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Article: The effect of fluoridated and non-fluoridated rewetting agents on in vitro recurrent caries

TitleThe effect of fluoridated and non-fluoridated rewetting agents on in vitro recurrent caries
Authors
KeywordsFluoride rewetting agent
Microradiography
Polarised light microscopy
Recurrent caries
STEM/EDX
TEM
Issue Date2001
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent
Citation
Journal Of Dentistry, 2001, v. 29 n. 4, p. 255-273 How to Cite?
AbstractObjective: This study examined the in vitro caries inhibiting potential of fluoridated and non-fluoridated rewetting agents that are applied to acid-etched enamel and dentine before the use of a water-free, dentine adhesive. Materials and methods: Twelve caries-free premolars were divided into three groups of four teeth each. 2 × 3 × 1.5 mm cavities were prepared on the mesial and distal surfaces of each tooth, with half of the cavosurface margin in enamel and half in root dentine. In Group I (control), One-Step (Bisco, Schaumburg, USA) was applied without etching or rewetting agents. In Group II, cavities were acid-etched, rinsed, dried, and rewetted with Aqua-Prep (Bisco), a non-fluoridated rewetting agent, and then bonded with One-Step. Treatment for Group III was similar to Group II, except that Aqua-Prep F (Bisco), a fluoridated rewetting agent was used. Bonded cavities were restored with a non-fluoride-containing flowable composite (ÆliteFlo, Bisco). Artificial carious lesions were induced in these specimens, from which multiple 100 ± 20 μm thick longitudinal sections were prepared, yielding 16 specimens per group for evaluation with polarised light microscopy (PLM) and microradiography (MRG). Representative sections were processed for transmission electron microscopy (TEM) examination and scanning transmission electron microscopy/energy dispersive X-ray (STEM/EDX) analyses. Results: The differences in demineralisation of dentine among the groups were not statistically significant for 'relative' lesion depth (p > 0.05, ANOVA, Student-Neuman-Keuls test), but highly significant for 'relative' lesion area (p < 0.001). Wall lesions were consistently present in Group I, while inhibition zones were invariably observed in Group III. 87.5% of Group II specimens exhibited neither wall lesion nor inhibition zone. TEM showed that remnant dentine apatite crystallites within the inhibition zones in Group III were larger and denser than those present within the corresponding wall lesions. STEM/EDX analyses confirmed the presence of calcium, phosphorus and fluorine in these plate-like crystallites. Conclusion: When used with a water-free, single-bottle dentine adhesive, a non-fluoridated rewetting agent is able to reduce, but cannot completely prevent recurrent caries. The use of a fluoridated rewetting agent is useful under the situation when microleakage occurs, by providing the additional benefit of fluoride-induced demineralisation inhibition. © 2001 Elsevier Science Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/66218
ISSN
2015 Impact Factor: 3.109
2015 SCImago Journal Rankings: 1.029
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorItthagarun, Aen_HK
dc.contributor.authorKing, NMen_HK
dc.contributor.authorWefel, JSen_HK
dc.contributor.authorTay, FRen_HK
dc.contributor.authorPashley, DHen_HK
dc.date.accessioned2010-09-06T05:44:33Z-
dc.date.available2010-09-06T05:44:33Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Dentistry, 2001, v. 29 n. 4, p. 255-273en_HK
dc.identifier.issn0300-5712en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66218-
dc.description.abstractObjective: This study examined the in vitro caries inhibiting potential of fluoridated and non-fluoridated rewetting agents that are applied to acid-etched enamel and dentine before the use of a water-free, dentine adhesive. Materials and methods: Twelve caries-free premolars were divided into three groups of four teeth each. 2 × 3 × 1.5 mm cavities were prepared on the mesial and distal surfaces of each tooth, with half of the cavosurface margin in enamel and half in root dentine. In Group I (control), One-Step (Bisco, Schaumburg, USA) was applied without etching or rewetting agents. In Group II, cavities were acid-etched, rinsed, dried, and rewetted with Aqua-Prep (Bisco), a non-fluoridated rewetting agent, and then bonded with One-Step. Treatment for Group III was similar to Group II, except that Aqua-Prep F (Bisco), a fluoridated rewetting agent was used. Bonded cavities were restored with a non-fluoride-containing flowable composite (ÆliteFlo, Bisco). Artificial carious lesions were induced in these specimens, from which multiple 100 ± 20 μm thick longitudinal sections were prepared, yielding 16 specimens per group for evaluation with polarised light microscopy (PLM) and microradiography (MRG). Representative sections were processed for transmission electron microscopy (TEM) examination and scanning transmission electron microscopy/energy dispersive X-ray (STEM/EDX) analyses. Results: The differences in demineralisation of dentine among the groups were not statistically significant for 'relative' lesion depth (p > 0.05, ANOVA, Student-Neuman-Keuls test), but highly significant for 'relative' lesion area (p < 0.001). Wall lesions were consistently present in Group I, while inhibition zones were invariably observed in Group III. 87.5% of Group II specimens exhibited neither wall lesion nor inhibition zone. TEM showed that remnant dentine apatite crystallites within the inhibition zones in Group III were larger and denser than those present within the corresponding wall lesions. STEM/EDX analyses confirmed the presence of calcium, phosphorus and fluorine in these plate-like crystallites. Conclusion: When used with a water-free, single-bottle dentine adhesive, a non-fluoridated rewetting agent is able to reduce, but cannot completely prevent recurrent caries. The use of a fluoridated rewetting agent is useful under the situation when microleakage occurs, by providing the additional benefit of fluoride-induced demineralisation inhibition. © 2001 Elsevier Science Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdenten_HK
dc.relation.ispartofJournal of Dentistryen_HK
dc.rightsJournal of Dentistry. Copyright © Elsevier Ltd.en_HK
dc.subjectFluoride rewetting agenten_HK
dc.subjectMicroradiographyen_HK
dc.subjectPolarised light microscopyen_HK
dc.subjectRecurrent cariesen_HK
dc.subjectSTEM/EDXen_HK
dc.subjectTEMen_HK
dc.titleThe effect of fluoridated and non-fluoridated rewetting agents on in vitro recurrent cariesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-5712&volume=29&spage=255&epage=273&date=2001&atitle=The+effect+of+fluoridated+and+non-fluoridated+rewetting+agents+on+in+vitro+recurrent+cariesen_HK
dc.identifier.emailKing, NM: hhdbknm@hkucc.hku.hken_HK
dc.identifier.authorityKing, NM=rp00006en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0300-5712(01)00014-8en_HK
dc.identifier.pmid11525227-
dc.identifier.scopuseid_2-s2.0-0035346347en_HK
dc.identifier.hkuros57469en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035346347&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue4en_HK
dc.identifier.spage255en_HK
dc.identifier.epage273en_HK
dc.identifier.isiWOS:000169030900004-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridItthagarun, A=6701591745en_HK
dc.identifier.scopusauthoridKing, NM=7201762850en_HK
dc.identifier.scopusauthoridWefel, JS=7006428745en_HK
dc.identifier.scopusauthoridTay, FR=7102091962en_HK
dc.identifier.scopusauthoridPashley, DH=35448600800en_HK

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