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Article: The impact of polyene, azole, and DNA analogue antimycotics on the cell surface hydrophobicity of Candida albicans and Candida tropicalis in HIV infection

TitleThe impact of polyene, azole, and DNA analogue antimycotics on the cell surface hydrophobicity of Candida albicans and Candida tropicalis in HIV infection
Authors
Issue Date2002
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0301-486X
Citation
Mycopathologia, 2002, v. 153 n. 4, p. 179-185 How to Cite?
AbstractOral candidiasis is the most common opportunistic infection in individuals infected with the human immunodeficiency virus. Though Candida albicans is the major aetiological agent, non-albicans species such Candida tropicalis are now emerging as important agents of such infection. The Candida cell surface hydrophobicity (CSH) is considered a critical factor contributing to its colonization potential and virulence. It is also known that brief exposure to sub-cidal concentrations of antifungal agents is a likely scenario in the oral environment where the administered drugs are diluted continuously due to the flushing action of saliva. Hence the objective of the present study was to compare the CSH of 10 isolates each of C. albicans and C. tropicalis from HIV-infected individuals following brief exposure (1hour) of isolates to sub-therapeutic concentrations of nystatin, amphotericin B, ketoconazole, fluconazole and 5-flurocytosine. The CSH was assessed by a previously described biphasic aqueous-hydrocarbon assay. The mean percentage reduction of CSH of C. albicans following brief exposure to nystatin, amphotericin B, ketoconazole, fluconazole and 5-flurocytosine was 27.33 (p < 0.001), 21.34 (p < 0.05), 11.74 (p > 0.05), 18.4 (p > 0.05) and 14.64 (p > 0.05) respectively. The mean percentage reduction of CSH of C. tropicalis following brief exposure to nystatin, amphotericin B, ketoconazole, fluconazole and 5-flurocytosine was 33.81 (p < 0.01), 28.88 (p < 0.01), 12.6 (p > 0.05), 21.53 (p > 0.05) and 17.68 (p > 0.05) respectively. A significant interspecies variation in CSH was observed for nystatin and amphoterecin B. Overall the results reveal that the CSH of C. albicans is affected to a significantly lesser degree compared with C. tropicalis when exposed to the antifungals. These data further illustrate another mode of action of antifungals on Candida leading to a reduction in the CSH and thereby the yeast adherence to host tissues.
Persistent Identifierhttp://hdl.handle.net/10722/66074
ISSN
2015 Impact Factor: 1.671
2015 SCImago Journal Rankings: 0.720
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAnil, Sen_HK
dc.contributor.authorEllepola, ANBen_HK
dc.contributor.authorSamaranayake, LPen_HK
dc.date.accessioned2010-09-06T05:43:22Z-
dc.date.available2010-09-06T05:43:22Z-
dc.date.issued2002en_HK
dc.identifier.citationMycopathologia, 2002, v. 153 n. 4, p. 179-185en_HK
dc.identifier.issn0301-486Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/66074-
dc.description.abstractOral candidiasis is the most common opportunistic infection in individuals infected with the human immunodeficiency virus. Though Candida albicans is the major aetiological agent, non-albicans species such Candida tropicalis are now emerging as important agents of such infection. The Candida cell surface hydrophobicity (CSH) is considered a critical factor contributing to its colonization potential and virulence. It is also known that brief exposure to sub-cidal concentrations of antifungal agents is a likely scenario in the oral environment where the administered drugs are diluted continuously due to the flushing action of saliva. Hence the objective of the present study was to compare the CSH of 10 isolates each of C. albicans and C. tropicalis from HIV-infected individuals following brief exposure (1hour) of isolates to sub-therapeutic concentrations of nystatin, amphotericin B, ketoconazole, fluconazole and 5-flurocytosine. The CSH was assessed by a previously described biphasic aqueous-hydrocarbon assay. The mean percentage reduction of CSH of C. albicans following brief exposure to nystatin, amphotericin B, ketoconazole, fluconazole and 5-flurocytosine was 27.33 (p < 0.001), 21.34 (p < 0.05), 11.74 (p > 0.05), 18.4 (p > 0.05) and 14.64 (p > 0.05) respectively. The mean percentage reduction of CSH of C. tropicalis following brief exposure to nystatin, amphotericin B, ketoconazole, fluconazole and 5-flurocytosine was 33.81 (p < 0.01), 28.88 (p < 0.01), 12.6 (p > 0.05), 21.53 (p > 0.05) and 17.68 (p > 0.05) respectively. A significant interspecies variation in CSH was observed for nystatin and amphoterecin B. Overall the results reveal that the CSH of C. albicans is affected to a significantly lesser degree compared with C. tropicalis when exposed to the antifungals. These data further illustrate another mode of action of antifungals on Candida leading to a reduction in the CSH and thereby the yeast adherence to host tissues.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0301-486Xen_HK
dc.relation.ispartofMycopathologiaen_HK
dc.subject.meshAIDS-Related Opportunistic Infections - microbiologyen_HK
dc.subject.meshAntifungal Agents - pharmacologyen_HK
dc.subject.meshAzoles - pharmacologyen_HK
dc.subject.meshCandida albicans - drug effectsen_HK
dc.subject.meshCandida tropicalis - drug effectsen_HK
dc.subject.meshCandidiasis, Oral - microbiologyen_HK
dc.subject.meshCell Membrane - drug effectsen_HK
dc.subject.meshDNA - chemistryen_HK
dc.subject.meshHIV Infections - complicationsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHydrophobic and Hydrophilic Interactionsen_HK
dc.subject.meshMicrobial Sensitivity Testsen_HK
dc.subject.meshPolyenes - pharmacologyen_HK
dc.titleThe impact of polyene, azole, and DNA analogue antimycotics on the cell surface hydrophobicity of Candida albicans and Candida tropicalis in HIV infectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0301-486X&volume=153&spage=179&epage=185&date=2002&atitle=The+impact+of+polyene,+azole,+and+DNA+analogue+antimycotics+on+the+cell+surface+hydrophobicity+of+Candida+albicans+and+Candida+tropicalis+in+HIV+infectionen_HK
dc.identifier.emailSamaranayake, LP:lakshman@hku.hken_HK
dc.identifier.authoritySamaranayake, LP=rp00023en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1023/A:1014932302518en_HK
dc.identifier.pmid12014477-
dc.identifier.scopuseid_2-s2.0-0036103049en_HK
dc.identifier.hkuros65796en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036103049&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume153en_HK
dc.identifier.issue4en_HK
dc.identifier.spage179en_HK
dc.identifier.epage185en_HK
dc.identifier.isiWOS:000175273300002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridAnil, S=7006640037en_HK
dc.identifier.scopusauthoridEllepola, ANB=6604060863en_HK
dc.identifier.scopusauthoridSamaranayake, LP=7102761002en_HK

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