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Article: Phospholipase B enzyme expression is not associated with other virulence attributes in Candida albicans isolates from patients with human immunodeficiency virus infection

TitlePhospholipase B enzyme expression is not associated with other virulence attributes in Candida albicans isolates from patients with human immunodeficiency virus infection
Authors
Issue Date2005
PublisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.org
Citation
Journal Of Medical Microbiology, 2005, v. 54 n. 6, p. 583-593 How to Cite?
AbstractThe extracellular phospholipases of Candida albicans are considered to play a significant role in the pathogenesis of human infections. Therefore 30 clinical isolates of C. albicans from human immunodeficiency virus (HIV)-infected individuals were screened for phospholipase production in vitro (using an egg-yolk-agar medium). Two groups of six isolates with positive (group A) or deficient (group B) phospholipase activity were then analysed for phospholipase B1 (PLB1) gene expression both in egg-yolk-agar and yeast extract/peptone/dextrose (YPD) broth media. A total of four virulence attributes of these two groups were in turn characterized, namely their germ-tube formation, cell-surface-hydrophobicity (CSH), adhesion to buccal epithelial cells (ABEC) and haemolysin production, and these factors were subsequently correlated with PLB1 expression. In the phospholipase-producing isolates (group A) a positive correlation was demonstrated between phospholipase production and the degree of PLB1 expression in YPD medium (r = 0.96, P < 0.01). No such association was observed in group A isolates for PLB1 expression in egg-yolk-agar medium. Further, PLB1 expression in egg-yolk agar was less than that in YPD medium, although a positive correlation was seen between the expression levels on regression analysis (r = 0.86, P = 0.026). Surprisingly, however, no significant associations were observed in either growth media between PLB1 expression and any of the four pathogenic attributes examined (P < 0.001). A significant correlation was seen between CSH and ABEC (r = 0.74) in group A isolates. The phospholipase-deficient group B, however, demonstrated a significant correlation between the latter parameters (r = +0.50) and also between germ-tube formation and ABEC (r = -0.59), and germ-tube formation and haemolysin production (r = +0.31). It appears that in oral C. albicans isolates in HIV infection there may be no significant association between the degree of PLB1 expression and other widely recognized major virulence attributes. © 2005 SGM.
Persistent Identifierhttp://hdl.handle.net/10722/66287
ISSN
2015 Impact Factor: 2.269
2015 SCImago Journal Rankings: 1.060
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorDassanayake, RSen_HK
dc.contributor.authorJayatilake, JAMSen_HK
dc.contributor.authorCheung, BPKen_HK
dc.contributor.authorYau, JYYen_HK
dc.contributor.authorYeung, KWSen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:45:07Z-
dc.date.available2010-09-06T05:45:07Z-
dc.date.issued2005en_HK
dc.identifier.citationJournal Of Medical Microbiology, 2005, v. 54 n. 6, p. 583-593en_HK
dc.identifier.issn0022-2615en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66287-
dc.description.abstractThe extracellular phospholipases of Candida albicans are considered to play a significant role in the pathogenesis of human infections. Therefore 30 clinical isolates of C. albicans from human immunodeficiency virus (HIV)-infected individuals were screened for phospholipase production in vitro (using an egg-yolk-agar medium). Two groups of six isolates with positive (group A) or deficient (group B) phospholipase activity were then analysed for phospholipase B1 (PLB1) gene expression both in egg-yolk-agar and yeast extract/peptone/dextrose (YPD) broth media. A total of four virulence attributes of these two groups were in turn characterized, namely their germ-tube formation, cell-surface-hydrophobicity (CSH), adhesion to buccal epithelial cells (ABEC) and haemolysin production, and these factors were subsequently correlated with PLB1 expression. In the phospholipase-producing isolates (group A) a positive correlation was demonstrated between phospholipase production and the degree of PLB1 expression in YPD medium (r = 0.96, P < 0.01). No such association was observed in group A isolates for PLB1 expression in egg-yolk-agar medium. Further, PLB1 expression in egg-yolk agar was less than that in YPD medium, although a positive correlation was seen between the expression levels on regression analysis (r = 0.86, P = 0.026). Surprisingly, however, no significant associations were observed in either growth media between PLB1 expression and any of the four pathogenic attributes examined (P < 0.001). A significant correlation was seen between CSH and ABEC (r = 0.74) in group A isolates. The phospholipase-deficient group B, however, demonstrated a significant correlation between the latter parameters (r = +0.50) and also between germ-tube formation and ABEC (r = -0.59), and germ-tube formation and haemolysin production (r = +0.31). It appears that in oral C. albicans isolates in HIV infection there may be no significant association between the degree of PLB1 expression and other widely recognized major virulence attributes. © 2005 SGM.en_HK
dc.languageengen_HK
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://jmm.sgmjournals.orgen_HK
dc.relation.ispartofJournal of Medical Microbiologyen_HK
dc.subject.meshBacterial Adhesionen_HK
dc.subject.meshCandida albicans - metabolism - pathogenicityen_HK
dc.subject.meshCandidiasis - complicationsen_HK
dc.subject.meshCulture Mediaen_HK
dc.subject.meshEpithelial Cells - metabolism - microbiologyen_HK
dc.subject.meshFungal Proteins - metabolismen_HK
dc.subject.meshHIV Infections - complicationsen_HK
dc.subject.meshHemolysin Proteins - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHydrophobic and Hydrophilic Interactionsen_HK
dc.subject.meshLysophospholipase - metabolismen_HK
dc.subject.meshMouth - metabolism - microbiologyen_HK
dc.subject.meshVirulence Factors - metabolismen_HK
dc.titlePhospholipase B enzyme expression is not associated with other virulence attributes in Candida albicans isolates from patients with human immunodeficiency virus infectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-2615&volume=54&spage=583&epage=593&date=2005&atitle=Phospholipase+B+enzyme+expression+is+not+associated+with+other+virulence+attributes+in+Candida+albicans+isolates+from+patients+with+human+immunodeficiency+virus+infectionen_HK
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1099/jmm.0.45762-0en_HK
dc.identifier.pmid15888468-
dc.identifier.scopuseid_2-s2.0-20344393366en_HK
dc.identifier.hkuros98129en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-20344393366&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume54en_HK
dc.identifier.issue6en_HK
dc.identifier.spage583en_HK
dc.identifier.epage593en_HK
dc.identifier.isiWOS:000231445800012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridDassanayake, RS=6603321318en_HK
dc.identifier.scopusauthoridJayatilake, JAMS=8441671500en_HK
dc.identifier.scopusauthoridCheung, BPK=7103294773en_HK
dc.identifier.scopusauthoridYau, JYY=7102167568en_HK
dc.identifier.scopusauthoridYeung, KWS=13304249300en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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