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Article: Antibacterial Effect of Experimental Chlorhexidine-releasing Polymethyl Methacrylate-based Root Canal Sealers

TitleAntibacterial Effect of Experimental Chlorhexidine-releasing Polymethyl Methacrylate-based Root Canal Sealers
Authors
KeywordsAntimicrobial
chlorhexidine
direct contact test
Enterococcus faecalis
high-performance liquid chromatography
SuperBond sealer
Issue Date2009
PublisherElsevier Inc. The Journal's web site is located at http://www.jendodon.com
Citation
Journal Of Endodontics, 2009, v. 35 n. 9, p. 1255-1258 How to Cite?
AbstractIntroduction: This study evaluated the extent of chlorhexidine release from experimental chlorhexidine-containing polymethyl methacrylate (PMMA)-based root canal sealers prepared from a methacrylate-based sealer (SuperBond Sealer, Sun Medical Co Ltd, Shiga, Japan) and to examine its antimicrobial effect against Enterococcus faecalis using the direct contact test. Methods: Chlorhexidine diacetate powder was mixed with PMMA powder of SuperBond Sealer to obtain chlorhexidine concentrations of 1 wt%, 2 wt%, and 3 wt%, respectively. PMMA disks were fabricated by self-curing the experimental PMMA powder and pure SuperBond sealer (control) and immersing them in distilled water at 37 °C for 12 weeks. Chlorhexidine release was analyzed by high-performance liquid chromatography. The direct contact test was performed on wells of 96-well plates coated with the respective experimental root canal sealers (n = 6). Bacterial suspensions were placed directly on the sealers that were aged for 12 weeks. Results: The cumulative chlorhexidine release after 12 weeks of water immersion was largely dependent on the amount of chlorhexidine powder that was initially incorporated into the PMMA-based sealer. Kruskal-Wallis test revealed that chlorhexidine concentration had a significant effect on the cumulative release (p = 0.0005). Experimental sealers with the incorporation of 2 wt% and 3 wt% chlorhexidine exhibited sustained antimicrobial effect for at least 4 weeks and 8 weeks. Conclusion: The incorporation of 2wt% and 3wt% chlorhexidine in the PMMA-based sealer had a long lasing antimicrobial effect against Enterococcus faecalis as a result of the turbidimetric determination of bacterial growth. © 2009 American Association of Endodontists.
Persistent Identifierhttp://hdl.handle.net/10722/66048
ISSN
2015 Impact Factor: 2.904
2015 SCImago Journal Rankings: 1.681
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHiraishi, Nen_HK
dc.contributor.authorYiu, CKYen_HK
dc.contributor.authorKing, NMen_HK
dc.contributor.authorTay, FRen_HK
dc.date.accessioned2010-09-06T05:43:09Z-
dc.date.available2010-09-06T05:43:09Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Endodontics, 2009, v. 35 n. 9, p. 1255-1258en_HK
dc.identifier.issn0099-2399en_HK
dc.identifier.urihttp://hdl.handle.net/10722/66048-
dc.description.abstractIntroduction: This study evaluated the extent of chlorhexidine release from experimental chlorhexidine-containing polymethyl methacrylate (PMMA)-based root canal sealers prepared from a methacrylate-based sealer (SuperBond Sealer, Sun Medical Co Ltd, Shiga, Japan) and to examine its antimicrobial effect against Enterococcus faecalis using the direct contact test. Methods: Chlorhexidine diacetate powder was mixed with PMMA powder of SuperBond Sealer to obtain chlorhexidine concentrations of 1 wt%, 2 wt%, and 3 wt%, respectively. PMMA disks were fabricated by self-curing the experimental PMMA powder and pure SuperBond sealer (control) and immersing them in distilled water at 37 °C for 12 weeks. Chlorhexidine release was analyzed by high-performance liquid chromatography. The direct contact test was performed on wells of 96-well plates coated with the respective experimental root canal sealers (n = 6). Bacterial suspensions were placed directly on the sealers that were aged for 12 weeks. Results: The cumulative chlorhexidine release after 12 weeks of water immersion was largely dependent on the amount of chlorhexidine powder that was initially incorporated into the PMMA-based sealer. Kruskal-Wallis test revealed that chlorhexidine concentration had a significant effect on the cumulative release (p = 0.0005). Experimental sealers with the incorporation of 2 wt% and 3 wt% chlorhexidine exhibited sustained antimicrobial effect for at least 4 weeks and 8 weeks. Conclusion: The incorporation of 2wt% and 3wt% chlorhexidine in the PMMA-based sealer had a long lasing antimicrobial effect against Enterococcus faecalis as a result of the turbidimetric determination of bacterial growth. © 2009 American Association of Endodontists.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.jendodon.comen_HK
dc.relation.ispartofJournal of Endodonticsen_HK
dc.rightsThe Journal of Endodontics. Copyright © Lippincott Williams & Wilkins.en_HK
dc.subjectAntimicrobialen_HK
dc.subjectchlorhexidineen_HK
dc.subjectdirect contact testen_HK
dc.subjectEnterococcus faecalisen_HK
dc.subjecthigh-performance liquid chromatographyen_HK
dc.subjectSuperBond sealeren_HK
dc.subject.meshAnti-Infective Agents, Local - analysis - pharmacologyen_HK
dc.subject.meshChlorhexidine - analysis - pharmacologyen_HK
dc.subject.meshEnterococcus faecalis - drug effectsen_HK
dc.subject.meshLinear Modelsen_HK
dc.subject.meshMicrobial Viability - drug effectsen_HK
dc.subject.meshPolymethyl Methacrylate - chemistryen_HK
dc.subject.meshResin Cements - chemistryen_HK
dc.subject.meshRoot Canal Filling Materials - chemistryen_HK
dc.titleAntibacterial Effect of Experimental Chlorhexidine-releasing Polymethyl Methacrylate-based Root Canal Sealersen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0099-2399&volume=35&spage=1255&epage=8&date=2009&atitle=Antibacterial+effect+of+experimental+chlorhexidine-releasing+polymethyl+methacrylate-based+root+canal+sealers.+en_HK
dc.identifier.emailYiu, CKY: ckyyiu@hkucc.hku.hken_HK
dc.identifier.emailKing, NM: hhdbknm@hkucc.hku.hken_HK
dc.identifier.authorityYiu, CKY=rp00018en_HK
dc.identifier.authorityKing, NM=rp00006en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.joen.2009.06.019en_HK
dc.identifier.pmid19720226-
dc.identifier.scopuseid_2-s2.0-69249122554en_HK
dc.identifier.hkuros168460en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-69249122554&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume35en_HK
dc.identifier.issue9en_HK
dc.identifier.spage1255en_HK
dc.identifier.epage1258en_HK
dc.identifier.isiWOS:000269760600016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHiraishi, N=8925934400en_HK
dc.identifier.scopusauthoridYiu, CKY=7007115156en_HK
dc.identifier.scopusauthoridKing, NM=7201762850en_HK
dc.identifier.scopusauthoridTay, FR=7102091962en_HK

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