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Article: A new, semi-automated technique for quantification of Candida adherence to denture acrylic surfaces

TitleA new, semi-automated technique for quantification of Candida adherence to denture acrylic surfaces
Authors
Issue Date1999
PublisherWiley-Blackwell Verlag GmbH. The Journal's web site is located at http://www.blackwellpublishing.com/journals/MYC
Citation
Mycoses, 1999, v. 42 n. 4, p. 265-267 How to Cite?
AbstractAdherence of Candida species to various host surfaces is an important prerequisite for colonization and pathogenesis. Researchers have problems in quantifying candidal adherence to plastic surfaces (used for fabrication of prostheses) due to coaggregation of adherent yeasts and to the laborious, time-consuming method of microscopic quantification. We describe here, a semiautomated image analysis system which is superior to conventional visual light microscopic techniques of quantifying yeasts attached to solid substrates such as acrylic. The results demonstrated a significant positive correlation between the number of cells quantified by traditional light microscopy and the corresponding area obtained by the semi-automated image analysis system (r = 0.83, P < 0.02). The new system is simple, less laborious and is particularly useful when the adherent attributes of a large battery of yeasts are characterized.
Persistent Identifierhttp://hdl.handle.net/10722/66300
ISSN
2015 Impact Factor: 2.332
2015 SCImago Journal Rankings: 0.921
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPanagoda, GJen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:45:13Z-
dc.date.available2010-09-06T05:45:13Z-
dc.date.issued1999en_HK
dc.identifier.citationMycoses, 1999, v. 42 n. 4, p. 265-267en_HK
dc.identifier.issn0933-7407en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66300-
dc.description.abstractAdherence of Candida species to various host surfaces is an important prerequisite for colonization and pathogenesis. Researchers have problems in quantifying candidal adherence to plastic surfaces (used for fabrication of prostheses) due to coaggregation of adherent yeasts and to the laborious, time-consuming method of microscopic quantification. We describe here, a semiautomated image analysis system which is superior to conventional visual light microscopic techniques of quantifying yeasts attached to solid substrates such as acrylic. The results demonstrated a significant positive correlation between the number of cells quantified by traditional light microscopy and the corresponding area obtained by the semi-automated image analysis system (r = 0.83, P < 0.02). The new system is simple, less laborious and is particularly useful when the adherent attributes of a large battery of yeasts are characterized.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Verlag GmbH. The Journal's web site is located at http://www.blackwellpublishing.com/journals/MYCen_HK
dc.relation.ispartofMycosesen_HK
dc.subject.meshAcrylic Resins - metabolismen_HK
dc.subject.meshCandida - physiologyen_HK
dc.subject.meshCell Adhesionen_HK
dc.subject.meshColony Count, Microbialen_HK
dc.subject.meshDenturesen_HK
dc.titleA new, semi-automated technique for quantification of Candida adherence to denture acrylic surfacesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0933-7407&volume=42&spage=265&epage=267&date=1999&atitle=A+new,+semi-automated+technique+for+quantification+of+Candida+adherence+to+denture+acrylic+surfacesen_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1439-0507.1999.00456.xen_HK
dc.identifier.pmid10424094-
dc.identifier.scopuseid_2-s2.0-0032778724en_HK
dc.identifier.hkuros44475en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032778724&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume42en_HK
dc.identifier.issue4en_HK
dc.identifier.spage265en_HK
dc.identifier.epage267en_HK
dc.identifier.isiWOS:000081495700007-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridPanagoda, GJ=6507149433en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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