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Article: Heterogeneity in antifungal susceptibility of clones of Candida albicans isolated on single and sequential visits from a HIV-infected southern Chinese cohort

TitleHeterogeneity in antifungal susceptibility of clones of Candida albicans isolated on single and sequential visits from a HIV-infected southern Chinese cohort
Authors
Issue Date2001
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPM
Citation
Journal Of Oral Pathology And Medicine, 2001, v. 30 n. 6, p. 336-346 How to Cite?
AbstractThe increased frequency and severity of candidal infections in human immunodeficiency virus (HIV)-infected individuals has prompted the wide use of antifungals, such as amphotericin B, ketoconazole, and fluconazole, resulting in the emergence of drug-resistant strains of Candida albicans. To study this phenomenon in an ethnic Chinese cohort, we isolated multiple colonies of Candida from the oral cavities of 16 HIV-infected patients on single and subsequent sequential visits over a period of 12 months. Ten of the 16 patients had sporadic episodes of oropharyngeal candidiasis (Group A), while the remainder were asymptomatic with respect to this condition (Group B). Oral rinses were collected and immediately processed in the laboratory for the isolation of C. albicans in a standard manner. A total of 433 C. albicans isolates were tested for their susceptibility to amphotericin B, ketoconazole and fluconazole by an agar diffusion method using the commercially available E-test. All tested isolates demonstrated variable susceptibility to amphotericin B, ketoconazole and fluconazole. The minimum inhibitory concentration (MIC) of the isolates for amphotericin B, ketoconazole and fluconazole ranged from <0.002-1.5 μg/ml, <0.002-4.0 μg/ml and <0.016-32 μg/ml, respectively. Sequential isolates of a few patients demonstrated variable susceptibility to all the antifungals, and no discernible MIC pattern emerged either in group A or B over time. Interestingly, significant variation in antifungal susceptibility was also noted in isolates obtained from the same patient on a single visit. Sequential yeast isolates in 9 of 16 patients (56%) demonstrated significant differences in MIC within and between visits for both amphotericin B and ketoconazole, while a lower percentage - 44% (7/16) - exhibited this trait for fluconazole. Our study demonstrates the diversity in antifungal susceptibility in either commensal or "infective" oral strains of C. albicans in HIV disease, and shows the need for vigilance for the emergence of resistant strains, and for frequent antifungal susceptibility studies.
Persistent Identifierhttp://hdl.handle.net/10722/66130
ISSN
2015 Impact Factor: 1.859
2015 SCImago Journal Rankings: 0.731
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.contributor.authorTsang, PCen_HK
dc.contributor.authorWong, KHen_HK
dc.contributor.authorYeung, KWSen_HK
dc.date.accessioned2010-09-06T05:43:49Z-
dc.date.available2010-09-06T05:43:49Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Oral Pathology And Medicine, 2001, v. 30 n. 6, p. 336-346en_HK
dc.identifier.issn0904-2512en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66130-
dc.description.abstractThe increased frequency and severity of candidal infections in human immunodeficiency virus (HIV)-infected individuals has prompted the wide use of antifungals, such as amphotericin B, ketoconazole, and fluconazole, resulting in the emergence of drug-resistant strains of Candida albicans. To study this phenomenon in an ethnic Chinese cohort, we isolated multiple colonies of Candida from the oral cavities of 16 HIV-infected patients on single and subsequent sequential visits over a period of 12 months. Ten of the 16 patients had sporadic episodes of oropharyngeal candidiasis (Group A), while the remainder were asymptomatic with respect to this condition (Group B). Oral rinses were collected and immediately processed in the laboratory for the isolation of C. albicans in a standard manner. A total of 433 C. albicans isolates were tested for their susceptibility to amphotericin B, ketoconazole and fluconazole by an agar diffusion method using the commercially available E-test. All tested isolates demonstrated variable susceptibility to amphotericin B, ketoconazole and fluconazole. The minimum inhibitory concentration (MIC) of the isolates for amphotericin B, ketoconazole and fluconazole ranged from <0.002-1.5 μg/ml, <0.002-4.0 μg/ml and <0.016-32 μg/ml, respectively. Sequential isolates of a few patients demonstrated variable susceptibility to all the antifungals, and no discernible MIC pattern emerged either in group A or B over time. Interestingly, significant variation in antifungal susceptibility was also noted in isolates obtained from the same patient on a single visit. Sequential yeast isolates in 9 of 16 patients (56%) demonstrated significant differences in MIC within and between visits for both amphotericin B and ketoconazole, while a lower percentage - 44% (7/16) - exhibited this trait for fluconazole. Our study demonstrates the diversity in antifungal susceptibility in either commensal or "infective" oral strains of C. albicans in HIV disease, and shows the need for vigilance for the emergence of resistant strains, and for frequent antifungal susceptibility studies.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/JOPMen_HK
dc.relation.ispartofJournal of Oral Pathology and Medicineen_HK
dc.subject.meshAIDS-Related Opportunistic Infections - drug therapy - microbiologyen_HK
dc.subject.meshAdulten_HK
dc.subject.meshAmphotericin B - therapeutic useen_HK
dc.subject.meshAnalysis of Varianceen_HK
dc.subject.meshAntifungal Agents - therapeutic useen_HK
dc.subject.meshCandida albicans - classification - drug effectsen_HK
dc.subject.meshCandidiasis, Oral - drug therapy - microbiologyen_HK
dc.subject.meshChinaen_HK
dc.subject.meshCohort Studiesen_HK
dc.subject.meshDrug Resistance, Microbial - geneticsen_HK
dc.subject.meshEthnic Groupsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFluconazole - therapeutic useen_HK
dc.subject.meshFollow-Up Studiesen_HK
dc.subject.meshHIV Infections - complicationsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKetoconazole - therapeutic useen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshOropharynx - microbiologyen_HK
dc.subject.meshPharyngeal Diseases - microbiologyen_HK
dc.subject.meshRecurrenceen_HK
dc.titleHeterogeneity in antifungal susceptibility of clones of Candida albicans isolated on single and sequential visits from a HIV-infected southern Chinese cohorten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0904-2512&volume=30&spage=336&epage=46&date=2000&atitle=Heterogeneity+in+antifungal+susceptibility+of+clones+of+Candida+albicans+isolated+on+single+and+sequential+visits+from+a+HIV-infected+southern+Chinese+cohorten_HK
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.emailTsang, PC:csptsang@hkucc.hku.hken_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.identifier.authorityTsang, PC=rp00026en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1034/j.1600-0714.2001.300603.xen_HK
dc.identifier.pmid11459319-
dc.identifier.scopuseid_2-s2.0-0034940296en_HK
dc.identifier.hkuros62641en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034940296&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue6en_HK
dc.identifier.spage336en_HK
dc.identifier.epage346en_HK
dc.identifier.isiWOS:000169681900003-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.scopusauthoridTsang, PC=7202936002en_HK
dc.identifier.scopusauthoridWong, KH=7404758411en_HK
dc.identifier.scopusauthoridYeung, KWS=13304249300en_HK

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