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Article: Pro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: A proof-of-concept study

TitlePro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: A proof-of-concept study
Authors
Issue Date2010
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
Citation
Journal Of Clinical Periodontology, 2010, v. 37 n. 1, p. 9-16 How to Cite?
AbstractAbstract Aim: To compare gingival crevicular fluid (GCF) biomarker levels and microbial distribution in plaque biofilm (SP) samples for subjects with type 1 diabetes (T1DM) versus healthy subjects without diabetes during experimental gingivitis (EG). Materials and Methods: A total of nine T1DM patients and nine healthy controls of age and gender similar to the T1DM patients were monitored for 35 days during EG. Hygiene practices were stopped for 3 weeks, and GCF, SP, plaque index (PI) and gingival index were determined. IL-1β, IL-8, MMP-8 and MMP-9 were quantified by enzyme-linked immunosorbent assay, and SP samples were assessed by DNA-DNA hybridization for a panel of 40 subgingival microbial species. Results: IL-1β levels in T1DM patients were elevated compared with healthy individuals, and showed differences between groups at 7-21 days while healthy patients showed IL-1β increases from baseline to 14-21 days (p<0.05). Differences were observed in MMP-9 levels between patients with and without T1DM at 7-14 days (p<0.05). Orange complex species and PI measurements displayed a superior correlation with biomarker levels when compared with other complexes or clinical measurements during EG. Conclusions: The mean GCF biomarker levels for IL-1β and MMP-8 were most significantly elevated in T1DM subjects compared with healthy individuals during EG, not resulting from differences in the mean PI or microbial composition. © 2009 John Wiley & Sons A/S.
Persistent Identifierhttp://hdl.handle.net/10722/66053
ISSN
2014 Impact Factor: 4.010
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSalvi, GEen_HK
dc.contributor.authorFranco, LMen_HK
dc.contributor.authorBraun, TMen_HK
dc.contributor.authorLee, Aen_HK
dc.contributor.authorRutger Persson, Gen_HK
dc.contributor.authorLang, NPen_HK
dc.contributor.authorGiannobile, WVen_HK
dc.date.accessioned2010-09-06T05:43:12Z-
dc.date.available2010-09-06T05:43:12Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Clinical Periodontology, 2010, v. 37 n. 1, p. 9-16en_HK
dc.identifier.issn0303-6979en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66053-
dc.description.abstractAbstract Aim: To compare gingival crevicular fluid (GCF) biomarker levels and microbial distribution in plaque biofilm (SP) samples for subjects with type 1 diabetes (T1DM) versus healthy subjects without diabetes during experimental gingivitis (EG). Materials and Methods: A total of nine T1DM patients and nine healthy controls of age and gender similar to the T1DM patients were monitored for 35 days during EG. Hygiene practices were stopped for 3 weeks, and GCF, SP, plaque index (PI) and gingival index were determined. IL-1β, IL-8, MMP-8 and MMP-9 were quantified by enzyme-linked immunosorbent assay, and SP samples were assessed by DNA-DNA hybridization for a panel of 40 subgingival microbial species. Results: IL-1β levels in T1DM patients were elevated compared with healthy individuals, and showed differences between groups at 7-21 days while healthy patients showed IL-1β increases from baseline to 14-21 days (p<0.05). Differences were observed in MMP-9 levels between patients with and without T1DM at 7-14 days (p<0.05). Orange complex species and PI measurements displayed a superior correlation with biomarker levels when compared with other complexes or clinical measurements during EG. Conclusions: The mean GCF biomarker levels for IL-1β and MMP-8 were most significantly elevated in T1DM subjects compared with healthy individuals during EG, not resulting from differences in the mean PI or microbial composition. © 2009 John Wiley & Sons A/S.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPEen_HK
dc.relation.ispartofJournal of Clinical Periodontologyen_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshBacteria - classificationen_HK
dc.subject.meshBiofilmsen_HK
dc.subject.meshBiological Markers - analysisen_HK
dc.subject.meshCase-Control Studiesen_HK
dc.subject.meshCohort Studiesen_HK
dc.subject.meshDental Plaque - microbiologyen_HK
dc.subject.meshDental Plaque Indexen_HK
dc.subject.meshDiabetes Mellitus, Type 1 - complications - metabolismen_HK
dc.subject.meshFollow-Up Studiesen_HK
dc.subject.meshGingival Crevicular Fluid - chemistryen_HK
dc.subject.meshGingivitis - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInflammation Mediators - analysisen_HK
dc.subject.meshInterleukin-1beta - analysisen_HK
dc.subject.meshInterleukin-8 - analysisen_HK
dc.subject.meshMatrix Metalloproteinase 8 - analysisen_HK
dc.subject.meshMatrix Metalloproteinase 9 - analysisen_HK
dc.subject.meshNucleic Acid Hybridizationen_HK
dc.subject.meshPeriodontal Indexen_HK
dc.subject.meshProspective Studiesen_HK
dc.subject.meshYoung Adulten_HK
dc.titlePro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: A proof-of-concept studyen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0303-6979&volume=37&spage=9&epage=16&date=2010&atitle=Pro-inflammatory+biomarkers+during+experimental+gingivitis+in+patients+with+type+1+diabetes+mellitus:+a+proof-of-concept+studyen_HK
dc.identifier.emailLang, NP:nplang@hkucc.hku.hken_HK
dc.identifier.authorityLang, NP=rp00031en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1600-051X.2009.01500.xen_HK
dc.identifier.pmid19958441en_HK
dc.identifier.scopuseid_2-s2.0-72549107943en_HK
dc.identifier.hkuros169087en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-72549107943&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume37en_HK
dc.identifier.issue1en_HK
dc.identifier.spage9en_HK
dc.identifier.epage16en_HK
dc.identifier.isiWOS:000272771700002-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridSalvi, GE=35600695300en_HK
dc.identifier.scopusauthoridFranco, LM=35279591600en_HK
dc.identifier.scopusauthoridBraun, TM=35426133200en_HK
dc.identifier.scopusauthoridLee, A=7405631555en_HK
dc.identifier.scopusauthoridRutger Persson, G=6507743898en_HK
dc.identifier.scopusauthoridLang, NP=7201577367en_HK
dc.identifier.scopusauthoridGiannobile, WV=7004619325en_HK
dc.identifier.citeulike6466146-

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