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Article: In vitro biofilm formation of Candida albicans and non-albicans Candida species under dynamic and anaerobic conditions

TitleIn vitro biofilm formation of Candida albicans and non-albicans Candida species under dynamic and anaerobic conditions
Authors
Issue Date2007
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2007, v. 52 n. 8, p. 761-767 How to Cite?
AbstractAn understanding of biofilm behavior of Candida species under different environmental conditions is key to the development of effective preventive measures for candidal infections. Hence in this study we assessed the impact of the environmental milieu on Candida biofilm formation using polystyrene, flat-bottomed 96-well microtiter plates. A total of 20, comprising 10 clinical isolates each of Candida albicans and, non-albicans species of Candida were compared for their biofilm forming ability both under aerobic and anaerobic conditions, and static and dynamic conditions. XTT reduction assay was used to quantify the sessile growth. Biofilm formation of all 10 C. albicans isolates differed significantly between dynamic and static states under both atmospheric conditions (P < 0.05). For non-albicans Candida species, a significant difference in biofilm growth between dynamic and static states was noted only when incubated aerobically (P < 0.05), and no significant difference in biofilm formation was noted between aerobic and anaerobic conditions. Scanning electron microscopy revealed that C. albicans produced a compact multilayered biofilm embedded in noticeably higher quantity of extracellular polymeric matrix in aerobic/dynamic conditions compared with anaerobic/static conditions. Our data indicate that biofilm formation of C. albicans and non-albicans Candida species is modulated by hydrodynamic conditions and ambient oxygen gradients. However, further work is required to fully elucidate how Candida biofilms persist within the oral milieu under such challenging ecological pressures. © 2007.
Persistent Identifierhttp://hdl.handle.net/10722/66264
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorThein, ZMen_HK
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:44:55Z-
dc.date.available2010-09-06T05:44:55Z-
dc.date.issued2007en_HK
dc.identifier.citationArchives Of Oral Biology, 2007, v. 52 n. 8, p. 761-767en_HK
dc.identifier.issn0003-9969en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66264-
dc.description.abstractAn understanding of biofilm behavior of Candida species under different environmental conditions is key to the development of effective preventive measures for candidal infections. Hence in this study we assessed the impact of the environmental milieu on Candida biofilm formation using polystyrene, flat-bottomed 96-well microtiter plates. A total of 20, comprising 10 clinical isolates each of Candida albicans and, non-albicans species of Candida were compared for their biofilm forming ability both under aerobic and anaerobic conditions, and static and dynamic conditions. XTT reduction assay was used to quantify the sessile growth. Biofilm formation of all 10 C. albicans isolates differed significantly between dynamic and static states under both atmospheric conditions (P < 0.05). For non-albicans Candida species, a significant difference in biofilm growth between dynamic and static states was noted only when incubated aerobically (P < 0.05), and no significant difference in biofilm formation was noted between aerobic and anaerobic conditions. Scanning electron microscopy revealed that C. albicans produced a compact multilayered biofilm embedded in noticeably higher quantity of extracellular polymeric matrix in aerobic/dynamic conditions compared with anaerobic/static conditions. Our data indicate that biofilm formation of C. albicans and non-albicans Candida species is modulated by hydrodynamic conditions and ambient oxygen gradients. However, further work is required to fully elucidate how Candida biofilms persist within the oral milieu under such challenging ecological pressures. © 2007.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_HK
dc.relation.ispartofArchives of Oral Biologyen_HK
dc.subject.meshAerobiosisen_HK
dc.subject.meshAnaerobiosisen_HK
dc.subject.meshBiofilms - growth & developmenten_HK
dc.subject.meshCandida - physiology - ultrastructureen_HK
dc.subject.meshCandida albicans - physiology - ultrastructureen_HK
dc.subject.meshCandida glabrata - physiology - ultrastructureen_HK
dc.subject.meshCandida tropicalis - physiology - ultrastructureen_HK
dc.subject.meshExtracellular Matrix - physiologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshIndicators and Reagentsen_HK
dc.subject.meshMicroscopy, Electron, Scanningen_HK
dc.subject.meshMycology - methodsen_HK
dc.subject.meshOxygen - pharmacologyen_HK
dc.subject.meshPolystyrenesen_HK
dc.subject.meshRheologyen_HK
dc.subject.meshTetrazolium Salts - diagnostic useen_HK
dc.titleIn vitro biofilm formation of Candida albicans and non-albicans Candida species under dynamic and anaerobic conditionsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-9969&volume=52&spage=761&epage=767&date=2007&atitle=In+vitro+biofilm+formation+of+Candida+albicans+and+non-albicans+Candida+species+under+dynamic+and+anaerobic+conditionsen_HK
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.archoralbio.2007.01.009en_HK
dc.identifier.pmid17321487-
dc.identifier.scopuseid_2-s2.0-34250340103en_HK
dc.identifier.hkuros128027en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34250340103&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue8en_HK
dc.identifier.spage761en_HK
dc.identifier.epage767en_HK
dc.identifier.isiWOS:000248111400009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridThein, ZM=14049367300en_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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