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Article: The interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and disease

TitleThe interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and disease
Authors
Issue Date2009
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
Citation
Journal Of Clinical Periodontology, 2009, v. 36 n. 8, p. 619-626 How to Cite?
AbstractAim: Periodontal pathogenesis is characterized by Gram-negative bacteria activation of series of pro- and anti-inflammatory cytokines from host cells through the pathway of lipopolysaccharide (LPS), LPS-binding protein (LBP) and CD14. The present study investigated the expression profiles of interleukin (IL)-1β and IL-10 in periodontal health and disease, and examined the effects of Escherichia coli LPS and LBP interaction on the expression of IL-1β and IL-10 by human gingival fibroblasts (HGF). Material and Methods: Gingival biopsies were collected from 44 subjects with chronic periodontitis and 15 periodontally healthy subjects. The expression of IL-1β and IL-10 was detected by immunohistochemistry. The mRNA expression of IL-1β and IL-10 in HGF was detected by RT-PCR with or without recombinant human LBP (rhLBP), while the peptides were analysed by an enzyme-linked immunosorbent assay. Results: IL-1β was detected in both oral sulcular epithelia of healthy controls and periodontal pocket epithelia of patients. IL-10 was mainly expressed in the intercellular spaces of connective tissues. IL-1β displayed a reverse pattern of expression levels with reference to IL-10, and a negative correlation existed between LBP and the ratio of IL-1β/IL-10. rhLBP suppressed E. coli LPS-induced IL-1β expression by HGF. Conclusion: An appropriate interplay of LBP and cytokines may have a beneficial effect on innate host defence, thereby contributing to periodontal homeostasis. © 2009 John Wiley & Sons A/S.
Persistent Identifierhttp://hdl.handle.net/10722/66198
ISSN
2014 Impact Factor: 4.010
2014 SCImago Journal Rankings: 1.714
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
Funding Information:

This study was funded by the Hong Kong Research Grants Council (HKU 7518/05M to L.J. Jin).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorRen, Len_HK
dc.contributor.authorJiang, ZQen_HK
dc.contributor.authorFu, Yen_HK
dc.contributor.authorLeung, WKen_HK
dc.contributor.authorJin, Len_HK
dc.date.accessioned2010-09-06T05:44:23Z-
dc.date.available2010-09-06T05:44:23Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Clinical Periodontology, 2009, v. 36 n. 8, p. 619-626en_HK
dc.identifier.issn0303-6979en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66198-
dc.description.abstractAim: Periodontal pathogenesis is characterized by Gram-negative bacteria activation of series of pro- and anti-inflammatory cytokines from host cells through the pathway of lipopolysaccharide (LPS), LPS-binding protein (LBP) and CD14. The present study investigated the expression profiles of interleukin (IL)-1β and IL-10 in periodontal health and disease, and examined the effects of Escherichia coli LPS and LBP interaction on the expression of IL-1β and IL-10 by human gingival fibroblasts (HGF). Material and Methods: Gingival biopsies were collected from 44 subjects with chronic periodontitis and 15 periodontally healthy subjects. The expression of IL-1β and IL-10 was detected by immunohistochemistry. The mRNA expression of IL-1β and IL-10 in HGF was detected by RT-PCR with or without recombinant human LBP (rhLBP), while the peptides were analysed by an enzyme-linked immunosorbent assay. Results: IL-1β was detected in both oral sulcular epithelia of healthy controls and periodontal pocket epithelia of patients. IL-10 was mainly expressed in the intercellular spaces of connective tissues. IL-1β displayed a reverse pattern of expression levels with reference to IL-10, and a negative correlation existed between LBP and the ratio of IL-1β/IL-10. rhLBP suppressed E. coli LPS-induced IL-1β expression by HGF. Conclusion: An appropriate interplay of LBP and cytokines may have a beneficial effect on innate host defence, thereby contributing to periodontal homeostasis. © 2009 John Wiley & Sons A/S.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPEen_HK
dc.relation.ispartofJournal of Clinical Periodontologyen_HK
dc.subject.meshAcute-Phase Proteins - analysis - immunologyen_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAlveolar Bone Loss - immunology - pathologyen_HK
dc.subject.meshCarrier Proteins - analysis - immunologyen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshChronic Periodontitis - immunology - pathologyen_HK
dc.subject.meshConnective Tissue - immunology - pathologyen_HK
dc.subject.meshEpithelial Attachment - immunology - pathologyen_HK
dc.subject.meshEpithelium - immunology - pathologyen_HK
dc.subject.meshEscherichia coli - immunologyen_HK
dc.subject.meshExtracellular Space - immunologyen_HK
dc.subject.meshFibroblasts - immunologyen_HK
dc.subject.meshGingiva - cytology - immunologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunity, Innate - immunologyen_HK
dc.subject.meshInterleukin-10 - analysis - immunologyen_HK
dc.subject.meshInterleukin-1beta - analysis - immunologyen_HK
dc.subject.meshLipopolysaccharides - immunologyen_HK
dc.subject.meshMacrophages - immunology - pathologyen_HK
dc.subject.meshMembrane Glycoproteins - analysis - immunologyen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPeriodontal Attachment Loss - immunology - pathologyen_HK
dc.subject.meshPeriodontal Diseases - immunology - pathologyen_HK
dc.subject.meshPeriodontal Pocket - immunology - pathologyen_HK
dc.subject.meshPeriodontium - immunologyen_HK
dc.subject.meshRecombinant Proteinsen_HK
dc.subject.meshYoung Adulten_HK
dc.titleThe interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and diseaseen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0303-6979&volume=36&spage=619&epage=626&date=2009&atitle=The+interplay+of+lipopolysaccharide-binding+protein+and+cytokines+in+periodontal+health+and+diseaseen_HK
dc.identifier.emailLeung, WK:ewkleung@hkucc.hku.hken_HK
dc.identifier.emailJin, L:ljjin@hkucc.hku.hken_HK
dc.identifier.authorityLeung, WK=rp00019en_HK
dc.identifier.authorityJin, L=rp00028en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-051X.2009.01436.xen_HK
dc.identifier.pmid19558463-
dc.identifier.scopuseid_2-s2.0-68249097783en_HK
dc.identifier.hkuros158446en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-68249097783&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue8en_HK
dc.identifier.spage619en_HK
dc.identifier.epage626en_HK
dc.identifier.isiWOS:000267880900001-
dc.publisher.placeDenmarken_HK
dc.relation.projectNovel molecular mechanisms of innate host defense - implications in periodontal health and disease-
dc.identifier.scopusauthoridRen, L=35216223200en_HK
dc.identifier.scopusauthoridJiang, ZQ=35215029400en_HK
dc.identifier.scopusauthoridFu, Y=15077896300en_HK
dc.identifier.scopusauthoridLeung, WK=25224691800en_HK
dc.identifier.scopusauthoridJin, L=7403328850en_HK
dc.identifier.citeulike5406000-

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