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Article: The interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and disease
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TitleThe interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and disease
 
AuthorsRen, L2
Jiang, ZQ2
Fu, Y
Leung, WK1
Jin, L1
 
Issue Date2009
 
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
 
CitationJournal Of Clinical Periodontology, 2009, v. 36 n. 8, p. 619-626 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-051X.2009.01436.x
 
AbstractAim: Periodontal pathogenesis is characterized by Gram-negative bacteria activation of series of pro- and anti-inflammatory cytokines from host cells through the pathway of lipopolysaccharide (LPS), LPS-binding protein (LBP) and CD14. The present study investigated the expression profiles of interleukin (IL)-1β and IL-10 in periodontal health and disease, and examined the effects of Escherichia coli LPS and LBP interaction on the expression of IL-1β and IL-10 by human gingival fibroblasts (HGF). Material and Methods: Gingival biopsies were collected from 44 subjects with chronic periodontitis and 15 periodontally healthy subjects. The expression of IL-1β and IL-10 was detected by immunohistochemistry. The mRNA expression of IL-1β and IL-10 in HGF was detected by RT-PCR with or without recombinant human LBP (rhLBP), while the peptides were analysed by an enzyme-linked immunosorbent assay. Results: IL-1β was detected in both oral sulcular epithelia of healthy controls and periodontal pocket epithelia of patients. IL-10 was mainly expressed in the intercellular spaces of connective tissues. IL-1β displayed a reverse pattern of expression levels with reference to IL-10, and a negative correlation existed between LBP and the ratio of IL-1β/IL-10. rhLBP suppressed E. coli LPS-induced IL-1β expression by HGF. Conclusion: An appropriate interplay of LBP and cytokines may have a beneficial effect on innate host defence, thereby contributing to periodontal homeostasis. © 2009 John Wiley & Sons A/S.
 
ISSN0303-6979
2012 Impact Factor: 3.688
2012 SCImago Journal Rankings: 1.298
 
DOIhttp://dx.doi.org/10.1111/j.1600-051X.2009.01436.x
 
ISI Accession Number IDWOS:000267880900001
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
Funding Information:

This study was funded by the Hong Kong Research Grants Council (HKU 7518/05M to L.J. Jin).

 
ReferencesReferences in Scopus
 
GrantsNovel molecular mechanisms of innate host defense - implications in periodontal health and disease
 
DC FieldValue
dc.contributor.authorRen, L
 
dc.contributor.authorJiang, ZQ
 
dc.contributor.authorFu, Y
 
dc.contributor.authorLeung, WK
 
dc.contributor.authorJin, L
 
dc.date.accessioned2010-09-06T05:44:23Z
 
dc.date.available2010-09-06T05:44:23Z
 
dc.date.issued2009
 
dc.description.abstractAim: Periodontal pathogenesis is characterized by Gram-negative bacteria activation of series of pro- and anti-inflammatory cytokines from host cells through the pathway of lipopolysaccharide (LPS), LPS-binding protein (LBP) and CD14. The present study investigated the expression profiles of interleukin (IL)-1β and IL-10 in periodontal health and disease, and examined the effects of Escherichia coli LPS and LBP interaction on the expression of IL-1β and IL-10 by human gingival fibroblasts (HGF). Material and Methods: Gingival biopsies were collected from 44 subjects with chronic periodontitis and 15 periodontally healthy subjects. The expression of IL-1β and IL-10 was detected by immunohistochemistry. The mRNA expression of IL-1β and IL-10 in HGF was detected by RT-PCR with or without recombinant human LBP (rhLBP), while the peptides were analysed by an enzyme-linked immunosorbent assay. Results: IL-1β was detected in both oral sulcular epithelia of healthy controls and periodontal pocket epithelia of patients. IL-10 was mainly expressed in the intercellular spaces of connective tissues. IL-1β displayed a reverse pattern of expression levels with reference to IL-10, and a negative correlation existed between LBP and the ratio of IL-1β/IL-10. rhLBP suppressed E. coli LPS-induced IL-1β expression by HGF. Conclusion: An appropriate interplay of LBP and cytokines may have a beneficial effect on innate host defence, thereby contributing to periodontal homeostasis. © 2009 John Wiley & Sons A/S.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Clinical Periodontology, 2009, v. 36 n. 8, p. 619-626 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-051X.2009.01436.x
 
dc.identifier.citeulike5406000
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-051X.2009.01436.x
 
dc.identifier.epage626
 
dc.identifier.hkuros158446
 
dc.identifier.isiWOS:000267880900001
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7518/05M
Funding Information:

