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Article: The adherence of oral isolates of Enterobacteriaceae to HeLa cells: An in vitro method using image analysis

TitleThe adherence of oral isolates of Enterobacteriaceae to HeLa cells: An in vitro method using image analysis
Authors
KeywordsAdherence
Enterobacteriaceae
HeLa cells
Image analysis
Issue Date1996
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMIS
Citation
Apmis, 1996, v. 104 n. 1, p. 39-46 How to Cite?
AbstractAn in vitro model and an image analysis were designed to improve on existing quantification methods in the assessment of the adherence of Enterobacteriaceae to human epithelial cell monolayers. Adherence to HeLa cell monolayers of three oral isolates and one type strain from each of four species of Enterobacteriaceae over two incubation time periods was examined. Correction for actual cell area and a cube root transformation of the data to stabilize variance were applied. While behaviour varied between strains within species, E. cloacae was the most, and K. pneumoniae the least, adherent species irrespective of the incubation period. Increasing the incubation period from 30 min to 60 min resulted in greater adherence for E. cloacae, E. coli, and C. freundii, but not K. pneumoniae strains. The method permits the reliable measurement and valid analysis of the adherence of Enterobacteriaceae to cultured epithelial cell monolayers.
Persistent Identifierhttp://hdl.handle.net/10722/66297
ISSN
2015 Impact Factor: 1.933
2015 SCImago Journal Rankings: 0.855
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSedgley, CMen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorDarvell, BWen_HK
dc.date.accessioned2010-09-06T05:45:11Z-
dc.date.available2010-09-06T05:45:11Z-
dc.date.issued1996en_HK
dc.identifier.citationApmis, 1996, v. 104 n. 1, p. 39-46en_HK
dc.identifier.issn0903-4641en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66297-
dc.description.abstractAn in vitro model and an image analysis were designed to improve on existing quantification methods in the assessment of the adherence of Enterobacteriaceae to human epithelial cell monolayers. Adherence to HeLa cell monolayers of three oral isolates and one type strain from each of four species of Enterobacteriaceae over two incubation time periods was examined. Correction for actual cell area and a cube root transformation of the data to stabilize variance were applied. While behaviour varied between strains within species, E. cloacae was the most, and K. pneumoniae the least, adherent species irrespective of the incubation period. Increasing the incubation period from 30 min to 60 min resulted in greater adherence for E. cloacae, E. coli, and C. freundii, but not K. pneumoniae strains. The method permits the reliable measurement and valid analysis of the adherence of Enterobacteriaceae to cultured epithelial cell monolayers.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/APMISen_HK
dc.relation.ispartofAPMISen_HK
dc.subjectAdherenceen_HK
dc.subjectEnterobacteriaceaeen_HK
dc.subjectHeLa cellsen_HK
dc.subjectImage analysisen_HK
dc.subject.meshCell Adhesion-
dc.subject.meshEnterobacteriaceae - cytology - isolation & purification-
dc.subject.meshHeLa Cells-
dc.subject.meshHumans-
dc.subject.meshImage Processing, Computer-Assisted-
dc.subject.meshMouth - microbiology-
dc.titleThe adherence of oral isolates of Enterobacteriaceae to HeLa cells: An in vitro method using image analysisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0903-4641&volume=104&spage=39&epage=46&date=1996&atitle=The+adherence+of+oral+isolates+of+enterobacteriaceae+to+HeLa+cellsen_HK
dc.identifier.emailSamaranayake, LP: lakshman@hku.hken_HK
dc.identifier.emailDarvell, BW: b.w.darvell@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authorityDarvell, BW=rp00007en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid8645457-
dc.identifier.scopuseid_2-s2.0-0029958782en_HK
dc.identifier.hkuros23383en_HK
dc.identifier.hkuros10500-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0029958782&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume104en_HK
dc.identifier.issue1en_HK
dc.identifier.spage39en_HK
dc.identifier.epage46en_HK
dc.identifier.isiWOS:A1996UA58700007-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSedgley, CM=6701569363en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridDarvell, BW=7005953926en_HK

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