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Article: Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms

TitleArchitectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms
Authors
Issue Date2009
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbio
Citation
Archives Of Oral Biology, 2009, v. 54 n. 11, p. 1052-1060 How to Cite?
AbstractThe human fungal pathogen Candida is able to form biofilms in almost all the medical devices in current use. Indeed, biofilm formation is a major virulence attribute of microorganisms and account for a majority of human infections. Therefore, understanding processes appertaining to biofilm development is an important prerequisite for devising new strategies to prevent or eradicate biofilm-related infections. In the present study we used an array of both conventional and novel analytical tools to obtain a comprehensive view of Candida biofilm development. Enumeration of colony forming units, colorimetric (XTT) assay, Scanning Electron Microscopy (SEM) and novel Confocal Laser Scanning Microscopy (CLSM) coupled with COMSTAT software analyses were utilised to evaluate growth kinetics; architecture and viability of biofilms of a reference (ATCC) and a clinical strain each of two Candida species, C. albicans and C. glabrata. Biofilm growth kinetics on a polystyrene substrate was evaluated from the initial adhesion step (1.5 h) up to 72 h. These analyses revealed substantial inter- and intra-species differences in temporal organisation of Candida biofilm architecture, spatiality and cellular viability, while reaching maturity within a period of 48 h, on a polystyrene substrate. There were substantial differences in the growth kinetics upon methodology, although general trend seemed to be the same. Detailed architectural analysis provided by COMSTAT software corroborated the SEM and CSLM views. These analyses may provide a strong foundation for down stream molecular work of fungal biofilms. © 2009 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/66101
ISSN
2015 Impact Factor: 1.733
2015 SCImago Journal Rankings: 0.713
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong ResearchHKU 7624/06M
Funding Information:

Funding: This work was supported by the Hong Kong Research Grants Council, RGC No. HKU 7624/06M.

References
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DC FieldValueLanguage
dc.contributor.authorSeneviratne, CJen_HK
dc.contributor.authorSilva, WJen_HK
dc.contributor.authorJin, LJen_HK
dc.contributor.authorSamaranayake, YHen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:43:35Z-
dc.date.available2010-09-06T05:43:35Z-
dc.date.issued2009en_HK
dc.identifier.citationArchives Of Oral Biology, 2009, v. 54 n. 11, p. 1052-1060en_HK
dc.identifier.issn0003-9969en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66101-
dc.description.abstractThe human fungal pathogen Candida is able to form biofilms in almost all the medical devices in current use. Indeed, biofilm formation is a major virulence attribute of microorganisms and account for a majority of human infections. Therefore, understanding processes appertaining to biofilm development is an important prerequisite for devising new strategies to prevent or eradicate biofilm-related infections. In the present study we used an array of both conventional and novel analytical tools to obtain a comprehensive view of Candida biofilm development. Enumeration of colony forming units, colorimetric (XTT) assay, Scanning Electron Microscopy (SEM) and novel Confocal Laser Scanning Microscopy (CLSM) coupled with COMSTAT software analyses were utilised to evaluate growth kinetics; architecture and viability of biofilms of a reference (ATCC) and a clinical strain each of two Candida species, C. albicans and C. glabrata. Biofilm growth kinetics on a polystyrene substrate was evaluated from the initial adhesion step (1.5 h) up to 72 h. These analyses revealed substantial inter- and intra-species differences in temporal organisation of Candida biofilm architecture, spatiality and cellular viability, while reaching maturity within a period of 48 h, on a polystyrene substrate. There were substantial differences in the growth kinetics upon methodology, although general trend seemed to be the same. Detailed architectural analysis provided by COMSTAT software corroborated the SEM and CSLM views. These analyses may provide a strong foundation for down stream molecular work of fungal biofilms. © 2009 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/archoralbioen_HK
dc.relation.ispartofArchives of Oral Biologyen_HK
dc.subject.meshBiofilms - growth & developmenten_HK
dc.subject.meshBiomassen_HK
dc.subject.meshCandida albicans - growth & developmenten_HK
dc.subject.meshCandida glabrata - growth & developmenten_HK
dc.subject.meshCell Adhesionen_HK
dc.subject.meshColony Count, Microbialen_HK
dc.subject.meshMicrobial Viabilityen_HK
dc.subject.meshMicroscopy, Confocalen_HK
dc.subject.meshMicroscopy, Electron, Scanningen_HK
dc.subject.meshPolystyrenesen_HK
dc.subject.meshSoftwareen_HK
dc.titleArchitectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilmsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0003-9969&volume=54&spage=1052&epage=1060&date=2009&atitle=Architectural+analysis,+viability+assessment+and+growth+kinetics+of+Candida+albicans+and+Candida+glabrata+biofilmsen_HK
dc.identifier.emailSeneviratne, CJ:jaya@hku.hken_HK
dc.identifier.emailJin, LJ:ljjin@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, YH:hema@hkucc.hku.hken_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySeneviratne, CJ=rp01372en_HK
dc.identifier.authorityJin, LJ=rp00028en_HK
dc.identifier.authoritySamaranayake, YH=rp00025en_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.archoralbio.2009.08.002en_HK
dc.identifier.pmid19712926-
dc.identifier.scopuseid_2-s2.0-70349779177en_HK
dc.identifier.hkuros164360en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70349779177&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume54en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1052en_HK
dc.identifier.epage1060en_HK
dc.identifier.isiWOS:000271439500011-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectCandida biofilms: molecular mechanisms and clinical implications-
dc.identifier.scopusauthoridSeneviratne, CJ=6701897753en_HK
dc.identifier.scopusauthoridSilva, WJ=7006750532en_HK
dc.identifier.scopusauthoridJin, LJ=7403328850en_HK
dc.identifier.scopusauthoridSamaranayake, YH=6602677237en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK
dc.identifier.citeulike5670987-

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