squamous+cell+carcinomas:+a+semi-quantitative+immunohistochemical+studyen_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10235368-
dc.identifier.scopuseid_2-s2.0-0033120477en_HK
dc.identifier.hkuros40397en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033120477&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue4en_HK
dc.identifier.spage158en_HK
dc.identifier.epage164en_HK
dc.identifier.isiWOS:000079724200003-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridChen, Q=16244214800en_HK
dc.identifier.scopusauthoridLuo, G=55112399500en_HK
dc.identifier.scopusauthoridLi, B=16202895000en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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