Article: Pro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: A proof-of-concept study

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TitlePro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: A proof-of-concept study
AuthorsSalvi, GE2
Franco, LM3
Braun, TM3
Lee, A3
Rutger Persson, G2
Lang, NP1 2
Giannobile, WV3
Issue Date2010
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
CitationJournal Of Clinical Periodontology, 2010, v. 37 n. 1, p. 9-16 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-051X.2009.01500.x
AbstractAbstract Aim: To compare gingival crevicular fluid (GCF) biomarker levels and microbial distribution in plaque biofilm (SP) samples for subjects with type 1 diabetes (T1DM) versus healthy subjects without diabetes during experimental gingivitis (EG). Materials and Methods: A total of nine T1DM patients and nine healthy controls of age and gender similar to the T1DM patients were monitored for 35 days during EG. Hygiene practices were stopped for 3 weeks, and GCF, SP, plaque index (PI) and gingival index were determined. IL-1β, IL-8, MMP-8 and MMP-9 were quantified by enzyme-linked immunosorbent assay, and SP samples were assessed by DNA-DNA hybridization for a panel of 40 subgingival microbial species. Results: IL-1β levels in T1DM patients were elevated compared with healthy individuals, and showed differences between groups at 7-21 days while healthy patients showed IL-1β increases from baseline to 14-21 days (p<0.05). Differences were observed in MMP-9 levels between patients with and without T1DM at 7-14 days (p<0.05). Orange complex species and PI measurements displayed a superior correlation with biomarker levels when compared with other complexes or clinical measurements during EG. Conclusions: The mean GCF biomarker levels for IL-1β and MMP-8 were most significantly elevated in T1DM subjects compared with healthy individuals during EG, not resulting from differences in the mean PI or microbial composition. © 2009 John Wiley & Sons A/S.
ISSN0303-6979
2011 Impact Factor: 2.996
2011 SCImago Journal Rankings: 0.160
DOIhttp://dx.doi.org/10.1111/j.1600-051X.2009.01500.x
ISI Accession Number IDWOS:000272771700002
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorSalvi, GE
dc.contributor.authorFranco, LM
dc.contributor.authorBraun, TM
dc.contributor.authorLee, A
dc.contributor.authorRutger Persson, G
dc.contributor.authorLang, NP
dc.contributor.authorGiannobile, WV
dc.date.accessioned2010-09-06T05:43:12Z
dc.date.available2010-09-06T05:43:12Z
dc.date.issued2010
dc.description.abstractAbstract Aim: To compare gingival crevicular fluid (GCF) biomarker levels and microbial distribution in plaque biofilm (SP) samples for subjects with type 1 diabetes (T1DM) versus healthy subjects without diabetes during experimental gingivitis (EG). Materials and Methods: A total of nine T1DM patients and nine healthy controls of age and gender similar to the T1DM patients were monitored for 35 days during EG. Hygiene practices were stopped for 3 weeks, and GCF, SP, plaque index (PI) and gingival index were determined. IL-1β, IL-8, MMP-8 and MMP-9 were quantified by enzyme-linked immunosorbent assay, and SP samples were assessed by DNA-DNA hybridization for a panel of 40 subgingival microbial species. Results: IL-1β levels in T1DM patients were elevated compared with healthy individuals, and showed differences between groups at 7-21 days while healthy patients showed IL-1β increases from baseline to 14-21 days (p<0.05). Differences were observed in MMP-9 levels between patients with and without T1DM at 7-14 days (p<0.05). Orange complex species and PI measurements displayed a superior correlation with biomarker levels when compared with other complexes or clinical measurements during EG. Conclusions: The mean GCF biomarker levels for IL-1β and MMP-8 were most significantly elevated in T1DM subjects compared with healthy individuals during EG, not resulting from differences in the mean PI or microbial composition. © 2009 John Wiley & Sons A/S.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationJournal Of Clinical Periodontology, 2010, v. 37 n. 1, p. 9-16 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-051X.2009.01500.x
dc.identifier.citeulike6466146
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-051X.2009.01500.x
dc.identifier.epage16
dc.identifier.hkuros169087
dc.identifier.isiWOS:000272771700002
dc.identifier.issn0303-6979
2011 Impact Factor: 2.996
2011 SCImago Journal Rankings: 0.160
dc.identifier.issue1
dc.identifier.openurl
dc.identifier.pmid19958441
dc.identifier.scopuseid_2-s2.0-72549107943
dc.identifier.spage9
dc.identifier.urihttp://hdl.handle.net/10722/66053
dc.identifier.volume37
dc.languageeng
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
dc.publisher.placeDenmark
dc.relation.ispartofJournal of Clinical Periodontology
dc.relation.referencesReferences in Scopus
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshBacteria - classification
dc.subject.meshBiofilms
dc.subject.meshBiological Markers - analysis
dc.subject.meshCase-Control Studies
dc.subject.meshCohort Studies
dc.subject.meshDental Plaque - microbiology
dc.subject.meshDental Plaque Index
dc.subject.meshDiabetes Mellitus, Type 1 - complications - metabolism
dc.subject.meshFollow-Up Studies
dc.subject.meshGingival Crevicular Fluid - chemistry
dc.subject.meshGingivitis - metabolism
dc.subject.meshHumans
dc.subject.meshInflammation Mediators - analysis
dc.subject.meshInterleukin-1beta - analysis
dc.subject.meshInterleukin-8 - analysis
dc.subject.meshMatrix Metalloproteinase 8 - analysis
dc.subject.meshMatrix Metalloproteinase 9 - analysis
dc.subject.meshNucleic Acid Hybridization
dc.subject.meshPeriodontal Index
dc.subject.meshProspective Studies
dc.subject.meshYoung Adult
dc.titlePro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: A proof-of-concept study
dc.typeArticle
Author Affiliations
  1. Prince Philip Dental Hospital
  2. Universität Bern
  3. University of Michigan School of Dentistry