This study was funded by the Hong Kong Research Grants Council (HKU 7518/05M to L.J. Jin).

 
dc.identifier.issn0303-6979
2012 Impact Factor: 3.688
2012 SCImago Journal Rankings: 1.298
 
dc.identifier.issue8
 
dc.identifier.openurl
 
dc.identifier.pmid19558463
 
dc.identifier.scopuseid_2-s2.0-68249097783
 
dc.identifier.spage619
 
dc.identifier.urihttp://hdl.handle.net/10722/66198
 
dc.identifier.volume36
 
dc.languageeng
 
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
 
dc.publisher.placeDenmark
 
dc.relation.ispartofJournal of Clinical Periodontology
 
dc.relation.projectNovel molecular mechanisms of innate host defense - implications in periodontal health and disease
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAcute-Phase Proteins - analysis - immunology
 
dc.subject.meshAdolescent
 
dc.subject.meshAdult
 
dc.subject.meshAged
 
dc.subject.meshAlveolar Bone Loss - immunology - pathology
 
dc.subject.meshCarrier Proteins - analysis - immunology
 
dc.subject.meshCells, Cultured
 
dc.subject.meshChronic Periodontitis - immunology - pathology
 
dc.subject.meshConnective Tissue - immunology - pathology
 
dc.subject.meshEpithelial Attachment - immunology - pathology
 
dc.subject.meshEpithelium - immunology - pathology
 
dc.subject.meshEscherichia coli - immunology
 
dc.subject.meshExtracellular Space - immunology
 
dc.subject.meshFibroblasts - immunology
 
dc.subject.meshGingiva - cytology - immunology
 
dc.subject.meshHumans
 
dc.subject.meshImmunity, Innate - immunology
 
dc.subject.meshInterleukin-10 - analysis - immunology
 
dc.subject.meshInterleukin-1beta - analysis - immunology
 
dc.subject.meshLipopolysaccharides - immunology
 
dc.subject.meshMacrophages - immunology - pathology
 
dc.subject.meshMembrane Glycoproteins - analysis - immunology
 
dc.subject.meshMiddle Aged
 
dc.subject.meshPeriodontal Attachment Loss - immunology - pathology
 
dc.subject.meshPeriodontal Diseases - immunology - pathology
 
dc.subject.meshPeriodontal Pocket - immunology - pathology
 
dc.subject.meshPeriodontium - immunology
 
dc.subject.meshRecombinant Proteins
 
dc.subject.meshYoung Adult
 
dc.titleThe interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and disease
 
dc.typeArticle
 
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<item><contributor.author>Ren, L</contributor.author>
<contributor.author>Jiang, ZQ</contributor.author>
<contributor.author>Fu, Y</contributor.author>
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<description.abstract>Aim: Periodontal pathogenesis is characterized by Gram-negative bacteria activation of series of pro- and anti-inflammatory cytokines from host cells through the pathway of lipopolysaccharide (LPS), LPS-binding protein (LBP) and CD14. The present study investigated the expression profiles of interleukin (IL)-1&#946; and IL-10 in periodontal health and disease, and examined the effects of Escherichia coli LPS and LBP interaction on the expression of IL-1&#946; and IL-10 by human gingival fibroblasts (HGF). Material and Methods: Gingival biopsies were collected from 44 subjects with chronic periodontitis and 15 periodontally healthy subjects. The expression of IL-1&#946; and IL-10 was detected by immunohistochemistry. The mRNA expression of IL-1&#946; and IL-10 in HGF was detected by RT-PCR with or without recombinant human LBP (rhLBP), while the peptides were analysed by an enzyme-linked immunosorbent assay. Results: IL-1&#946; was detected in both oral sulcular epithelia of healthy controls and periodontal pocket epithelia of patients. IL-10 was mainly expressed in the intercellular spaces of connective tissues. IL-1&#946; displayed a reverse pattern of expression levels with reference to IL-10, and a negative correlation existed between LBP and the ratio of IL-1&#946;/IL-10. rhLBP suppressed E. coli LPS-induced IL-1&#946; expression by HGF. Conclusion: An appropriate interplay of LBP and cytokines may have a beneficial effect on innate host defence, thereby contributing to periodontal homeostasis. &#169; 2009 John Wiley &amp; Sons A/S.</description.abstract>
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Author Affiliations
  1. The University of Hong Kong
  2. Sun Yat-Sen University