Article: The interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and disease

File Download Links for fulltext
(May Require Subscription)
Supplementary
  • Basic View
  • Metadata View
  • XML View
TitleThe interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and disease
AuthorsRen, L2
Jiang, ZQ2
Fu, Y
Leung, WK1
Jin, L1
Issue Date2009
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
CitationJournal Of Clinical Periodontology, 2009, v. 36 n. 8, p. 619-626 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-051X.2009.01436.x
AbstractAim: Periodontal pathogenesis is characterized by Gram-negative bacteria activation of series of pro- and anti-inflammatory cytokines from host cells through the pathway of lipopolysaccharide (LPS), LPS-binding protein (LBP) and CD14. The present study investigated the expression profiles of interleukin (IL)-1β and IL-10 in periodontal health and disease, and examined the effects of Escherichia coli LPS and LBP interaction on the expression of IL-1β and IL-10 by human gingival fibroblasts (HGF). Material and Methods: Gingival biopsies were collected from 44 subjects with chronic periodontitis and 15 periodontally healthy subjects. The expression of IL-1β and IL-10 was detected by immunohistochemistry. The mRNA expression of IL-1β and IL-10 in HGF was detected by RT-PCR with or without recombinant human LBP (rhLBP), while the peptides were analysed by an enzyme-linked immunosorbent assay. Results: IL-1β was detected in both oral sulcular epithelia of healthy controls and periodontal pocket epithelia of patients. IL-10 was mainly expressed in the intercellular spaces of connective tissues. IL-1β displayed a reverse pattern of expression levels with reference to IL-10, and a negative correlation existed between LBP and the ratio of IL-1β/IL-10. rhLBP suppressed E. coli LPS-induced IL-1β expression by HGF. Conclusion: An appropriate interplay of LBP and cytokines may have a beneficial effect on innate host defence, thereby contributing to periodontal homeostasis. © 2009 John Wiley & Sons A/S.
ISSN0303-6979
2011 Impact Factor: 2.996
2011 SCImago Journal Rankings: 0.160
DOIhttp://dx.doi.org/10.1111/j.1600-051X.2009.01436.x
ISI Accession Number IDWOS:000267880900001
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
Funding Information:

This study was funded by the Hong Kong Research Grants Council (HKU 7518/05M to L.J. Jin).

ReferencesReferences in Scopus
GrantsNovel molecular mechanisms of innate host defense - implications in periodontal health and disease
Novel molecular mechanisms of innate host defense - implications in periodontal health and disease
DC Field
Value
dc.contributor.authorRen, L
dc.contributor.authorJiang, ZQ
dc.contributor.authorFu, Y
dc.contributor.authorLeung, WK
dc.contributor.authorJin, L
dc.date.accessioned2010-09-06T05:44:23Z
dc.date.available2010-09-06T05:44:23Z
dc.date.issued2009
dc.description.abstractAim: Periodontal pathogenesis is characterized by Gram-negative bacteria activation of series of pro- and anti-inflammatory cytokines from host cells through the pathway of lipopolysaccharide (LPS), LPS-binding protein (LBP) and CD14. The present study investigated the expression profiles of interleukin (IL)-1β and IL-10 in periodontal health and disease, and examined the effects of Escherichia coli LPS and LBP interaction on the expression of IL-1β and IL-10 by human gingival fibroblasts (HGF). Material and Methods: Gingival biopsies were collected from 44 subjects with chronic periodontitis and 15 periodontally healthy subjects. The expression of IL-1β and IL-10 was detected by immunohistochemistry. The mRNA expression of IL-1β and IL-10 in HGF was detected by RT-PCR with or without recombinant human LBP (rhLBP), while the peptides were analysed by an enzyme-linked immunosorbent assay. Results: IL-1β was detected in both oral sulcular epithelia of healthy controls and periodontal pocket epithelia of patients. IL-10 was mainly expressed in the intercellular spaces of connective tissues. IL-1β displayed a reverse pattern of expression levels with reference to IL-10, and a negative correlation existed between LBP and the ratio of IL-1β/IL-10. rhLBP suppressed E. coli LPS-induced IL-1β expression by HGF. Conclusion: An appropriate interplay of LBP and cytokines may have a beneficial effect on innate host defence, thereby contributing to periodontal homeostasis. © 2009 John Wiley & Sons A/S.
dc.description.grantNovel molecular mechanisms of innate host defense - implications in periodontal health and disease
dc.description.grantNovel molecular mechanisms of innate host defense - implications in periodontal health and disease
dc.description.grantcode28079
dc.description.grantcode29685
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Clinical Periodontology, 2009, v. 36 n. 8, p. 619-626 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-051X.2009.01436.x
dc.identifier.citeulike5406000
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-051X.2009.01436.x
dc.identifier.epage626
dc.identifier.hkuros158446
dc.identifier.isiWOS:000267880900001
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
Funding Information:

This study was funded by the Hong Kong Research Grants Council (HKU 7518/05M to L.J. Jin).

dc.identifier.issn0303-6979
2011 Impact Factor: 2.996
2011 SCImago Journal Rankings: 0.160
dc.identifier.issue8
dc.identifier.openurl
dc.identifier.pmid19558463
dc.identifier.scopuseid_2-s2.0-68249097783
dc.identifier.spage619
dc.identifier.urihttp://hdl.handle.net/10722/66198
dc.identifier.volume36
dc.languageeng
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
dc.publisher.placeDenmark
dc.relation.ispartofJournal of Clinical Periodontology
dc.relation.referencesReferences in Scopus
dc.subject.meshAcute-Phase Proteins - analysis - immunology
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshAlveolar Bone Loss - immunology - pathology
dc.subject.meshCarrier Proteins - analysis - immunology
dc.subject.meshCells, Cultured
dc.subject.meshChronic Periodontitis - immunology - pathology
dc.subject.meshConnective Tissue - immunology - pathology
dc.subject.meshEpithelial Attachment - immunology - pathology
dc.subject.meshEpithelium - immunology - pathology
dc.subject.meshEscherichia coli - immunology
dc.subject.meshExtracellular Space - immunology
dc.subject.meshFibroblasts - immunology
dc.subject.meshGingiva - cytology - immunology
dc.subject.meshHumans
dc.subject.meshImmunity, Innate - immunology
dc.subject.meshInterleukin-10 - analysis - immunology
dc.subject.meshInterleukin-1beta - analysis - immunology
dc.subject.meshLipopolysaccharides - immunology
dc.subject.meshMacrophages - immunology - pathology
dc.subject.meshMembrane Glycoproteins - analysis - immunology
dc.subject.meshMiddle Aged
dc.subject.meshPeriodontal Attachment Loss - immunology - pathology
dc.subject.meshPeriodontal Diseases - immunology - pathology
dc.subject.meshPeriodontal Pocket - immunology - pathology
dc.subject.meshPeriodontium - immunology
dc.subject.meshRecombinant Proteins
dc.subject.meshYoung Adult
dc.titleThe interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and disease
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Sun Yat-Sen University