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Article: Pro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: A proof-of-concept study
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TitlePro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: A proof-of-concept study
 
AuthorsSalvi, GE2
Franco, LM3
Braun, TM3
Lee, A3
Rutger Persson, G2
Lang, NP2 1
Giannobile, WV3
 
Issue Date2010
 
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
 
CitationJournal Of Clinical Periodontology, 2010, v. 37 n. 1, p. 9-16 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-051X.2009.01500.x
 
AbstractAbstract Aim: To compare gingival crevicular fluid (GCF) biomarker levels and microbial distribution in plaque biofilm (SP) samples for subjects with type 1 diabetes (T1DM) versus healthy subjects without diabetes during experimental gingivitis (EG). Materials and Methods: A total of nine T1DM patients and nine healthy controls of age and gender similar to the T1DM patients were monitored for 35 days during EG. Hygiene practices were stopped for 3 weeks, and GCF, SP, plaque index (PI) and gingival index were determined. IL-1β, IL-8, MMP-8 and MMP-9 were quantified by enzyme-linked immunosorbent assay, and SP samples were assessed by DNA-DNA hybridization for a panel of 40 subgingival microbial species. Results: IL-1β levels in T1DM patients were elevated compared with healthy individuals, and showed differences between groups at 7-21 days while healthy patients showed IL-1β increases from baseline to 14-21 days (p<0.05). Differences were observed in MMP-9 levels between patients with and without T1DM at 7-14 days (p<0.05). Orange complex species and PI measurements displayed a superior correlation with biomarker levels when compared with other complexes or clinical measurements during EG. Conclusions: The mean GCF biomarker levels for IL-1β and MMP-8 were most significantly elevated in T1DM subjects compared with healthy individuals during EG, not resulting from differences in the mean PI or microbial composition. © 2009 John Wiley & Sons A/S.
 
ISSN0303-6979
2013 Impact Factor: 3.610
 
DOIhttp://dx.doi.org/10.1111/j.1600-051X.2009.01500.x
 
ISI Accession Number IDWOS:000272771700002
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorSalvi, GE
 
dc.contributor.authorFranco, LM
 
dc.contributor.authorBraun, TM
 
dc.contributor.authorLee, A
 
dc.contributor.authorRutger Persson, G
 
dc.contributor.authorLang, NP
 
dc.contributor.authorGiannobile, WV
 
dc.date.accessioned2010-09-06T05:43:12Z
 
dc.date.available2010-09-06T05:43:12Z
 
dc.date.issued2010
 
dc.description.abstractAbstract Aim: To compare gingival crevicular fluid (GCF) biomarker levels and microbial distribution in plaque biofilm (SP) samples for subjects with type 1 diabetes (T1DM) versus healthy subjects without diabetes during experimental gingivitis (EG). Materials and Methods: A total of nine T1DM patients and nine healthy controls of age and gender similar to the T1DM patients were monitored for 35 days during EG. Hygiene practices were stopped for 3 weeks, and GCF, SP, plaque index (PI) and gingival index were determined. IL-1β, IL-8, MMP-8 and MMP-9 were quantified by enzyme-linked immunosorbent assay, and SP samples were assessed by DNA-DNA hybridization for a panel of 40 subgingival microbial species. Results: IL-1β levels in T1DM patients were elevated compared with healthy individuals, and showed differences between groups at 7-21 days while healthy patients showed IL-1β increases from baseline to 14-21 days (p<0.05). Differences were observed in MMP-9 levels between patients with and without T1DM at 7-14 days (p<0.05). Orange complex species and PI measurements displayed a superior correlation with biomarker levels when compared with other complexes or clinical measurements during EG. Conclusions: The mean GCF biomarker levels for IL-1β and MMP-8 were most significantly elevated in T1DM subjects compared with healthy individuals during EG, not resulting from differences in the mean PI or microbial composition. © 2009 John Wiley & Sons A/S.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Clinical Periodontology, 2010, v. 37 n. 1, p. 9-16 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1600-051X.2009.01500.x
 
dc.identifier.citeulike6466146
 
dc.identifier.doihttp://dx.doi.org/10.1111/j.1600-051X.2009.01500.x
 
dc.identifier.epage16
 
dc.identifier.hkuros169087
 
dc.identifier.isiWOS:000272771700002
 
dc.identifier.issn0303-6979
2013 Impact Factor: 3.610
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid19958441
 
dc.identifier.scopuseid_2-s2.0-72549107943
 
dc.identifier.spage9
 
dc.identifier.urihttp://hdl.handle.net/10722/66053
 
dc.identifier.volume37
 
dc.languageeng
 
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/CPE
 
dc.publisher.placeDenmark
 
dc.relation.ispartofJournal of Clinical Periodontology
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdolescent
 
dc.subject.meshAdult
 
dc.subject.meshBacteria - classification
 
dc.subject.meshBiofilms
 
dc.subject.meshBiological Markers - analysis
 
dc.subject.meshCase-Control Studies
 
dc.subject.meshCohort Studies
 
dc.subject.meshDental Plaque - microbiology
 
dc.subject.meshDental Plaque Index
 
dc.subject.meshDiabetes Mellitus, Type 1 - complications - metabolism
 
dc.subject.meshFollow-Up Studies
 
dc.subject.meshGingival Crevicular Fluid - chemistry
 
dc.subject.meshGingivitis - metabolism
 
dc.subject.meshHumans
 
dc.subject.meshInflammation Mediators - analysis
 
dc.subject.meshInterleukin-1beta - analysis
 
dc.subject.meshInterleukin-8 - analysis
 
dc.subject.meshMatrix Metalloproteinase 8 - analysis
 
dc.subject.meshMatrix Metalloproteinase 9 - analysis
 
dc.subject.meshNucleic Acid Hybridization
 
dc.subject.meshPeriodontal Index
 
dc.subject.meshProspective Studies
 
dc.subject.meshYoung Adult
 
dc.titlePro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: A proof-of-concept study
 
dc.typeArticle
 
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Author Affiliations
  1. Prince Philip Dental Hospital
  2. Universität Bern
  3. University of Michigan School of Dentistry